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44 results about "Organ preservation solution" patented technology

Composition For Cold Preservation and Perfusion of Organs

InactiveUS20080187901A1Enhancing an osmotic gradientReduce manufacturing costVertebrate cellsDead animal preservationMachine perfusionReperfusion injury
The current invention provides a new organ preservation solution, suitable for machine perfusion, for maintaining viability of organs, parts of organs and tissues. This solution has been designed to overcome a number of problems associated with hypothermic machine perfusion of donor organs, in particular organs obtained from non-heart-beating donors. The solution prevents or minimizes the adverse affects caused by ischemia, hypoxia, energy and nutrient depletion, acidification, hypothermia and reperfusion injury. The preservation solutions according to the current invention are superior to current state of the art preservation solutions, in particular for preservation and perfusion of organs obtained from non-heart-bearing donors, by supplying increased concentrations and an optimized balance of amino acids, vitamins, anti-oxidants, high molecular weight additives and enhanced buffering capacity. In addition, the preservation solution according to the invention combines optimal physical and chemical properties with the use of readily available, inexpensive and pharmaceutically tested and acceptable compounds, reducing the cost of manufacturing and facilitating medical certification of solutions according to the current invention.
Owner:ORGANOFLUSH

Organ preservative fluid and preparation method thereof

InactiveCN101496512AEffective preservationPrevent ischemia-reperfusion injuryDead animal preservationReperfusion injuryTetramethylpyrazine hydrochloride
The invention relates to a preservation solution for organs, tissues or cells of human bodies or animals. The organ preservation solution comprises sodium citrate, potassium citrate, magnesium sulfate, sodium dihydrogen phosphate, sodium hydroxide, adenosine, arginine, tryptophan, mannitol, tetramethylpyrazine hydrochloride and other components. The organ preservation solution can be used for cooling, lavaging and preserving the organs of the human bodies or the animals, can effectively preserve human in vitro kidneys for 48 hours and animal in vitro kidneys for 72 hours, can prevent ischemia-reperfusion injury, and has great value for clinical application.
Owner:YANGZIJIANG PHARMA GROUP SHANGHAI HAINI PHARMA

Organ preservation solution and method for preparing same

ActiveCN102726366AAvoid damageDisadvantages of Avoiding PrecipitationDead animal preservationPhosphateArginine
The invention relates to an organ preservation solution and a method for preparing the same. The method comprises the following steps of: (1) adopting double buffer pairs of citric acids and phosphates to enable the PH value of a solution to be maintained in a range of 7.5 to 8; (2) adding active ingredients such as adenosine, L-arginine, tryptophan, ligustrazine hydrochloride, reduced glutathione and the like and keep the solution stable; (3) to maintain a hypertonic property of the preservation solution with an osmotic pressure of 350-380 mOsm/L, adopting trehalose and gluconolactone as impermeable substances to effectively prevent cellular edema; and (4) adopting polyethylene glycol with a large molecular weight as a colloidal substance to effectively prevent extracellular space from expanding. Compared with the prior art, the method employs raw materials which are all homemade medicinal components and meet the standards of national pharmacopoeia, and are cheap and free of incompatibility; the organ preservation solution prepared is a colorless and transparent liquid which has stable properties, and can be sterilized by high temperature and high pressure, stored at normal temperature or low temperature, and refrigerated, and thus preparation, transportation, storage and usage of the organ preservation liquid are all very easy and convenient.
Owner:SHANGHAI GENEXT MEDICAL TECH

Organ preserving fluid and its prepn

InactiveCN101019529AEasy to excludeEnhanced uptake of stored calciumDead animal preservationHydroxyethyl starchSucrose
The present invention relates to one kind of organ preserving fluid and its preparation process. Each 1000 ml of the organ preserving fluid contains 5 mol / L concentration NaOH solution 3 ml, hydroxyethyl starch 58.5-71.5 g, potassium lactobionate 37.08-45.32 g, MgSO4.7H2O 1.12-1.38 g, adenosine 1.61-1.97 g, allopurinol 0.13-0.16 g, KH2PO4 3.15-3.85 g, cane sugar 9.23-11.29 g, glutathione 0.92-1.12 g, diltiazem hydrochloride 20 mg, KOH and double distilled water in proper amount. The organ preserving fluid has pH 7.3-7.5. Its preparation process includes setting double distilled water in 800 ml in some container, adding the said components via stirring, regulating pH with KOH, adding double distilled water to 1000 ml, filtering and abacterial preservation at 0-8 deg.c.
Owner:南京兰卫医学检验所有限公司

