The invention provides a chondroitin sulfate production process. A raw material is crushed to form particles with a diameter of 3-5mm, the particles are placed into an immersion pool, water which is 7-8 times as heavy as the raw material is added into the immersion pool for immersion, the particles are sent into a pulping machine to form a raw material pulp, the raw material pulp is sent into a reaction kettle, is heated and boils, then heat preservation is carried out for 10-15 minutes, and floating oil is taken out after temperature reduction to obtain collagen thermal denaturation liquid; a PH value of thermal denaturation liquid caustic soda is adjusted, bacterial enzymes are added, heat-preservation enzymolysis is carried out for 5-6 hours, the temperature is reduced to be the same as the temperature of cold water, then the temperature is raised to 70-75 DEG C, and heat-preservation is carried out for 10-15 minutes; materials after enzymolysis are clarified and filtered, and a wet-state chondroitin course product is obtained after alcohol precipitation, dehydration and alcohol removal; decoloration, concentration, alcohol precipitation and alcohol removal are carried out on the wet-state chondroitin course product, and a chondroitin fine product is obtained after drying. Mechanical physical degradation is carried out, the chondroitin is prevented from being degraded under a high temperature, the yield rate is improved, production cost is reduced, the yield is improved, the production efficiency is improved, the production is increased and the process is scientific and environmentally friendly.