The invention relates to an amphibian isolated heart perfusion device. The device comprises an arterial cannula, a venous cannula, a drip recording electrode and an electrocardio lead electrode, wherein the arterial cannula is formed by combining an arterial cannula trunk, an aorta inserting end and a liquid discharging pipe through a three-way valve; the venous cannula is formed by combining a liquid storage tank, a postcaval vein inserting end and a liquid adding pipe through a three-way valve, and parts of the arterial cannula and the venous cannula are inserted and combined into one. The drip recording electrode comprises a drip recording electrode head, a drip recording electrode rod and a signal input end; the electrocardio lead electrode comprises an electrocardio lead electrode head, an electrocardio lead electrode rod, a signal input end, a heart outer wall liquid adding pipe and a three-way valve. The multifunctional amphibian isolated heart perfusion device is suitable for one person to operate, facilitates perfusion liquid replacement, and is suitable for synchronously and dynamically detecting dynamical changes of the cardiac output, myocardial contraction tension, electrocardiogram and various cardiac function indexes.