Organ preservation solution and preparation method thereof

The invention relates to the fields of medicine and biology, in particular to an organ preservation solution and a preparation method thereof. Every 1000mL of the organ preservation solution is prepared from the following components by weight: 3.0-15.0g of sodium citrate, 10.0-30.0g of potassium citrate, 5.0-15.0g of mannitol, 0.5-1.2g of potassium chloride, 0.5-1.8g of magnesium sulfate heptahydrate, 0.5-25.0g of a pH buffer pair, 0.2-25.0g of energy substrate and the balance of water for injection. The organ preservation solution also contains one or more of laminarin, trehalose, laminine and betaine. The organ preservation solution is low in price and efficient, and is suitable for preserving multiple organs.
Owner:YANGZIJIANG PHARMA GROUP SHANGHAI HAINI PHARMA

Organ preserving liquid

The invention provides a method for maintaining the survival and / or function of vascular endothelial cells in the in vitro preservation process and used preserving fluid. The method and the preservingfluid are suitable for storing various organs. The in vitro liver can survive to exceed 24h during the preservation by the organ preserving liquid and in the mechanical filling process; the liver microcirculation structure is complete; no obvious damage exists; the choleresis function is good; the liver synthesis and metabolism functions are good; the in vitro preservation time of the liver is greatly prolonged; the postoperative survival rate of later-period liver transplantation patients is improved.
Owner:HEFEI HUAQI BIOLOGICAL ENG CO LTD

Organ preservation liquid

The invention relates to a (KYL) organ preservation solution. The organ preservation solution is prepared from the following raw materials by weight (dosage g / L): 40 to 45 grams of sucrose, 1 to 3 grams of reducibility glutathione, 3 to 4 grams of adenosine triphosphate, 0.12 to 0.18 gram of ulinastatin, 0.6 to 0.8 gram of magnesium sulfate, 0.5 to 0.8 gram of sodium hydroxide, 2 to 3 grams of potassium dihydrogen phosphate, 2 to 4 grams of disodium hydrogen phosphate, 0.6 to 1 gram of radix salviae miltiorrhizae, 0.6 to 0.9 gram of notoginseng, and 10 to 12 grams of glycine. The organ preservation solution has the advantages that: the organ preservation solution has simple preparation and an equivalent preservation effect compared with the UW solution; the organ preservation solution has a better effect than the UW solution regarding the protection for the hepatocyte functions, the hepar microcirculation and the sinus hepaticus endothelial cells; and the organ preservation solution has low price.
Owner:李立

Cardioplegic solution containing lidocaine and adenosine and preparation method thereof

The invention relates to the technical field of cardioplegic solutions, in particular to a cardioplegic solution containing lidocaine and adenosine and a preparation method thereof. Every 1,000ml of organ preservation solution comprises the following components by weight: 5.0-7.0g of sodium chloride, 2.0-3.0g of potassium chloride, 0.3-0.5g of magnesium sulfate, 0.05-0.1g of calcium chloride, 5-8g of glucose, 0.3-0.6g of lidocaine, 0.1-0.2g of adenosine, 0.01-0.02g of astragaloside, 0.05-0.08g of coenzyme Q10, 0.005-0.01g of tetrandrine, 0.005-0.01g of higenamine, 5-8g of histidine and the balance of double distilled water.
Owner:THE AFFILIATED HOSPITAL OF QINGDAO UNIV

