Organ preservative fluid and preparation method thereof

A technology of organ preservation solution and ph value, which is applied in the medical field, can solve the problems of not enough to prevent cell acidification, not suitable for general application, high potassium ion content, etc., to achieve outstanding anti-blood reperfusion injury, prevent ischemia-reperfusion injury, Effect of enhancing SOD activity

Inactive Publication Date: 2009-08-05
YANGZIJIANG PHARMA GROUP SHANGHAI HAINI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] However, this preservation solution also has disadvantages: 1. The concentration of magnesium sulfate is high, and it is easy to precipitate at low temperature, and it precipitates in the preservation solution and renal blood vessels to cause tissue damage; 2. It does not contain effective buffer pairs, and the pH value is unstable. Time (more than 24 hours) is not enough to prevent cell acidification when storing the kidney; 3. It contains more biochemical agents and the purity is not enough
Although UW liquid has been widely used in the world, its formula liquid has high viscosity, so the cooling speed is slow; the potassium ion content is too high, improper use will cause cardiac arrest in the operation, and the price is very expensive, so it is not suitable universal application

Method used

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  • Organ preservative fluid and preparation method thereof
  • Organ preservative fluid and preparation method thereof
  • Organ preservative fluid and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0042] formula:

[0043]

[0044] Preparation:

[0045] 1. Concentrated preparation: dissolve potassium citrate, sodium citrate, magnesium sulfate, sodium dihydrogen phosphate, adenosine, arginine, tryptophan, mannitol and sodium hydroxide with 500ml of water for injection, and add Add water for injection to 80% of the total volume, add 0.5 g of medicinal charcoal, stir well, and decarbonize to a clear liquid.

[0046] 2. Dilute formulation: add ligustrazine hydrochloride to the above-mentioned concentrated formulation, add water for injection to the full amount, stir well, and filter the liquid medicine through a 0.45 μm filter membrane.

[0047] 3. Check the pH value and osmotic pressure value of the diluted solution in step 2: the pH value is 7.65, and the osmotic pressure value is 370mOsm / L. Filter the drug solution through a 0.2 μm membrane filter.

[0048] 4. Potting: Put the liquid medicine on the assembly line for potting.

[0049] 5. Sterilization: Sterilize the ...

Embodiment 2

[0051] formula:

[0052]

[0053] Preparation:

[0054] 1. Concentrated preparation: Dissolve potassium citrate, sodium citrate, magnesium sulfate, sodium dihydrogen phosphate, adenosine, arginine, tryptophan, sorbitol and sodium hydroxide with 500ml of water for injection, supplement Add water for injection to 80% of the total volume, add 0.5 g of medicinal charcoal, stir well, and decarbonize to a clear liquid.

[0055] 2. Dilute formulation: add ligustrazine hydrochloride to the above-mentioned concentrated formulation, add water for injection to the full amount, stir well, and filter the liquid medicine through a 0.45 μm filter membrane.

[0056] 3. Check the pH value and osmotic pressure value of the dilute solution in step 2: the pH value is 7.61, and the osmotic pressure value is 366mOsm / L. Filter the drug solution through a 0.2 μm membrane filter.

[0057] 4. Potting: Put the liquid medicine on the assembly line for potting.

[0058] 5. Sterilization: Sterilize ...

Embodiment 3

[0060] formula:

[0061]

[0062] Preparation:

[0063] 1. Concentrated preparation: dissolve potassium citrate, sodium citrate, magnesium sulfate, sodium dihydrogen phosphate, adenosine, arginine, tryptophan, mannitol and sodium hydroxide with 500ml of water for injection, and add Add water for injection to 80% of the total volume, add 0.5 g of medicinal charcoal, stir well, and decarbonize to a clear liquid.

[0064] 2. Dilute formulation: add ligustrazine hydrochloride to the above-mentioned concentrated formulation, add water for injection to the full amount, stir well, and filter the liquid medicine through a 0.45 μm filter membrane.

[0065] 3. Check the pH value and osmotic pressure value of the dilute solution in step 2: the pH value is 7.89, and the osmotic pressure value is 377mOsm / L. Filter the drug solution through a 0.2 μm membrane filter.

[0066] 4. Potting: Put the liquid medicine on the assembly line for potting.

[0067] 5. Sterilization: Sterilize the...

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Abstract

The invention relates to a preservation solution for organs, tissues or cells of human bodies or animals. The organ preservation solution comprises sodium citrate, potassium citrate, magnesium sulfate, sodium dihydrogen phosphate, sodium hydroxide, adenosine, arginine, tryptophan, mannitol, tetramethylpyrazine hydrochloride and other components. The organ preservation solution can be used for cooling, lavaging and preserving the organs of the human bodies or the animals, can effectively preserve human in vitro kidneys for 48 hours and animal in vitro kidneys for 72 hours, can prevent ischemia-reperfusion injury, and has great value for clinical application.

Description

Technical field: [0001] The present invention relates to medicine, in particular to a preservation solution for human or animal organs, tissues or cells, especially a preservation solution for human or animal organs. Background technique: [0002] Organ transplantation is a major advance in human medical science in the 20th century. Since its clinical application in the 1960s, transplantation medicine has developed by leaps and bounds. At present, the total number of transplants in the field of kidney, liver, heart, pancreas and bone marrow transplantation in the world has exceeded 700,000 cases (times), and the longest surviving organ transplant recipient has been nearly half a century. Organ transplantation has become one of the main directions of medical development in the 21st century, and it is an effective cure for end-stage organ failure. However, any clinical organ transplantation must first have a high-quality donor, which is the prerequisite and fundamental guaran...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
Inventor 朱有华甘益民周庆武纪标
Owner YANGZIJIANG PHARMA GROUP SHANGHAI HAINI PHARMA
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