Method for cultivating japonica rice varieties resistant to herbicide imazethapyr assisted by molecular marker

A molecular marker-assisted, herbicide-resistant technology, applied in biochemical equipment and methods, microbial measurement/inspection, etc., can solve problems such as economic loss, affecting crop growth and development, reducing crop yield, quality, etc., to achieve accuracy and simplify The effect of the process

Active Publication Date: 2017-10-27
JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

High-yield, low-cost, high-quality rice is currently the main breeding goal, among which weed damage is one of the main factors affecting yield. Weeds not only compete with crops for water and light, but also parasitize pests and diseases, affecting crop growth and development, reducing crop yield and quality. Affect harvesting efficiency and cause huge economic losses

Method used

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  • Method for cultivating japonica rice varieties resistant to herbicide imazethapyr assisted by molecular marker
  • Method for cultivating japonica rice varieties resistant to herbicide imazethapyr assisted by molecular marker
  • Method for cultivating japonica rice varieties resistant to herbicide imazethapyr assisted by molecular marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1. Acquisition of SSR Molecular Marker RM7413 Linked to Imazethapyr Herbicide Resistance in Japonica Rice

[0040] The research team of the present invention screened out an imazethapyr-resistant rice material "Jinjing 818" from 7403 rice variety resource materials. The variety Jinjing 818 was identified to be resistant to the herbicide imazethapyr, but its genetic mechanism was unknown. Therefore, the genetic mechanism of this variety was first studied and the resistance gene mapping was carried out.

[0041] 1) Genetic analysis of the herbicide imazethapyr resistance of Jinjing 818

[0042] The japonica rice variety Jinjing 818 resistant to the herbicide imazethapyr was used as a donor and the popularized japonica rice variety Suken 118 sensitive to the herbicide imazethapyr was used as a recipient to obtain F 1 10 plants, to F 1 When the generation seedling grows to 2 leaves and 1 heart, the recommended concentration of imazethapyr is 70g ai ha -1 Spray scre...

Embodiment 2

[0052] Example 2. Application of Molecular Markers in Assisted Breeding of Imazethapyr-resistant Japonica Rice Varieties

[0053] 1) The present invention uses the imazethapyr-resistant variety Jinjing 818 as the donor parent, and the imazethapyr-sensitive Nanjing 9108 as the recipient parent. Both parents are currently the main popularized varieties with good yield and adaptability. The two parents were crossed and combined to harvest F 1 generation seeds.

[0054] 2)F 1 F 2 Generation seeds, planted F 2 50 seeds, get F 2 Substitute plants. Leaves were taken from each individual plant, DNA was extracted, and the SSR marker RM7413 in Example 1 was used for amplification. The amplification system, conditions and detection methods were the same as those shown in Example 1. Select a single plant that is completely consistent with the band pattern of the resistant parent.

[0055] 3) Change F 2 Generationally selected plants were continuously selfed to F 7 At the same tim...

Embodiment 3

[0056] Example 3. The application potential of the marker RM7413 linked to the herbicide-resistant imazethapyr in japonica rice in the currently popularized japonica rice varieties

[0057] Conventional japonica rice varieties usually do not have the ability to resist the herbicide imazethapyr. If the marker RM7413 has the polymorphisms in Examples 1 and 2 in the herbicide-sensitive conventional japonica rice variety and the resistant variety Jinjing 818, then RM7413 can be used in conventional japonica rice varieties. Improvement of herbicide resistance in japonica rice to obtain new herbicide-resistant varieties. The present invention selects 91 japonica rice varieties mainly popularized in the middle and lower reaches of the Yangtze River for polymorphic identification, and uses the SSR marker RM7413 in Examples 1 and 2 to amplify, and the amplification system, conditions and detection methods are the same as those shown in Example 1 . The identified varieties include Lian...

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Abstract

The invention relates to the technical field of biology, and in particular to a method for cultivating a japonica rice variety resistant to herbicide imazethapyr assisted by molecular marker. The method comprises the steps of screening an anti-imazethapyr rice material Jinjing 818 from 7403 parts of rice varieties resource materials, taking Jinjing 818 of japonica rice variety resistant to herbicide imazethapyr as a donor and conventional japonica rice variety as an acceptor for crossbreeding, obtaining F2 generation group through F1 hybrid selfing, using SSR molecular marker RM7413 to choose an individual corresponding to a linked molecular marker band of the donor Jinjing 818 herbicide resistance gene, namely homozygous genotype F2 generation strain and obtaining the stable japonica rice new variety resistant to the herbicide imazethapyr through F2 generation strain continuous selfing until obtain high-generation groups. The SSR molecular marker RM7413 and the molecular marker assisted cultivation method obtained by the invention will play an important role in genetic improvement of the japonica rice resistant to the herbicide imazethapyr.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for assisting the cultivation of herbicide-resistant imazethapyr japonica rice varieties by using SSR molecular markers. Background technique [0002] Rice is one of the main foods for about 50% of the world's population, and it is the main source of people's daily carbohydrates, proteins, vitamins, etc. The increase of population, the reduction of arable land, the change of planting structure, the shortage of labor force and water source will cause the global food crisis. High-yield, low-cost, high-quality rice is currently the main breeding goal, among which weed damage is one of the main factors affecting yield. Weeds not only compete with crops for water and light, but also parasitize pests and diseases, affecting crop growth and development, reducing crop yield and quality. Harvesting efficiency is affected, resulting in huge economic losses. Using herbicides and plan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 杨杰费云燕王芳权范方军王军仲维功李文奇许杨
Owner JIANGSU ACADEMY OF AGRICULTURAL SCIENCES
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