Organic preserving solution and its preparation method

InactiveCN101167450ASlow down biochemical reactionsDelay dissipationDead animal preservationSodium lactateMannitol
The invention belongs to the technology of medical biology and relates to organ preservative fluid and the method for making same. The procedure to prepare 1000ml of the fluid is as follows, tetrahydrobiopterin 1.57mg and diazoxide 6.92mg are dissolved in 1ml of dimethyl sulfoxide to act as mother liquid, CaCl2 of 0.1mol / L is prepared for backup, double distilled water 800ml is charged in a container of 1000ml, KCl 1.12g, MgCl2 2.64g, manna sugar 10.93g, histidine 4.63g, glutathion 0.92g and glutavene 3.74g are sequentially charged and agitated to limpidity, 6.90ml of sodium lactate is charged and agitated to limpidity, CaCl2 2.5ml of 0.1mol / L is charged and agitated to limpidity, and backup liquid and mother liquid are charged and agitated to limpidity. The pH is adjusted to 7.3-7.5, the osmotic pressure is adjusted to 320Osm / L, and the volume of double distilled water is set to 1000ml. The fluid is stored in low temperature of 4 DEG C.
Owner:ZHEJIANG UNIV

Quantitative detection method of acetylcysteine related substances in organ preservation solution

The invention provides a quantitative detection method of acetylcysteine related substances in an organ preservation solution. The quantitative detection method is characterized by comprising the steps of: (1) determination of the limits of three acetylcysteine related substances in a preparation; (2) methodology validation of the detection method and determination of the acetylcysteine related substances in the preparation. The quantitative limits of the three related substances are determined by adopting an HPLC (High Performance Liquid Chromatography) detection method with reference to allowed limit values of active ingredient impurities in the preparation and an existing process of the preparation; the methodology validation is carried out on the detection method in the aspects of specificity, linearity, sample injection recovery rate, repeatability, durability and the like to prove that direct injection detection results of a stock solution of the organ preservation solution A are good. The quantitative detection method abandons an inapplicable self-control quantitative method, can be used for realizing the quantitative determination of the acetylcysteine related substances in the organ preservation solution by using an external standard method for the first time and effectively controlling the quality of the organ preservation solution, and is beneficial to the guidance of process study and the improvement of the product safety.
Owner:HUAREN PHARMACEUTICAL CO LTD

Preparation method of organ preservation solution

ActiveCN102524242ASolve the problem of oxidation decompositionStable in natureDead animal preservationAdenosineAcetylcysteine
The invention provides a preparation method of organ preservation solution, which is characterized by comprising carrying out combination preparation to dextran 40, acetylcysteine and sodium hydroxide, filling, sterilizing, and obtaining product solution A; performing combination preparation to potassium citrate, mannitol, potassium chloride, sodium hydrogen phosphate, sodium dihydrogen phosphate, adenosine and magnesium sulfate, filling, sterilizing, and obtaining product solution B, evenly mixing the solution A and the solution B which are of the same volume, and obtaining the organ preservation solution. First acetylcysteine and sodium hydroxide which are equimolar are dissolved with each other to form sodium acetylcysteine, raw materials are dissolved easily, preparation time is reduced, and the raw materials existing in the form of sodium acetylcysteine are stable in nature. The preparation method solves the problem of oxygenolysis of acetylcysteine in the organ preservation solution in the preparation and storage process, is simple and good in stability.
Owner:HUAREN PHARMACEUTICAL CO LTD

Organ Function Maintaining or Amelorating Solution

It is intended to provide an organ function maintaining or ameliorating solution, which is effective for suppressing the depression of an organ provided for transplantation and an organ preservation method using the organ function maintaining or ameliorating solution.The present invention relates to an organ function maintaining or ameliorating solution for maintaining or ameliorating the functions of an organ provided for organ transplantation, comprising a pharmacologically active substance comprising glycyrrhizic acid, glycyrrhetinic acid or a pharmacologically acceptable salt or derivative thereof in an organ preservation solution. The present invention also relates to a method of preserving an organ for organ transplantation comprising perfusing the organ function maintaining or ameliorating solution to the organ isolated from the donor to cause ischemia and immersing the organ in the organ function maintaining or ameliorating solution during the time until the organ is transplanted into a recipient.
Owner:NAT UNIV CORP TOKYO MEDICAL & DENTAL UNIV +1

Improved Celsior preservation solution for donor heart, and preparation method and application thereof

