Ultralow temperature preservation method for capsicum pollens
A technology of ultra-low temperature preservation and pollen, applied in the direction of plant preservation, botanical equipment and methods, applications, etc., can solve the problem of flowering failure and achieve the effect of protection loss
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Embodiment 1
[0009] Relationship between different pretreatment, thawing methods and pollen vigor
[0010] When the conditions are convenient, it is better to pretreat the pollen at -20°C before storage, or directly put the freezing tube containing pepper pollen into liquid nitrogen without pretreatment; Significant difference, so choose to thaw directly indoors.
[0011] Table 1: The relationship between different pretreatments, different thawing methods and pollen viability of pollen stored in liquid nitrogen for one month (experimental time: 2009.2.18)
[0012]
Embodiment 2
[0014] Table 2: The relationship between different pretreatments, different thawing methods and pollen viability of pollen stored in liquid nitrogen for two months (experimental time: 2009.3.23)
[0015]
[0016] Note: In Table 1 and Table 2, the TTC staining method was used to measure the pollen viability, and three repeated slices were made for each treatment, and 5 fields of view were observed for each slice, and the average result was calculated
Embodiment 3
[0018] Table 3 The relationship between pollen stored in liquid nitrogen for half a year in different pretreatments, different thawing methods and pollen viability (experimental time: 2009.6.)
[0019]
[0020] Explanation: A: Represents pre-cooling at 4°C; B: Represents pre-cooling at -20°C; C: Represents direct input into liquid nitrogen at -196°C; due to high temperature in 2009, the pollination and seed setting rate was low; the above table 1 , 2, 3 The stored pollen was collected in Sanya, Hainan on January 15, 2009, and arrived in Changsha on January 17. It was put into a 2.0ml test tube on January 18, and put into liquid nitrogen after different treatments; 2009.6.16- On June 19, pollen was thawed at -20°C for 30 minutes, and on June 20-6.30, the pollen was thawed directly from liquid nitrogen and thawed naturally. Therefore, it is better to pre-cool at -20°C or put in directly when putting in. For instant noodles, choose to put in directly, and use natural thawing m...
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