Breeding method for rapidly obtaining cytoplasm male sterility line and maintenance line of carmine radish
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A technology of cytoplasmic male sterility and rouge radish, applied in the fields of botanical equipment and methods, application, plant gene improvement, etc., can solve the problems such as the lack of detailed elaboration of the breeding process of male sterile lines and maintainer lines, and achieve heterosis Significant, well-developed, good synergistic effect
Inactive Publication Date: 2014-11-26
单县秋歌农产品有限公司
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Lu Fafa et al. (Chinese Vegetables, 2012(2): 104-106) and Tao Hongying et al. (Chinese Vegetables, 2012(1): 37) respectively prepared Rouge 1 and Rouge 2 Red Heart by using the male sterile lines selected. Radish hybrids, but the selection process for male sterile lines and maintainer lines is not elaborated
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Embodiment 1
[0027] The self-retained species "Subei Carrot" from Jiangsu was selected, with a flat leaf type, rich in red pigment in the fleshy root bark and fleshy part; the radish cytoplasmic male sterile line D-1 was selected as the sterile source, with a flat leaf type, Red skin and white heart.
[0028] 1. After the rouge radish variety "Subei Carrot" is sown, observe the separation and change of offspring traits after the first generation of selfing. A good breeding goal is to select a single plant and save the seed for the second generation of selfing.
[0029] 2. In the second-generation population of self-crossing, according to the breeding goal, continue to select individual plants for self-purification, and select individual plants for the third-generation self-crossing.
[0030] 3. At the same time, according to the breeding goal, look for the radish cytoplasmic male sterile line material D-1 of the same species that is closer to the target traits as the female parent, and us...
Embodiment 2
[0039] The commercial variety "Ranzhihong 1" from Sichuan was selected, with split leaf type, rich in red pigment in the fleshy root bark and fleshy part; the radish cytoplasmic male sterile line material C-1 was selected as the sterile source, with split leaf type, White skin and white heart.
[0040] 1. After sowing the rouge radish variety "Ranzhihong 1", observe the separation and change of offspring traits after the first generation of self-crossing. The goal of breeding with good compatibility is to select a single plant and save the seed for the second generation of selfing.
[0041] 2. In the second-generation population of self-crossing, according to the breeding goal, continue to select individual plants for self-purification, and select individual plants for the third-generation self-crossing.
[0042]3. At the same time, according to the breeding goal, look for the radish cytoplasmic male sterile line material D-1 of the same species that is closer to the target t...
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Abstract
The invention discloses a breeding method for rapidly obtaining a cytoplasm male sterility line and a maintenance line of carmine radish. The method mainly comprises the following steps: selecting different types of carmine radish materials, performing first generation selfing, determining a selective breeding object, performing orientation selection on a single plant, and performing second generation selfing; performing orientation selection on the single plant for continuously selfing in a selfing offspring group, and performing orientation selection on the single plant according to the selective breeding object; selecting cytoplasm male sterility as a female parent, selecting the selected single plant as a male parent, performing interspecies cross, obtaining hybrid F1, thereby transferring CMS genes of the sterility to collard; continuously selfing and purifying the selfing offspring, and screening a sterile plant which is the closest to the selective breeding object of the male parent to serve as a female parent in a cytoplasm male sterility hybrid plant group, taking the screen single plant which is the closest to the selective breeding object in the selfing offspring as a male parent for continuously performing paired cross; and purifying the selfing offspring, transferring the male sterility, simultaneously performing paired cross on each generation, and selfing multiple generations to obtain the maintenance line and the male sterility line. According to the method, the labor and cost can be obviously reduced, and the breeding time is shortened.
Description
technical field [0001] The invention relates to a breeding method of rouge radish, in particular to a breeding method for quickly obtaining a cytoplasmic male sterile line and a maintainer line of rouge radish, and belongs to the technical field of plant breeding. Background technique [0002] Rouge radish (Raphanus Sativus L.Sinoruber Makino), also known as red radish, belongs to a local variety of radish in the family Brassicaceae. It is famous for its red carpel and rich in anthocyanins. It is mainly produced in Fuling District, Chongqing City, near It is also cultivated in cities and counties, Sichuan, Guizhou and other provinces. In recent years, many units have extracted natural anthocyanins from the fleshy root of rouge radish. Radish red pigment is a kind of natural food coloring, which is safe, non-toxic, rich in resources, and has certain nutritional and pharmacological effects. It is one of the natural pigments allowed to be used by the National Additive Standard...
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