The invention discloses an improved Celsior preservation solution for a donor heart, and a preparation method and application thereof. The improved Celsior preservation solution for the donor heart comprises potassium chloride, magnesium chloride, calcium chloride, mannitol, histidine, reduced glutathione, monosodium glutamate, sodium lactobionate and linagliptin, with the balance being double-distilled water. The preparation method comprises the following steps: adding the double-distilled water into a container, then successively adding potassium chloride, magnesium chloride and calcium chloride, and carrying out stirring until the color of the obtained mixture becomes clear; then adding mannitol, histidine, reduced glutathione and monosodium glutamate and carrying out stirring until the color of the obtained mixture becomes clear; adding sodium lactobionate and carrying out stirring until the color of the obtained mixture becomes clear; and adjusting the pH value and osmotic pressure value of the obtained solution, fixing the volume of the solution with the double-distilled water and preserving the solution at a low temperature of 4 DEG C. The improved Celsior preservation solution has the advantages of good and long-term organ preservation effect and capacity of promoting recovery of heart functions after reperfusion of the donor heart.
Owner:ZHEJIANG UNIV

Enhancer of survival of transplanted organ

The present invention provides an agent for promoting graft survival which can suppress rejection without use of existing immunosuppressants, an organ preservation solution capable of maintaining the freshness of an organ excised from a donor, and the like. An agent for promoting graft survival or an organ preservation solution is prepared, which comprises 5-aminolevulinic acid (ALA) or a derivative thereof, or a salt of ALA or the derivative and an iron compound as active ingredients. Preferable examples of the ALAs can include ALA and various esters such as methyl ester, ethyl ester, propyl ester, butyl ester, and pentyl ester of ALA, and their hydrochlorides, phosphates, and sulfates. Preferable examples of the iron compound can include sodium ferrous citrate.
Owner:SBI PHARMA CO LTD +1

Transplant cold protection bag

The invention relates to a transplant cold protection bag which is formed by three layers of plastic composite films. A transplanted organ placement bag, a refrigerating bag and a heat insulation bag which are formed by middle films, are mutually closed and are provided with inner films are arranged in a wear-resistant plastic bag of an outer film; the transplant cold protection bag can be used for storing organ preservation solution, transplanted organs, a cool storage material and a heat insulation material which have temperatures of 0 to 4 DEG C; one end of the transplanted organ placement bag, together with the wear-resistant plastic bag, is provided with a transplanted organ taking and storing port formed by two magnets and a sealing material together; and the other end of the transplanted organ placement bag, together with the wear-resistant plastic bag, is provided with a liquid replacement tube with a blockage cap. The transplant cold protection bag disclosed by the invention ensures a low temperature of 0 to 4 DEG C, which is required by transplants in an operation and enables the transplants to have no warm ischemia time; the body temperature of a receptor is prevented from being reduced due to conduction of brine ice gauze and complications are reduced; and the transplants are convenient to move and a touch technology is not adopted. The transplant cold protection bag greatly improves the survival rate of postoperative organs and has important clinical significance and application prospect for storage of the transplants in the organ transplantation and vascular reconstruction process.
Owner:DALIAN MEDICAL UNIVERSITY

Organ preservation solution and liver in-vitro preservation method

InactiveCN113303326AImprove the quality of donated liverReduce donor liver damageDead animal preservationLiver tissueLiver necrosis
The invention is applicable to the technical field of medical treatment, and provides an organ preservation solution and a liver in-vitro preservation method, the organ preservation solution comprises the following components: a UW solution and resveratrol; the combined use of the resveratrol and the UW liquid can reduce liver enzymatic injury markers and improve the liver tissue form, which indicates that the combined use of the resveratrol and the UW liquid can effectively improve the donor liver quality and reduce the donor liver injury; particularly, a remarkable protection effect is achieved in static cold preservation of liver transplantation donor liver; and the problems that an existing organ preserving fluid is difficult to repair the liver of a donor, but due to the limited preserving effect of the organ preserving fluid, many marginal donor livers completely fall into waste livers after a traditional preserving mode is applied, and a large number of precious liver sources are wasted are solved.
Owner:JILIN UNIV FIRST HOSPITAL

Organ preservation liquid, its preparation method and use thereof

The preservative fluid for organ contains soluble TNF receptor and soluble IL-1 receptor or carrier capable of expressing the described receptor. The application of said preservative fluid can effectively reduce host-versus graft and graft versus host reaction in the process of organ transplantation, can prolong survival time of graft and raise survival rate of graft in host body interior, so that it can be effectively used for organ transplantation.
Owner:ZHEJIANG UNIV

