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Organ preserving liquid

A technology of preservation solution and protective agent, applied in the field of organ preservation solution, can solve the problems of discarding, long-term preservation of organ transplant donors, and low preservation quality, and achieve the effects of good preservation, preservation of activity and function, and strong tolerance

Pending Publication Date: 2019-05-21
HEFEI HUAQI BIOLOGICAL ENG CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] As organ donation has become the main source of organ transplant donors, organ donation cases may appear anytime and anywhere, and the time for organ preservation is calculated in hours, and end-stage patients are always threatened by their lives. Discarded due to long storage time or poor storage quality

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0065] Embodiment 1 A kind of organ preservation solution

[0066] The preparation method of the organ preservation solution in this example: under sterile conditions, take part of the deionized water, first add the substance into the deionized water, mix well and adjust the pH to 7.35-7.45 with hydrochloric acid and sodium hydroxide, and finally dilute to 7.35-7.45. 1000mL, and perform supersaturated oxygen blowing to make the liquid full of oxygen and supersaturated.

[0067] Each 1000mL preservation solution contains the components in the following table:

[0068]

[0069]

Embodiment 2

[0070] Example 2 Detection of Rat Liver Transplantation Donor Preservation Effect (HE Staining)

[0071] 20 male SD rats were divided into two groups, 10 in each group. The liver was separated by aseptic operation. During the separation process, it was washed with preservation solution. The warm ischemia time was less than 30 minutes. The isolated livers of the 10 rats in the first group were treated with UW Liquid static 4 ℃ cold storage, the second group of organ preservation solution described in Example 1 low-temperature cycle mechanical perfusion preservation, perfusion pressure and perfusion volume are physiological volume, organ preservation solution in the process of perfusion preservation immediately after supersaturated oxygen The portal vein and hepatic artery immediately enter the liver.

[0072] After the rat liver was preserved by the method of the present invention for 24 hours, samples were taken immediately for HE staining section histological examination acco...

Embodiment 3

[0073] Example 3 Rat liver preservation experiment (BrdU cell proliferation detection)

[0074] 20 male SD rats were divided into two groups, 10 in each group. The liver was separated by aseptic operation. During the separation process, it was washed with preservation solution. The warm ischemia time was less than 5 minutes. The isolated livers of 10 rats in the first group were treated with UW Liquid static 4 ℃ cold storage, the second group of organ preservation solution described in Example 1 low-temperature cycle mechanical perfusion preservation, perfusion pressure and perfusion volume are physiological volume, organ preservation solution in the process of perfusion preservation immediately after supersaturated oxygen The portal vein and hepatic artery immediately enter the liver. At the beginning of in vitro preservation, BrdU (MedChem Express HY-15910) was added to the preservation solution of each experimental group. The initial concentration of BrdU in the preservat...

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Abstract

The invention provides a method for maintaining the survival and / or function of vascular endothelial cells in the in vitro preservation process and used preserving fluid. The method and the preservingfluid are suitable for storing various organs. The in vitro liver can survive to exceed 24h during the preservation by the organ preserving liquid and in the mechanical filling process; the liver microcirculation structure is complete; no obvious damage exists; the choleresis function is good; the liver synthesis and metabolism functions are good; the in vitro preservation time of the liver is greatly prolonged; the postoperative survival rate of later-period liver transplantation patients is improved.

Description

technical field [0001] The present invention provides a liquid for maintaining the survival and / or function of vascular endothelial cells during in vitro preservation, and in particular provides an organ preservation liquid, which is suitable for in vitro preservation of various organs. It belongs to the field of medical equipment. Background technique [0002] At present, organ transplantation is an irreplaceable treatment for end-stage organ disease, cancer, and acute organ failure. However, there is an extreme shortage of various organ donors in China and the world. In the usual organ transplantation process, the effect of organ preservation directly affects the success or failure of the operation and the postoperative survival of the patient. The preservation solution that has the greatest impact on organ preservation is the preservation solution that is in direct contact with the organ. [0003] Since the advent of the University of Wisconsin solution (UW solution) in...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/0226
Inventor 许莉莉许健健沈何放戴焰赵丽娟卞从文黄德峰
Owner HEFEI HUAQI BIOLOGICAL ENG CO LTD
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