Breeding method of cytoplasmic male sterile early-maturing Chinese cabbage germplasm material
A technology for male sterility and Chinese cabbage, which is applied in the field of selection and breeding of cytoplasmic male sterile early-maturing Chinese cabbage germplasm materials, can solve the problems of difficult seed production technology, high cost of parental seeds, and difficulty in obtaining hybrids, and achieves functions Normal, short period, strong resistance effect
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Embodiment 1
[0036] One of the breeding methods of a cytoplasmic male sterile early-maturing Chinese cabbage germplasm material, comprising the following steps:
[0037] (1) The precocious self-compatibility line backcross male parent Y231-330 (genotype AA) was obtained by using the free microspore culture method (preserved in the mid-term seed resource bank of Henan Academy of Agricultural Sciences, preservation number: S01A0057);
[0038] (2) The female parent of the cytoplasmic male sterile material oguraCMS (its genotype is AACC) of Brassica napus, and the backcross male parent of the Chinese cabbage early maturing self-compatibility line Y231-330 obtained in step (1) as the male parent , through interspecific hybridization to obtain the first-generation seed F 1 (genotype AAC);
[0039] (3) the first generation seed F obtained in field sowing step (2) 1 , select the excellent sterile individual plant whose performance traits in the field are consistent with the backcross male parent...
Embodiment 2
[0052] A second method of breeding cytoplasmic male sterile early-maturing Chinese cabbage germplasm materials, comprising the following steps:
[0053] (1) Obtain the precocious self-compatibility line backcross male parent Y66-9 (preserved in the medium-term seed resource bank of Henan Academy of Agricultural Sciences, preservation number S01A0061) (its genotype AA) by adopting the free microspore culture method;
[0054] (2) Taking the cytoplasmic male sterile material oguraCMS (its genotype AACC) of Brassica napus as the female parent, and the Chinese cabbage precocious self-compatibility backcross male Y66-9 obtained in step (1) as the male parent, Obtaining the first-generation seed F through interspecific hybridization 1 (genotype AA);
[0055] (3) the first generation seed F obtained in field sowing step (2) 1 , select an excellent sterile individual plant with the same field performance traits as the backcross male parent Y66-9 (all sterility of the offspring) as th...
Embodiment 3
[0068] A method for breeding cytoplasmic male sterile early-maturing Chinese cabbage germplasm materials, comprising the following steps:
[0069] (1) The precocious self-compatibility line backcross male parent ZY15 (preserved in the mid-term seed resource bank of Henan Academy of Agricultural Sciences, preservation number S01A0060) (genotype AA) was obtained by adopting the free microspore culture method;
[0070] (2) Using the cytoplasmic male sterile material oguraCMS (genotype AACC) of Brassica napus as the female parent and the Chinese cabbage precocious self-compatibility line ZY15 obtained in step (1) as the male parent, the interspecific F 1 (genotype AA);
[0071] (3) the first generation seed F obtained in field sowing step (2) 1 , select an excellent sterile individual plant with the same field performance traits as the backcross male parent ZY15 as the next round of backcross female parent;
[0072] (4) Using the backcross female parent selected in step (3) and...
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