Organ preservation liquid containing fluorocarbon emulsifier

The invention discloses an organ preservation liquid containing a fluorocarbon emulsifier. The organ preservation liquid comprises an oxygen carrier, a cell oxygen free radical protection agent, an emulsifier, a surfactant, a stabilizer, a thickener for viscosity regulation, an electrolyte solution system, and a buffer salt solution for pH (potential of hydrogen) regulation. The organ preservationliquid has the advantages that the oxygen carrying capacity is two times higher than the oxygen carrying capacity of common organ preservation liquid, the nutritional matters required by maintainingof metabolism of isolated organ cells are provided, and the organs can be preserved at sub-low temperature and low temperature; the preservation effect of the isolated livers, kidneys and lungs can beimproved, and the preservation quality is better than the preservation quality of HTK solution and UW solution.
Owner:南京麦荻泉生物科技有限公司

Composition and method for ex vivo immunomodulation and/or preservation of organs, methods and use

The present invention provides a composition for modulating MARCH-I, MHC II and / or CD86 in the cells of an ex vivo organ, that is to say an organ taken from a donor for subsequent transplantation in a recipient. In one embodiment, the composition comprises one or more organ preservation solutions in combination with an Hsp. In another embodiment, the composition of the invention comprises Hsp70 and / or one or more synthetic Hsp70-derived peptides. The composition of the invention provides preservation while at the same time reducing rejection of an organ, thus offering many advantages and solving various relevant technical problems: not only preserving the useful life of the organ but also initiating a process in the organ which inhibits acute rejection; lengthening the useful life of the organ in vivo; having local immunomodulating effects in the recipient; having an indirect impact on the quality of life of the transplant recipient by necessitating fewer immunossuppressive medications; inducing the activity of regulatory T cells (which are acknowledged to be immunosuppressors), reducing the expression of MHC molecules in the graft, reducing alloreactive expression in the recipient's lymph nodes, and lengthening the survival of the graft in the recipient.
Owner:巴西教育和援助联盟

Organ preservation solution

Provided is an organ preservation solution for use in transplantation, which is capable of effectively preventing the occurrence of ischemia-reperfusion injury. Prepared is an organ preservation solution comprising a mixture of the following components: (a) potassium chloride, disodium hydrogen phosphate, sodium dihydrogen phosphate, L-ascorbic acid, L-ascorbic acid phosphate ester, and D-glucose; (b) potassium chloride, disodium hydrogen phosphate, sodium dihydrogen phosphate, L-ascorbic acid, L-ascorbic acid phosphate ester, glycine, and D-glucose; (c) potassium chloride, disodium hydrogen phosphate, sodium dihydrogen phosphate, L-ascorbic acid, L-ascorbic acid phosphate ester, L-cysteine, glycine, and D-glucose; or (d) potassium chloride, disodium hydrogen phosphate, sodium dihydrogen phosphate, L-ascorbic acid, L-ascorbic acid phosphate ester, L-cysteine, glycine, an iron compound, and D-glucose.
Owner:SBI PHARMA CO LTD +1

Storage bag special for storing in-vitro broken limbs

The invention discloses a storage bag special for storing in-vitro broken limbs. The storage bag comprises a first outer bag, a second outer bag and an inner bag positioned inside the first outer bagand the second outer bag, the top of the inner bag is provided with an inlet, a cold storage layer is formed between the inner bag and each of the first outer bag and the second outer bag, and an opening for putting in a coolant is formed in the top outer wall of each of the first outer bag and the second outer bag; a temperature sensor is pasted on the inner wall of the inner bag, a temperature displayer is arranged on the outer wall of the first outer bag, a drainage pipe is arranged at the bottom of the first outer bag, and a first sealing strip and a second sealing strip which are used forsealing the inlet are fixed at the top of the first outer bag and the top of the second outer bag respectively. The storage bag is simple in structure; two layers are adopted to store the broken limbs, organ storage liquid in the inner bag ensures storage effect of the broken limbs, and the cold storage layer between the inner bag and the outer bags realizes cold storage of the broken limbs; temperature of cold storage can be monitored in real time to meet maximum needs of broken limb storage, and survival rate of broken limb re-implantation is increased.
Owner:SECOND AFFILIATED HOSPITAL SECOND MILITARY MEDICAL UNIV

