Method of using anther culture rapidly selecting wheat photo-thermoperiod sensitive genic male sterile lines

Abstract

The invention belongs to the fields of crop science and crop breeding, which precisely provides a method for cultivating a rapid selection new PTGMS line in wheat with anthers. The method comprises the following steps: 1) hybridizing a PTGMS line needing improving with a conventional wheat breed, with a PTGMS line needing improving as a maternal plant and a conventional wheat breed as a father plant, to acquire an F1 hybrid; 2) seeding the acquired F1 hybrids or the selected F3 seeds; 3) carrying out anther inoculation when the microspores of plant F1 or F3 develop to monocytes in a middle-advanced stage, to acquire a haploid; 4) carrying out chromosome doubling to the haploid acquired in step 3), to acquire a homozygous diploid; and; 5) acquiring the seed of the homozygous diploid and dividing the seed of the homozygous diploid into two equal shares, with one share being planted in an ecological environment to appraise the agronomic characters of the species and breed the seeds, with the other share being planted in a sterile environment to appraise the fertility of the species, thus selectively breeding the PTGMS line in wheat with the needed agronomic characters.

Description

technical field [0001] The invention belongs to the fields of crop science and crop breeding. Specifically, the invention relates to a method for rapidly selecting and breeding new wheat light-temperature-sensitive sterile lines by using anther culture. Background technique [0002] Wheat is the largest food crop with the widest distribution in the world. The perennial planting area in my country is about 400 million mu, second only to rice. Wheat is a self-pollinated crop, and the use of heterosis is considered to be an important way to increase wheat yield in the 21st century. Over the years, countries have been trying to find ways and methods to develop and utilize their heterosis. At present, there are three main ways to utilize heterosis in wheat: the utilization of nuclear-cytoplasmic interaction male sterility (three-line method), the use of chemical male sterility (chemical killing method) and the use of photothermo-sensitive male sterility (two-line method). Due t...

AI Technical Summary

Problems solved by technology

The present invention aims to overcome the disadvantages of conventional techniques such as long breeding cycle, difficult operation, inability to balance fertility identification and selection of sterile offspring, and provides a new method for rapidly transforming wheat light-temperature-sensitive male sterile line resources, enabling the transformation from hybridization to It only takes 4 years to transform into a new CMS resource

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