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A kind of breeding method of cytoplasmic male sterile early-maturing Chinese cabbage germplasm material

A technology of male sterility and Chinese cabbage, which is applied in the field of breeding germplasm materials of cytoplasmic male sterility and early-maturing Chinese cabbage, can solve the problems of difficult seed production technology, high cost of parental seeds, and difficulty in obtaining hybrids, and achieve functional Normal, short duration, strong resistance effect

Active Publication Date: 2019-06-28
河南省农业科学院园艺研究所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At the end of the 1960s, domestic research began to use self-incompatibility to produce F1 hybrids and applied them to production. However, since the propagation of self-incompatibility lines mainly relies on artificial pollination at the bud stage, multi-generational self-trading has led to decline in viability and cost of parental seeds. High, difficult seed production technology and difficult to achieve 100% hybrids and other defects, and the use of male sterile lines to produce a generation of hybrids can solve the above problems

Method used

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  • A kind of breeding method of cytoplasmic male sterile early-maturing Chinese cabbage germplasm material
  • A kind of breeding method of cytoplasmic male sterile early-maturing Chinese cabbage germplasm material
  • A kind of breeding method of cytoplasmic male sterile early-maturing Chinese cabbage germplasm material

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Experimental program
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Effect test

Embodiment 1

[0035] One of the breeding methods of a cytoplasmic male sterile early-maturing Chinese cabbage germplasm material, comprising the following steps:

[0036] (1) The backcross male parent Y231-330 (genotype AA) of the precocious self-incompatible line was obtained by using the free microspore culture method (preserved in the mid-term seed resource bank of Henan Academy of Agricultural Sciences, preservation number: S01A0057);

[0037] (2) Using the cytoplasmic male sterile material oguraCMS (its genotype is AACC) of Brassica napus as the female parent, and the Chinese cabbage precocious self-incompatible line Y231-330 obtained in step (1) as the male parent Ben, the first-generation seed F was obtained through interspecific hybridization 1 (genotype AAC);

[0038] (3) the first generation seed F obtained in field sowing step (2) 1 , select the excellent sterile individual plant whose performance traits in the field are consistent with the backcross male parent described in st...

Embodiment 2

[0051] A second method of breeding cytoplasmic male sterile early-maturing Chinese cabbage germplasm materials, comprising the following steps:

[0052] (1) The precocious self-incompatible line backcross male parent Y66-9 (preserved in the mid-term seed resource bank of Henan Academy of Agricultural Sciences, preservation number S01A0061) (its genotype AA) was obtained by adopting the free microspore culture method;

[0053] (2) The cytoplasmic male sterile material oguraCMS (its genotype AACC) of Brassica napus was used as the female parent, and the male parent of the early-maturing self-incompatible line Y66-9 of Chinese cabbage obtained in step (1) was used as the male parent , through interspecific hybridization to obtain the first-generation seed F 1 (genotype AA);

[0054] (3) the first generation seed F obtained in field sowing step (2) 1 , select an excellent sterile individual plant with the same field performance traits as the backcross male parent Y66-9 (all ster...

Embodiment 3

[0067] A method for breeding cytoplasmic male sterile early-maturing Chinese cabbage germplasm materials, comprising the following steps:

[0068] (1) Obtain the precocious self-incompatibility backcross male parent ZY15 (preserved in the medium-term seed resource bank of Henan Academy of Agricultural Sciences, preservation number S01A0060) (genotype AA) by adopting the free microspore culture method;

[0069] (2) Taking the cytoplasmic male sterile material oguraCMS (genotype AACC) of Brassica napus as the female parent, and the Chinese cabbage precocious self-incompatible line ZY15 obtained in step (1) as the male parent, through breeding F 1 (genotype AA);

[0070] (3) the first generation seed F obtained in field sowing step (2) 1 , select an excellent sterile individual plant with the same field performance traits as the backcross male parent ZY15 as the next round of backcross female parent;

[0071] (4) Using the backcross female parent selected in step (3) and the b...

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Abstract

The invention discloses a method for breeding germplasm materials of cytoplasmic male sterile early-maturing Chinese cabbage. The female parent is the cytoplasmic male sterile material oguraCMS of Brassica napus, and the early-maturing affinity line of Chinese cabbage is used as the male parent. Through interspecific hybridization The seeds of the first generation of transduction were obtained, and an excellent individual plant with normal nectary glands and no bud failure similar to the transgenic male parent was selected as the female parent of the next round of backcrossing. The Chinese cabbage cytoplasmic male sterile germplasm material TYMS231‑330 was bred with stable sterility, 100% sterile plant rate and sterility degree, and completely consistent characters with the corresponding maintainer line. The obtained sterile line germplasm material TYMS231-330 can not only maintain the advantages of male sterility of ogura cytoplasmic sterile source Chinese cabbage, but also overcome the defects of its plant leaves such as yellow flowers, nectary degeneration and poor combining ability, and can attract bees, etc. Normal pollination, strong comprehensive resistance, high utilization value.

Description

technical field [0001] The invention belongs to the technical field of cruciferous vegetable breeding, and in particular relates to a method for breeding germplasm materials of cytoplasmic male sterile early-maturing Chinese cabbage. technical background [0002] Chinese cabbage is one of the vegetables with the largest planting area in my country and one of the vegetables with the largest winter storage in the north. It plays a pivotal role in balancing market supply and stabilizing vegetable prices. Chinese cabbage also has the characteristics of tender meat, light and delicious taste, rich nutrition, and wide adaptability, and is deeply loved by consumers. [0003] With the improvement of people's living standards and the ever-changing needs of consumers, people hope to eat Chinese cabbage, a "national dish" that is fresh, delicious, nutritious, and diverse in color and variety in every season. Breeding of new varieties should be closely integrated with the market, and c...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 姚秋菊张晓伟原玉香赵艳艳魏小春王志勇
Owner 河南省农业科学院园艺研究所
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