Peptide medicament series for accelerating medulla hematopoiesis cell proliferation

A technology of bone marrow hematopoietic cells and drugs, applied in the field of biotechnology and medicine, can solve problems such as limited sources, achieve normal cell structure and promote cell proliferation

Inactive Publication Date: 2008-09-24
FUZHOU UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0002] The choice of treatment for anemia needs to be determined according to the cause of the anemia. For acute hemorrhagic anemia, the most common blood transfusion can be used to replenish blood. However, for chronic anemia and anemia caused by bone marrow hematopoietic dysfunction, simple blood transfusion can not be enough. fixed
[0003] Bone marrow transplantation is one of the currently used methods of enriching blood

Method used

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  • Peptide medicament series for accelerating medulla hematopoiesis cell proliferation
  • Peptide medicament series for accelerating medulla hematopoiesis cell proliferation
  • Peptide medicament series for accelerating medulla hematopoiesis cell proliferation

Examples

Experimental program
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Example Embodiment

[0030] The preparation method of the short peptide adopts a chemical synthesis method, that is, a very mature solid-phase peptide synthesis method well known in the art, and either the Boc method or the Fmoc method can be used. The specific method is to couple the protected amino acids to an inert solid phase carrier one by one, and then use a strong acid to cleave the peptide chain from the carrier while removing the side chain protection.

[0031]The main amino acid sequence of the short peptide is the main sequence with fixed amino acid positions, and the interval positions are selected to arrange the corresponding amino acids. For example, the amino acid at the J position has two kinds of Ala or Ile; these two kinds of amino acids can be placed in a single place. 1> At the position of J, no matter which one is selected, the short peptide still has its original biological activity, that is, it promotes the proliferation of bone marrow hematopoietic cells. Similarly, the amino a...

Example Embodiment

[0037] Example 1

[0038] Promote the proliferation of bone marrow nucleated cells in patients with aplastic anemia (synthesize the short peptide derivatives of the present invention, add phosphate groups to serine: Ile-Val-Thr-Serp-Leu-Thr-Lys)

[0039] The bone marrow cells of human aplastic anemia patients are separated from lymphocyte separation fluid to separate mononuclear cells, and planted into 96-well cell plates. 5 The cell density per well is planted, and the short peptides involved in the present invention are added to the cell culture plate at different concentrations, and an equal volume of PBS is used as the control group. The experimental results show that the bone marrow nucleated cells are under the action of the short peptide drugs. , The proliferation rate reached 121% in 3 days.

Example Embodiment

[0040] Example 2

[0041] Promote the morphological examination after the proliferation of bone marrow nucleated cells (synthesize the short peptide fragment involved in the present invention, cut 4 or 5 amino acids in the sequence: Ile-Val-Thr-Asp-Leu or Thr-Asp-Leu-Thr- Lys)

[0042] The culture of bone marrow nucleated cells is cultured in DMEM medium containing 10% fetal bovine serum. 6 After planting the cell density per well into the 24-well cell plate, immediately add the short peptide involved in the present invention to the cell culture plate according to different concentrations, and use an equal volume of PBS as the control group. After 24 hours, the cells are centrifuged to remove Supernatant, fix the cell pellet with 3% glutaraldehyde-1.5% paraformaldehyde for 1 day (4℃), then fix with 1% osmic acid-1.5% potassium ferrocyanide for 1.5 hours, rinse with PBS; 70% alcohol Saturated uranyl acetate dye solution block staining, alcohol-acetone gradient dehydration, epoxy re...

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Abstract

The present invention provides peptide drug series that can promote the proliferation of marrow hematopoietic cells. The main amino acid sequence of the short peptide fragments, analogs, derivants and variants of the short peptide of the peptide drug series is larger than or equal to 70 percent equal to and larger than or equal to 90 percent similar to the main amino acid sequence of the short peptide. The short peptide, nucleotides, short peptide fragments, short peptide analogs, short peptide derivants and short peptide variants are used for the preparation of drugs for treating manifold marrow hematopoietic cell defection diseases, congenital anaemia, aplastic anemia and various hemorrhagic anemia diseases and can be applied to the bone marrow transplantation of leukemia patients, etc., that is, the peptide drug of the present invention can treat pancytopenia caused by hemopoietic stem cell defects.

Description

technical field [0001] The invention belongs to the field of biotechnology and medicine, and more specifically relates to a series of peptide drugs for promoting the proliferation of bone marrow hematopoietic cells. Background technique [0002] The choice of treatment for anemia needs to be determined according to the cause of the anemia. For acute hemorrhagic anemia, the most common blood transfusion can be used to replenish blood. However, for chronic anemia and anemia caused by bone marrow hematopoietic dysfunction, simple blood transfusion can not be enough. fixed. [0003] Bone marrow transplantation is one of the currently used methods of enriching blood. It is generally used in the treatment of patients with aplastic anemia or leukemia. However, because the hematopoietic stem cells used for bone marrow transplantation need to be matched, and the source is extremely limited, it is also limited. For the application of this treatment method, the source of stem cells is...

Claims

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Application Information

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IPC IPC(8): C07K7/06C07K1/00C12N15/11C07H21/04A61K38/08A61K31/7088A61P7/06C12Q1/68G01N33/68
Inventor 李昊饶平凡
Owner FUZHOU UNIVERSITY
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