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Organic preserving solution and its preparation method

An organ preservation solution and mannitol technology, applied in the fields of biology and medicine, can solve the problems of organ transplantation obstacles, cannot reduce the re-injury of organ transplantation perfusion, etc., to inhibit acidosis, effectively resist oxygen free radical damage, and prolong survival time. Effect

Inactive Publication Date: 2009-10-07
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, at present, this kind of preservation solution can not reduce the perfusion re-injury in the process of organ transplantation, and there are certain obstacles to further improving the survival rate after organ transplantation.

Method used

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  • Organic preserving solution and its preparation method
  • Organic preserving solution and its preparation method
  • Organic preserving solution and its preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] Preparation of organ preservation solution (take the preparation of 1000ml as an example):

[0023] Preparation of raw materials: mannitol 10.93g; histidine 4.63g; glutathione 0.92g; sodium glutamate 3.74g; sodium lactate 6.90ml; KCl 1.12g; MgCl 2 2.64g; 0.1mol / L CaCl 2 2.5ml; tetrahydrobiopterin 1.57mg; diazoxide 6.92mg; sufficient double distilled water.

[0024] making process:

[0025] Specific steps are as follows:

[0026] Dissolve 1.57mg of tetrahydrobiopterin and 6.92mg of diazoxide in 1ml of dimethyl sulfoxide (DMSO) as mother liquor; prepare 0.1mol / L CaCl 2 10ml for later use; add 800ml double distilled water into >1000ml container, then add KCl 1.12g, MgCl 2 2.64g, mannitol 10.93g, histidine 4.63g, glutathione 0.92g, sodium glutamate 3.74g, stir until the color becomes clear; add sodium lactate 6.90ml, stir until the color becomes clear; add 0.1mol / L CaCl 2 2.5ml, stir until the color becomes clear; add the spare mother liquor, stir until the color ...

Embodiment 2

[0028] Application of Organ Preservation Solution in Langendoff Isolated Rat Heart Perfusion and Cold Preservation

[0029] Subject: Male SD rats (body weight 230-270g)

[0030] Rats were divided into ① control group: isolated hearts were preserved in Celsior cardioplegia solution, n=8; ②DE+BH 4 Group: The isolated heart was preserved in the organ preservation solution provided in Example 1 (hereinafter referred to as "the organ preservation solution"), n=8.

[0031] Implementation process:

[0032]After the rats were weighed, the thoracotomy was taken out, and the heart was quickly transferred and fixed on the Langendoff perfusion device, and conventional retrograde constant pressure perfusion (76mmHg) was performed with modified K-H solution. The temperature was maintained at 37 °C throughout the perfusion period, and the perfusion fluid was filled with 95% O 2 +5%CO 2 saturation. The heel of the pulmonary artery is cut to allow adequate drainage of the coronary reflux ...

Embodiment 3

[0034] The cardiac function index before and after preservation described in embodiment 2 is measured, and the results are as follows:

[0035] Table 1 Changes in left ventricular end-diastolic pressure (LVEDP) after cold storage for 8 hours

[0036]

[0037] * p<0.05 vs control group. After cold storage for 8 hours, compared with pure Celsior solution, this storage solution can significantly inhibit the increase of LVEDP after storage.

[0038] Table 2 Changes of left ventricular developing pressure (LVDP) after cold storage for 8h

[0039]

[0040] ** p<0.05vs control group. After cold storage for 8 hours, the effect of this preservation solution on promoting the recovery of LVDP was significantly better than that of Celsior solution alone.

[0041] Table 3 Changes in the recovery rate of coronary outflow (CF) after cold storage for 8h

[0042]

[0043] #p<0.05 vs control group. After cold storage for 8 hours, compared with pure Celsior solution, this preservati...

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Abstract

The invention belongs to medical biology technology, and relates to an organ preservation solution and its preparation. According to the preparation steps per 1000ml, it comprises: taking 1.57mg of tetrahydrobiopterin and 6.92mg of diazoxide and dissolving them in 1ml of dimethyl sulfoxide As a mother liquor; prepare 0.1mol / L CaCl210ml for later use; add 800ml double distilled water into a 1000ml container, add KCl 1.12g, MgCl22.64g, mannitol 10.93g, histidine 4.63g, glutathione 0.92g, Sodium glutamate 3.74g, stir until clear; add sodium lactate 6.90ml, stir until clear; add 0.1mol / L CaCl22.5ml, stir until clear; add stock solution and mother liquor, stir until clear. The pH was adjusted to 7.3-7.5, the osmotic pressure was adjusted to 320Osm / L, and the volume was adjusted to 1000ml with double distilled water. Store at 4°C for low temperature storage.

Description

technical field [0001] The invention belongs to the technical fields of biology and medicine, relates to organ preservation in organ transplantation technology, and more specifically relates to an organ preservation solution and a preparation method thereof. Background technique [0002] Organ transplantation is a major advance in human medical science in the 20th century. At present, the total number of transplants in various fields in the world has exceeded 700,000 cases (times), and the longest surviving organ transplant recipient has been nearly half a century. Organ preservation is one of the three technical pillars of organ transplantation. In order to improve the long-term survival rate of patients and alleviate the contradiction of the shortage of donor organs, transplant clinics put forward higher requirements for organ preservation technology. [0003] The University of Wisconsin Solution (UW solution), successfully developed by Professor Belzer of the University...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N1/02
Inventor 张晓明王丽娜朱一苗沈岳良郑鸣之
Owner ZHEJIANG UNIV
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