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A method for breeding japonica rice varieties resistant to herbicide imazethapyr using molecular markers

A molecular marker-assisted, herbicide-resistant technology, applied in the direction of biochemical equipment and methods, microbial measurement/inspection, etc., can solve problems affecting crop growth and development, affecting harvesting efficiency, economic losses, etc.

Active Publication Date: 2020-07-07
JIANGSU ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

High-yield, low-cost, high-quality rice is currently the main breeding goal, among which weed damage is one of the main factors affecting yield. Weeds not only compete with crops for water and light, but also parasitize pests and diseases, affecting crop growth and development, reducing crop yield and quality. Affect harvesting efficiency and cause huge economic losses

Method used

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  • A method for breeding japonica rice varieties resistant to herbicide imazethapyr using molecular markers
  • A method for breeding japonica rice varieties resistant to herbicide imazethapyr using molecular markers
  • A method for breeding japonica rice varieties resistant to herbicide imazethapyr using molecular markers

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0039] Example 1. Acquisition of the SSR molecular marker RM7413 linked to the herbicide resistance of japonica rice

[0040] The research group of the present invention screened out a rice material "Jinjing 818" which is resistant to imazethapyr from 7403 rice variety resource materials. After identification, it was found that the variety Jinjing 818 was resistant to the herbicide Imazethapyr, but its genetic mechanism was unknown. Therefore, the genetic mechanism of this variety was first studied, and the resistance gene mapping was carried out.

[0041] 1) Genetic analysis of Jinjing 818 resistance to the herbicide Imazethapyr

[0042] The japonica rice variety Jinjing 818 resistant to the herbicide imazethapyr was used as the donor, and the popularized japonica rice variety Suken 118 sensitive to the herbicide imazethapyr was used as the acceptor to obtain F. 1 10 plants, to F 1 When the seedlings grow to 2 leaves and 1 heart, the recommended concentration of imazethapyr i...

Embodiment 2

[0052] Example 2. Application of molecular markers in assisted breeding of imazamox-resistant japonica rice varieties

[0053] 1) The present invention uses imazethapyr-resistant variety Jinjing 818 as the donor parent, and imazethapyr-sensitive Nanjing 9108 as the recipient parent. Both parents are currently the main popular varieties with good yield and adaptability. The two parents are crossed and assembled to harvest F 1 Substitute seeds.

[0054] 2)F 1 F was obtained by selfing the progeny plant 2 Substitute seeds, plant F 2 50 seeds to get F 2 Substitute plants. Take leaves from each individual plant, extract DNA, and use the SSR marker RM7413 in Example 1 for amplification. The amplification system, conditions and detection methods are the same as those shown in Example 1. Individual plants that are completely consistent with the resistant parent's banding pattern are selected.

[0055] 3) Put F 2 Generation-selected plants were continuously selfed to F 7 At th...

Embodiment 3

[0056] Example 3. Application potential of the marker RM7413 linked to the japonica resistance herbicide imazethapyr in the currently popular japonica rice varieties

[0057] Conventional japonica rice varieties usually do not have the ability to resist the herbicide imazethapyr. If the marker RM7413 has the polymorphisms in Examples 1 and 2 in the herbicide-sensitive conventional japonica rice varieties and the resistant variety Jinjing 818, then RM7413 can be used in conventional japonica rice varieties. Herbicide resistance improvement of japonica rice to obtain new herbicide-resistant varieties. In the present invention, 91 japonica rice varieties mainly promoted in the middle and lower reaches of the Yangtze River are selected for polymorphism identification, and the SSR marker RM7413 in Examples 1 and 2 is used for amplification. The amplification system, conditions and detection methods are the same as those shown in Example 1. . The identified varieties include Lianji...

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Abstract

The invention relates to the technical field of biology, and in particular to a method for cultivating a japonica rice variety resistant to herbicide imazethapyr assisted by molecular marker. The method comprises the steps of screening an anti-imazethapyr rice material Jinjing 818 from 7403 parts of rice varieties resource materials, taking Jinjing 818 of japonica rice variety resistant to herbicide imazethapyr as a donor and conventional japonica rice variety as an acceptor for crossbreeding, obtaining F2 generation group through F1 hybrid selfing, using SSR molecular marker RM7413 to choose an individual corresponding to a linked molecular marker band of the donor Jinjing 818 herbicide resistance gene, namely homozygous genotype F2 generation strain and obtaining the stable japonica rice new variety resistant to the herbicide imazethapyr through F2 generation strain continuous selfing until obtain high-generation groups. The SSR molecular marker RM7413 and the molecular marker assisted cultivation method obtained by the invention will play an important role in genetic improvement of the japonica rice resistant to the herbicide imazethapyr.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for assisted breeding of herbicide-resistant imazethapyr japonica rice varieties using SSR molecular markers. Background technique [0002] Rice is one of the main food for about 50% of the world's population, and is the main source of people's daily carbohydrates, proteins, vitamins, etc. The increase of population, the reduction of cultivated land, the change of planting structure, and the lack of labor and water resources will cause a global food crisis. High-yield, low-cost, and high-quality rice is the main breeding goal at present, among which weeds are one of the main factors affecting yield. Weeds not only compete with crops for water and light, but also parasitic diseases and insect pests, affecting crop growth and development, reducing crop yield and quality. Affect harvesting efficiency and cause huge economic losses. Using herbicides and planting herbicide-resi...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6895
CPCC12Q1/6895C12Q2600/13C12Q2600/156
Inventor 杨杰费云燕王芳权范方军王军仲维功李文奇许杨
Owner JIANGSU ACAD OF AGRI SCI
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