Application of flavone compound or composition thereof to protection of red cell from injury

A flavonoid compound and composition technology, which is applied in the field of flavonoid compound composition, can solve problems such as difficult to protect red blood cells and no protection of red blood cells, and achieve resistance to high-speed centrifugal shear stress damage, improved recovery rate, and safe and convenient operation Effect

Active Publication Date: 2018-06-29
JINAN UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, these preservation solutions and additives are difficult to protect red blood cells from damage in different pH environments, especially to prevent preventive protection of red blood cells against damage caused by abnormal pH
On the other hand, there is currently no relevant technology and method for protecting red blood cells from centrifugal shear stress during the separation process.

Method used

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  • Application of flavone compound or composition thereof to protection of red cell from injury

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] (1) Take 50 μL of human erythrocyte suspension stored in a blood bank, and incubate with 50 μL of 0.4 mg / mL hydroxysafflor yellow A aqueous solution at 37° C. for 4 hours. After the incubation is completed, wash with physiological saline (0.9% NaCl) or PBS buffer three times (1500r / min, 5min / time) to obtain the incubated erythrocyte sample for use. In the following steps of this embodiment, the incubated erythrocyte samples were resuspended to the original volume with the medium required for the experiment, as the treatment group; the erythrocyte suspension without any incubation treatment was used as the control group.

[0043] (2) Resuspend the erythrocyte treatment group sample and control group sample prepared in step (1) after being incubated with hydroxysafflower yellow A solution in phosphate buffered saline (PBS) without adding functional proteins such as serum albumin. )middle. Then, 30 μL of samples from the treatment group and the control group were dropped ...

Embodiment 2

[0049] Equal volumes of red blood cell samples and whole blood were incubated with licochalcone aqueous solution at a concentration of 0.5 mg / mL, and the specific operation steps were the same as in Example 1.

[0050] Then observe and detect the red blood cell sample and the sample of the control group after being incubated with the licorice chalcone solution in the present embodiment with the similar method and operation observation of embodiment 1 and resuspend respectively in the PBS medium that does not add the functional protein such as serum albumin; Hypoxia H 2 o 2 In the PBS+BSA buffer matrix; the pH value is adjusted to be the situation in the PBS+BSA buffer matrix of 5.0, 6.7, 7.5, 8.5, 8.8, 9.7.

[0051] Observing its morphological structure and measuring its deformability respectively, the results show that more than 85% of red blood cells incubated with licochalcone can maintain normal biconcave shape and good deformability in the above-mentioned medium; In ext...

Embodiment 3

[0054] The rutin aqueous solution with a concentration of 0.5 mg / mL was used to incubate the red blood cell sample and the whole blood in equal volume respectively, and the specific operation steps were the same as in Example 1.

[0055] Then observe and detect with the method similar to Example 1 and the operation, in this embodiment, the erythrocyte samples incubated with rutin solution and the samples of the control group are respectively resuspended in the PBS medium without adding functional proteins such as serum albumin; hypoxia H 2 o 2 In the PBS+BSA buffer matrix; the pH value is adjusted to be the situation in the PBS+BSA buffer matrix of 5.0, 6.7, 7.5, 8.5, 8.8, 9.7.

[0056] Observing its morphological structure and measuring its deformability respectively, it is found that more than 86% of the erythrocytes incubated with rutin can maintain normal biconcave and good deformability in the above-mentioned media in the present embodiment; And 9.7 such an extreme envi...

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Abstract

The invention discloses application of a flavone compound and/or a composition containing the flavone compound to the protection and/or prevention of a red cell from an injury and to the maintenance of the quality of a blood product. According to the application, a human red blood cell can be effectively protected and even preventatively protected, and is enabled to be capable of resisting the damage of acidic, alkaline, osmotic pressure-abnormal, anoxic and peroxidated environments deviating from a normal physiological condition and a shear-stress injury caused under high-centrifugal-force washing action; the red cell is enabled to be capable of maintaining the normal structure and function of the red cell. The invention also discloses a method for protecting and/or preventatively protecting the red cell from being injured and maintaining the quality of the blood product. The method is simple in step and safe and convenient to operate; the used flavone compounds of hydroxysafflor yellow A and the like are a medicinal raw material and a food additive which are nationally approved for use; a complex radix angelicae sinensis injection is also a standard traditional Chinese medicinalset-prescription preparation nationally approved for use, and the application is safe and effective, and has a wide application prospect.

Description

technical field [0001] The invention belongs to the field of biotechnology, and particularly relates to the protection and / or preventive protection of red blood cells from various biochemical and mechanical injuries and the maintenance of the quality of blood products by flavonoids and / or compositions containing said flavonoids application. Background technique [0002] In various medical procedures, it is often necessary to transfuse blood to the patient to save the patient's life. Therefore, blood and its products of various blood types should always be stored in the blood bank for occasional needs. Blood transfusion mainly includes whole blood transfusion or blood component (such as red blood cell) transfusion. In clinical practice, about 80% of blood transfusion is red blood cell transfusion. Whole blood is blood collected from veins mixed with a certain amount of anticoagulant preservation solution, mainly red blood cells (about 40% to 50%) and plasma (about 50% to 60...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K47/22A61K47/10A61K47/46A61K35/14A61P7/08A01N1/02G01N21/84
CPCA01N1/0221A01N1/0226A61K35/14A61K47/10A61K47/22A61K47/46G01N21/84
Inventor 黄耀熊
Owner JINAN UNIVERSITY
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