Breeding method of cytoplasmic male sterile early-maturing Chinese cabbage germplasm material

A technology for male sterility and Chinese cabbage, which is applied in the field of selection and breeding of cytoplasmic male sterile early-maturing Chinese cabbage germplasm materials, can solve the problems of difficult seed production technology, high cost of parental seeds, and difficulty in obtaining hybrids, and achieves functions Normal, short period, strong resistance effect

Active Publication Date: 2017-07-18
河南省农业科学院园艺研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the end of the 1960s, domestic research began to use self-incompatibility to produce F1 hybrids and applied them to production. However, since the propagation of self-incompatibility lines mainly relies on artificial pollination at the bud stage, multi-generational self-trading has led to decline in viability and cost of parental seeds. High, difficult seed production technology and difficult to achieve 100% hybrids and other defects, and the use of male sterile lines to produce a generation of hybrids can solve the above problems

Method used

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  • Breeding method of cytoplasmic male sterile early-maturing Chinese cabbage germplasm material
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  • Breeding method of cytoplasmic male sterile early-maturing Chinese cabbage germplasm material

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Embodiment 1

[0036] One of the breeding methods of a cytoplasmic male sterile early-maturing Chinese cabbage germplasm material, comprising the following steps:

[0037] (1) The precocious self-compatibility line backcross male parent Y231-330 (genotype AA) was obtained by using the free microspore culture method (preserved in the mid-term seed resource bank of Henan Academy of Agricultural Sciences, preservation number: S01A0057);

[0038] (2) The female parent of the cytoplasmic male sterile material oguraCMS (its genotype is AACC) of Brassica napus, and the backcross male parent of the Chinese cabbage early maturing self-compatibility line Y231-330 obtained in step (1) as the male parent , through interspecific hybridization to obtain the first-generation seed F 1 (genotype AAC);

[0039] (3) the first generation seed F obtained in field sowing step (2) 1 , select the excellent sterile individual plant whose performance traits in the field are consistent with the backcross male parent...

Embodiment 2

[0052] A second method of breeding cytoplasmic male sterile early-maturing Chinese cabbage germplasm materials, comprising the following steps:

[0053] (1) Obtain the precocious self-compatibility line backcross male parent Y66-9 (preserved in the medium-term seed resource bank of Henan Academy of Agricultural Sciences, preservation number S01A0061) (its genotype AA) by adopting the free microspore culture method;

[0054] (2) Taking the cytoplasmic male sterile material oguraCMS (its genotype AACC) of Brassica napus as the female parent, and the Chinese cabbage precocious self-compatibility backcross male Y66-9 obtained in step (1) as the male parent, Obtaining the first-generation seed F through interspecific hybridization 1 (genotype AA);

[0055] (3) the first generation seed F obtained in field sowing step (2) 1 , select an excellent sterile individual plant with the same field performance traits as the backcross male parent Y66-9 (all sterility of the offspring) as th...

Embodiment 3

[0068] A method for breeding cytoplasmic male sterile early-maturing Chinese cabbage germplasm materials, comprising the following steps:

[0069] (1) The precocious self-compatibility line backcross male parent ZY15 (preserved in the mid-term seed resource bank of Henan Academy of Agricultural Sciences, preservation number S01A0060) (genotype AA) was obtained by adopting the free microspore culture method;

[0070] (2) Using the cytoplasmic male sterile material oguraCMS (genotype AACC) of Brassica napus as the female parent and the Chinese cabbage precocious self-compatibility line ZY15 obtained in step (1) as the male parent, the interspecific F 1 (genotype AA);

[0071] (3) the first generation seed F obtained in field sowing step (2) 1 , select an excellent sterile individual plant with the same field performance traits as the backcross male parent ZY15 as the next round of backcross female parent;

[0072] (4) Using the backcross female parent selected in step (3) and...

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Abstract

The invention discloses a breeding method of a cytoplasmic male sterile early-maturing Chinese cabbage germplasm material. The method comprises the steps of: with a brassica napus cytoplasmic male sterile material oguraCMS as a female parent and a Chinese cabbage precocious affinity early-maturing compatible line as a male parent, carrying out interspecific hybridization to obtain the first generation of bred seeds; and selecting superior individual plants with normal nectar but without aborted bud similar to the breeding male parent as the next round of backcross female parent, carrying out continuous seven generations of backcross breeding, carrying out field character selection to breed the cytoplasmic male sterile Chinese cabbage germplasm material TYMS231-330 which has stable sterility, and of which the sterile plant rate and the sterile degree reach 100% and the character is completely consistent with that of a corresponding maintainer line. According to the obtained sterile germplasm material TYMS231-330, the advantage of the male sterility of ogura cytoplasmic sterile source Chinese cabbage can be kept, the defects such as yellowing of plant leaves, nectary degradation and poor combining ability can be overcome, bees and the like can be attracted for normal pollination, the comprehensive resistance is high and the use value is high.

Description

technical field [0001] The invention belongs to the technical field of cruciferous vegetable breeding, and in particular relates to a method for breeding germplasm materials of cytoplasmic male sterile early-maturing Chinese cabbage. [0002] technical background [0003] Chinese cabbage is one of the vegetables with the largest planting area in my country and one of the vegetables with the largest winter storage in the north. It plays a pivotal role in balancing market supply and stabilizing vegetable prices. Chinese cabbage also has the characteristics of tender meat, light and delicious taste, rich nutrition, and wide adaptability, and is deeply loved by consumers. [0004] With the improvement of people's living standards and the ever-changing needs of consumers, people hope to eat Chinese cabbage, a "national dish" that is fresh, delicious, nutritious, and diverse in color and variety in every season. Breeding of new varieties should be closely integrated with the marke...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 姚秋菊张晓伟原玉香赵艳艳魏小春王志勇
Owner 河南省农业科学院园艺研究所
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