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Tissue culture method for Abelia grandiflora

A technology of tissue culture and hexagoniflora, applied in the field of plant tissue culture, can solve the problems of infectious bacteria, low cutting propagation coefficient, low rooting rate of seedlings, etc., and achieves the effects of wide application, solving the problem of rapid reproduction and shortening the period of seedling raising.

Inactive Publication Date: 2017-11-17
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the small size of the seeds of Hexa daflora, it is not easy to harvest, and it is difficult to carry out seed propagation. Its production is usually propagated by cuttings, divisions or layering. However, these propagation methods have a low reproduction coefficient, a long seedling period, and are prone to infection of bacteria and species. Degradation is fast, especially for the mosaic varieties in the large-flowered Hexagram, there are very few introductions, and they are rare in the market. The cutting propagation coefficient of these mosaic varieties is even lower, and the rooting rate is low
[0007] At present, there are very few reports on the tissue culture methods of Hexapia grandis. The maximum coefficient is only 4.9, and its seedling rooting rate is low, only 65% ​​at the maximum, and the whole breeding cycle is 165 days, and the cycle is still long

Method used

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  • Tissue culture method for Abelia grandiflora
  • Tissue culture method for Abelia grandiflora
  • Tissue culture method for Abelia grandiflora

Examples

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Embodiment 1

[0048] A kind of tissue culture method of embodiment 1 a kind of Hexa japonica mosaic variety ' Mrs. Bella (BELLA DONNA) ', comprises the following steps:

[0049] The explants come from the plant of Hexa japonica planted in the nursery of Fengpu District, Shanghai Academy of Agricultural Sciences; the materials are collected from April to June, and the stem segments with axillary buds germinated in that year are selected as the explants.

[0050] Disinfection of explants: Take the 5cm section of the stem with axillary buds of Hexapodia daflora as explants, cut off the excess leaves on the branches, clean the explants with a fine-bristled brush dipped in detergent, and rinse them with running water for 1-2 hours. Disinfect with 70% alcohol for 30-60 seconds on an ultra-clean bench, 2% sodium hypochlorite for 10-15 minutes, add 0.5% Tween-20 solution to the solution, rinse with sterile water 3 times, and set aside.

[0051] Primary culture: Cut the sterilized explants into 0.5-...

Embodiment 2

[0062] Embodiment 2 A kind of tissue culture method of Hexa japonica mosaic variety 'GLOSSY' comprises the following steps:

[0063] The explants come from the plant of Hexa japonica planted in the nursery of Fengpu District, Shanghai Academy of Agricultural Sciences. The materials are collected from April to June, and the stem segments with axillary buds germinated in that year are selected as the explants.

[0064] Disinfection of explants: Take the 5cm section of the stem with axillary buds of Hexapodia daflora as explants, cut off the excess leaves on the branches, clean the explants with a fine-bristled brush dipped in detergent, and rinse them with running water for 1-2 hours. Disinfect with 0.1% raw mercury for 8-12 minutes on an ultra-clean bench, add 0.5% Tween-20 solution into the solution, rinse with sterile water for 3 times, and set aside.

[0065] Primary culture: cut the sterilized explants into 0.5-1cm stems with axillary buds, insert them into WPM medium conta...

Embodiment 3

[0075] Embodiment 3 A kind of tissue culture method of Twist of Lime mosaic variety 'Lime (TWIST OF LIME)' comprises the following steps:

[0076] The explants come from the plant of Helicia daflora planted in the nursery of Fengpu Park, Shanghai Academy of Agricultural Sciences;

[0077] Disinfection of explants: Take the 5-7cm section of the stem with axillary buds of Hexapodia daflora as explants, cut off the excess leaves on the branches, clean the explants with a fine-bristled brush dipped in detergent, and rinse 1-2 with running water Hours, disinfect with 70% alcohol for 30-60 seconds on the ultra-clean bench, 2% sodium hypochlorite for 10-15 minutes, add 0.5% Tween-20 solution to the solution, rinse with sterile water 3 times, and set aside.

[0078] Primary culture: cut the sterilized explants into 0.5-1cm stems with axillary buds, insert them into WPM culture containing 6-benzyladenine (6-BA) 2mg / L and naphthaleneacetic acid (NAA) 0.02mg / L In the base, add sucrose 2...

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Abstract

The invention provides a tissue culture method of Abelia grandiflora. Stem segments of the Abelia grandiflora are taken as explants and subjected to primary culture, proliferation culture and rooting culture, rooted plantlets are obtained and subjected to seedling exercising and transplanting, and Abelia grandiflora seedlings are obtained. According to the tissue culture method, 6-BA and NAA are added to a primary culture medium to promote branches to extend from axillary buds, and proliferation of the stem segments can be promoted when 6-BA and NAA are added to a proliferation culture medium; the rapid rooting function can be realized when IBA and NAA are added to a rooting culture medium. With the adoption of the method for tissue culture of the Abelia grandiflora, the seedling period is greatly shortened, propagation coefficient and rooting rate are increased, the seedling period only needs 90-120 days, the propagation coefficient can reach 6.1, and the rooting rate is 95%-98%.

Description

technical field [0001] The invention belongs to the field of plant tissue culture, and in particular relates to a method for tissue culture of Hexa officinalis. Background technique [0002] Abelia biflora (Abelia biflora) is a plant of the genus Abelia in the family Caprifoliaceae. It is native to Jiangxi, Hunan, Hubei, Sichuan and other places in my country. It is a relatively precious ornamental flowering shrub. Have. Rodaogi is not only good for viewing, but also very adaptable. [0003] Abelia grandiflora (Abelia grandiflora) is a horticultural species that appeared earlier in Abelia grandiflora. It is a dwarf variety of Abelia grandiflora. At present, it has been introduced in China, but the number is very limited. It is a hybrid of glutinous rice sticks (A. chinensis) native to China and A. uniflora (A. uniflora), and has been bred abroad to form several more distinctive horticultural varieties. [0004] It grows fast and has a very beautiful plant shape. The branch...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 关媛李水根苏振洪朱建军李秀芬李记开范晓芬
Owner SHANGHAI ACAD OF AGRI SCI
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