Lily ball breeding method for fresh cutting flower production
A technique for fresh-cut flowers and lilies, applied in the field of lily bulb breeding, can solve problems such as constraints, inability to cultivate widely, poor propagation and cultivation techniques, etc.
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Embodiment 1
[0018] The method for breeding lily bulbs for producing fresh cut flowers, the specific steps are as follows:
[0019] 1) Put the first-generation commercial bulbs imported from the Netherlands at a constant temperature of 25°C. When the buds grow to 2cm, move them to 45°C for 40 days. At this time, the buds are about 6cm long;
[0020] 2) After removing the shoot apex for routine disinfection, use a binocular dissecting microscope to take a shoot apex meristem with a size of about 0.5 mm on the sterile operating table;
[0021] 3) Add 1.0 mg / l of 6-benzylaminoadenine (BA), 1.0 mg / l of naphthaleneacetic acid (NAA), 50 g / l of sucrose to the MS medium mother solution, and then add 200 μmol / l of 1-β-D -Ribofuranose-1,2,4-triazole (ribavirin) was uniformly mixed to obtain a lily induction medium, and the shoot tip meristem was inoculated in the lily induction medium for induction, and a large number of clustered buds could be differentiated after 45 days;
[0022] 4) Divide 5 bud...
Embodiment 2
[0028] The difference from Example 1 is:
[0029] The method for breeding lily bulbs for producing fresh cut flowers, the specific steps are as follows:
[0030] 1) Put the commercial bulbs at a constant temperature of 22°C. When the buds grow to 1cm, move them to 40°C for 20 days. At this time, the buds are about 5cm long;
[0031] 2) After removing the shoot tip for routine disinfection, use a binocular dissecting microscope to take a shoot tip meristem with a size of 0.1 mm on the sterile operating table;
[0032] 3) Add 0.1 mg / l of 6-benzylaminoadenine, 0.1 mg / l of naphthaleneacetic acid, and 20 g / l of sucrose to the MS medium mother liquor, and then add 20 μmol / l of 1-β-D-ribofuranose-1, 2,4-Triazole was evenly mixed to obtain the lily induction medium, and the shoot tip meristem was inoculated in the lily induction medium for induction, and a large number of clustered buds could be differentiated after 35 days;
[0033] 4) Divide 3 buds into a small piece and inoculate...
Embodiment 3
[0039] The difference from Example 1 is:
[0040] The method for breeding lily bulbs for producing fresh cut flowers, the specific steps are as follows:
[0041] 1) Put commercial bulbs at a constant temperature of 23°C. When the buds grow to 1.5cm, move them to 35°C for 30 days. At this time, the buds are about 5.5cm long;
[0042] 2) After removing the shoot tip for routine disinfection, use a binocular dissecting microscope to take a shoot tip meristem with a size of 0.2 mm on the sterile operating table;
[0043]3) Add 0.5 mg / l of 6-benzylaminoadenine, 0.5 mg / l of naphthaleneacetic acid, and 30 g / l of sucrose to the MS medium mother liquor, and then add 800 μmol / l of 1-β-D-ribofuranose-1, 2,4-Triazole was evenly mixed to obtain the lily induction medium, and the shoot tip meristem was inoculated in the lily induction medium for induction, and a large number of clustered buds could be differentiated after 40 days;
[0044] 4) Divide 4 buds into a small piece and inoculate...
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