Applications of a predominately gynoecious selfing line LC-03 in pumpkin breeding
A technology of LC-03, pumpkin, applied in the fields of application, botanical equipment and methods, plant genetic improvement, etc., can solve the problems of late breeding, incomplete emasculation, weakened disease resistance, etc., and achieve easy observation and quality assurance. , the effect of high purity
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Embodiment 1
[0029] Embodiment 1, the acquisition of strong female inbred line LC-03
[0030] In the early 1990s, Yu Zhenhua screened out a multi-female strain from farmer strains in Baoqing County, Heilongjiang, and named it Duozi 265 after several generations of self-purification. The characteristics of multi-seed 265: the rate of female flowers is particularly high, and the proportion of 2-3 female flowers in a leaf axil is relatively high, and the higher the proportion of female flowers is in the upper node of the plant, but there are still male flowers; the melon is gray-green skin, Oblate, white seeds, about 250 seeds per melon.
[0031] In 2003, a high-generation inbred line of Multiseed 265 had a variation. The mutant had male and hermaphrodite flowers before 10 nodes of the root, and then each leaf axil had female flowers, and male flowers no longer appeared. In view of this characteristic, Carry out self-pollination of a single plant, and harvest one mature melon at the end of S...
Embodiment 2
[0039] Embodiment 2, the application of strong female inbred line LC-03 (using the inbred line Duozi 265 as the male parent)
[0040] 1. Pumpkin breeding with strong female inbred line LC-03
[0041] 1. In 2007, the pollen of the inbred line Duozi 265 was granted to the strong female inbred line LC-03, managed normally, and seeds (F1 generation) were obtained.
[0042] 2. In 2008, the seeds obtained in step 1 were planted, the pollen of the male flowers was given to the female flowers, the growth was observed continuously under normal management, and the seeds (F2 generation) were harvested.
[0043] 2. Character analysis
[0044] 1. In May 2008, in Baoqing County, Heilongjiang, sow experimental seeds, manage them normally and observe the growth continuously. The experimental seeds were the F1 generation (seed), the strong female inbred line LC-03 (seed), and the inbred line Duozi 265 (seed), and they were treated in parallel.
[0045] 2. In October 2008, in Hainan Province...
Embodiment 3
[0070] Embodiment 3, the application of strong female inbred line LC-03 (using Xuecheng No. 1 or inbred line Qiang 8-1 as the male parent)
[0071] 1. Hybridization first and then selfing (using Syracuse No. 1 as the male parent)
[0072] The inbred line Duozi 265 was replaced by Xuecheng No. 1, and the others were the same as in Example 2.
[0073] The statistical results of the first female time of each parent in each year and the average node position of the first female flower are shown in Table 7 (the average value of 100 plants for each parent in each year). The statistical results of the first female time and the average node position of the first female flower in each year of the F1 generation are shown in Table 8 (the average value of 100 plants in each year). In the strong female inbred line LC-03, the first female flower appeared on average at 8.4 leaves, followed by one female flower per leaf, and no male flower appeared. On average, the first female flower appea...
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