Liver transplantation constant-temperature refrigerator convenient to transport

The invention relates to the technical field of medical instruments, and discloses a liver transplantation constant-temperature refrigerator convenient to transfer. The liver transplantation constant-temperature refrigerator convenient to transfer comprises a box body and a box cover; the top of the box body is rotationally connected with the box cover through a hinge; the box cover is buckled with the box body through a buckle; the box body is sequentially provided with an outer shell, an inner shell and a refrigerator body from outside to inside; and the outer shell and the inner shell are of hollow cuboid structures with openings in the upper and lower surfaces. In the process that organ preservation liquid enters a storage box through a liquid discharging pipe, the organ preservation liquid is divided into small liquid drops by a partition net in a temporary storage bag and falls downwards; in the falling process, germs in the preservation liquid can be killed through irradiation of two ultraviolet lamps; and finally, the preservation liquid drops on multiple sealing petals; and after the preservation liquid in the temporary storage bag is accumulated to a certain amount, under the action of gravity, the multiple sealing petals can be flushed open, and the preservation liquid can enter the storage box to be stored, so that the preservation liquid can be continuously used next time.
Owner:王正昕

Ex vivo organ treatment with peg-phospholipid molecules

An organ graft is ex vivo treated by ex vivo infusing a solution comprising PEG-phospholipid molecules into a vascular system of the organ graft. The solution comprising PEG-phospholipid molecules is ex vivo incubated in the vascular system to enable coating of at least a portion of the endothelial lining of the vascular system with the PEG-phospholipid molecules while keeping the organ or the part of the organ submerged in an organ preservation solution comprising PEG-phospholipid molecules. Such an ex vivo treatment of organ grafts with PEG-phospholipid protected the organ grafts against thromboinflammationand reduced blood pressure drops that otherwise occurred when reperfusing the organ graft in the recipient.
Owner:ICOAT MEDICAL AB

Organic preserving solution and its preparation method

The invention belongs to medical biology technology, and relates to an organ preservation solution and its preparation. According to the preparation steps per 1000ml, it comprises: taking 1.57mg of tetrahydrobiopterin and 6.92mg of diazoxide and dissolving them in 1ml of dimethyl sulfoxide As a mother liquor; prepare 0.1mol / L CaCl210ml for later use; add 800ml double distilled water into a 1000ml container, add KCl 1.12g, MgCl22.64g, mannitol 10.93g, histidine 4.63g, glutathione 0.92g, Sodium glutamate 3.74g, stir until clear; add sodium lactate 6.90ml, stir until clear; add 0.1mol / L CaCl22.5ml, stir until clear; add stock solution and mother liquor, stir until clear. The pH was adjusted to 7.3-7.5, the osmotic pressure was adjusted to 320Osm / L, and the volume was adjusted to 1000ml with double distilled water. Store at 4°C for low temperature storage.
Owner:ZHEJIANG UNIV

Composition having compound accelerating phosphorylation of ampk as effective component

InactiveUS20180290979A1Improve survivalEnhances phosphorylation of AMP-activatedOrganic active ingredientsOrganic chemistryCancer cellActive component
Provided is a composition that can be used for an organ preservation solution or perfusate. Alternatively, provided is a composition for prolonging the survival of normal cells and / or a composition for inhibiting the survival of cancer cells. In one or more embodiments, a composition containing, as an active component, a compound that enhances phosphorylation of AMPK (AMP-activated protein kinase). Said composition is one that can prolong the survival of cells (especially, normal cells). Furthermore, said composition is one that can inhibit the survival of cancer cells.
Owner:KYOTO UNIV

Cold organ preservation composition and method of use

InactiveUS9049856B2Dead animal preservationCardiotrophin 1Preservation methods
The present invention relates to a cold organ preservation composition for transplantation comprising a cold organ preservation solution and cardiotrophin-1 or a functionally equivalent variant thereof. The invention also relates to methods and kits for the preparation of said composition, the uses thereof for the cold organ protection and / or preservation for transplantation (particularly for kidney, lung and heart) and also to the cold preservation methods and cold-preserved isolated organs by these methods.
Owner:DIGNA BIOTECH +1
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