Tissue culturing method for Chinese yam

A tissue culture, culture medium technology, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problem of inability to achieve large-scale industrial production, difficult large-scale industrial production, and difficult to kill surface disinfectants. To achieve the effect of reducing comprehensive production costs, easy disinfection, and improving inoculation efficiency

Inactive Publication Date: 2007-05-09
李锐忠
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Problems solved by technology

No matter the leaves, stems, tubers or spare seeds all grow in the wild field, all kinds of miscellaneous bacteria grow on the surface, and some fungi even grow into the inside of the tissue, making it difficult for surface disinfectants to kill the fungi inside the tissue (disinfectant Too strong toxicity also affects its structural organization) and the material is prone to contamination after inoculation, thus affecting the effectiveness of tissue culture
There are also two obvious defects in the stem segments grown from zero-surplus seedlings: (1) it is difficult to break the dormancy of zero-surplus seeds, and the time cost is large, so it cannot be mass-produced
(2) The explant (shoot tip) is small and the inoculation efficiency is low
Therefore adopting above-mentioned explant can not realize large-scale factory production
[0009] 2. The cultivation process is complicated, the cost is high, and it is difficult to realize large-scale factory production

Method used

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  • Tissue culturing method for Chinese yam

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Embodiment Construction

[0033] A method for tissue culture of yam seedlings includes the following steps:

[0034] (1) Explant-Obtaining of Bud: Obtaining Bud includes the following steps:

[0035] (A) Select robust tubers with no disease and insect pests on the surface, disinfect them with 200 times neogelatin or 0.1% potassium permanganate, spray once every 10 minutes or so, spray 5 times, then rinse with tap water, dry, and then cut into pieces Place a block of 10-12 cm in a ventilated place to let the wound dry;

[0036] (B) After 6 hours, put the sand in the sterilized wet sand to accelerate the germination. Keep the temperature between 20 and 30 ℃. The tuber can be obtained within 20 days. The tuber will grow on the tuber at different times. Each tuber Hundreds of buds can be obtained;

[0037] (2) Disinfection of the buds: After the buds grow out, when the diameter is about 0.6cm, cut them off with a sterilized blade, and cut off the roots, soak them with a small amount of detergent, and then rins...

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Abstract

The tissue culture method of Chinese yam includes obtaining bud block as explant, sterilizing bud block, inoculating, provisional planting in greenhouse and other steps. The present invention has the innovations of using bud block as explant to result in less virus, high inoculation efficiency, stable heritability, etc.; improved tissue culture program including inducing rooting, cutting the base part of the bud seedling, soaking in NAA solution of 50 ml / L concentration, inserting in disinfected fine sand for provisional planting and applying diluted MS solution and nutrients and resulting in decreased comprehensive production cost of tissue culture. The present invention makes it possible to realize large scaled production of excellent tissue culture seedling of Chinese yam.

Description

Technical field: [0001] The invention relates to a method for tissue culture of yam (also called Huaishan, Chinese yam, the same below) seedlings. Background technique: [0002] Yam is a plant of the genus Dioscorea that is used both in food and medicine and has a wide range of industrial uses. It is highly valued in traditional Chinese medicine and is also popular in modern nutrition concepts. The growth cycle of yam is short, usually 150 to 180 days. The planting area is wide. Except Tibet and the Northwest Loess Plateau, it is cultivated in all provinces throughout the country. It is concentrated in Henan, Shanxi, Jiangxi, Guangxi, Jiangsu, Shandong, and Hunan. The global annual demand exceeds 30 million tons, and prices have tended to rise in recent years. [0003] Traditional breeding of yam uses the breeding method of top buds, potato pieces, and spare seeds. These methods are costly and are susceptible to virus damage, resulting in degeneration of species, reduced yield an...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 李锐忠
Owner 李锐忠
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