27 results about "Isolated heart" patented technology

The invention is applicable to the technical field of heart perfusion devices, and provides an isolated heart perfusion experimental device and an isolated heart perfusion experimental method, blood in an isolated heart can be squeezed out by arranging a fixing mechanism, blood coagulation in the heart is avoided, meanwhile, the heart can be fixed, and the device is convenient to use. Meanwhile, the transverse position, the longitudinal position and the deflection angle of the fixed heart can be adjusted in cooperation with the adjusting mechanism, so that the heart aorta can be aligned with a perfusion conveying pipe, subsequent perfusion of the heart is facilitated, the heart aorta can be expanded by arranging the injection mechanism, and the injection efficiency is improved. Therefore, the conveying pipe can be conveniently inserted into the aorta, the efficiency of inserting the conveying pipe into the heart aorta can be improved, the inner wall of the heart aorta can be adsorbed and fixed to the outer portion of the conveying pipe in cooperation with the limiting structure at the end of the conveying pipe, and therefore the heart aorta can be ligated without additionally arranging a binding wire; and the perfusion efficiency of the device is further improved.

The disclosure provides, in various embodiments, systems, devices and methods relating to ex-vivo organ care. In certain embodiments, the disclosure relates to maintaining an organ ex-vivo at near-physiologic conditions. The present application describes, for example, a method for using lactate measurement in the arterial and the venous blood lines of the Organ Care System Heart perfusion device to evaluate, for example, the: 1) overall perfusion status of an isolated heart; 2) metabolic status of an isolated heart; and 3) overall vascular patency of an isolated donor heart. This aspect of the present disclosure may use, for example, the property of myocardial cell's unique ability to produce / generate lactate when they are starved for oxygen and metabolize / utilize lactate for energy production when they are well perfused with oxygen.

The invention relates to an in-vitro left heart near physiological environment simulation system and method based on an isolated heart, and relates to the technical field of medical instruments. The system comprises a simulation assembly and an external circulation unit. A shell of the simulation assembly is transparent and used for containing the isolated heart, a top cover is provided with an inflow channel, an outflow channel and an observation window, and a reciprocating pump changes a containing cavity through an isolating membrane to hydraulically drive the heart. The external circulation unit is provided with a resistance valve, a compliance assembly and the like. The method comprises the steps of treating the isolated heart, connecting pipelines, assembling parts, adjusting liquid flow and pressure and the like. The left heart dynamic near-physiological simulation of the isolated heart can be realized, the valve movement can be conveniently observed, and the heart movement can be observed from different angles. More importantly, a near-physiological in-vitro testing environment can be provided for cardiovascular implantation and intervention medical instruments (such as an intervention type mitral valve, a mitral valve forming ring and a ventricular septum plugging device), and a way is provided for researching the influence of the near-physiological in-vitro testing environment on valve movement and left heart movement.

The isolated heart storage and transfer device comprises a base, moving wheels are arranged on the base, a protective frame is arranged above the base, buffer strips are fixedly connected to the periphery of the protective frame, fixing rods are fixedly connected between the protective frame and the base, and a transfer box is arranged on the inner side of the protective frame; a vertical buffer mechanism is arranged at the top of the base; a horizontal buffer mechanism is arranged in the middle of the vertical buffer mechanism; the horizontal buffering mechanism comprises a horizontal moving structure and a reset buffering structure. By arranging the vertical buffer mechanism, the heart in the transfer box can be prevented from being affected by impact from the bottom of the device; by arranging the horizontal buffer mechanism, the heart in the transfer box can be prevented from being affected by impact in the horizontal direction of the device.

The invention discloses a zebra fish heart electrophysiology detection method and application thereof, and belongs to the technical field of biological tissue dielectric property analysis. The method is mainly carried out by using a Taiwan solution especially suitable for keeping the vitality of the zebra fish heart and a dye solution special for the isolated heart of the zebra fish. The Taiwan solution is an isotonic ion solution, and various ions dissolved in the Taiwan solution can ensure ion exchange of the zebra fish heart, so that the zebra fish heart is prevented from being inactivated due to over-infiltration or water loss conditions and the like. The dye liquor prepared on the basis of the Taiwan's solution can ensure the activity of the zebra fish heart in the long-time dyeing process. The dye liquor is a combination of low-concentration and high-concentration calcium-sensitive infection solutions, and the use sequence is that the high concentration is performed firstly and then the low concentration is performed. The method can meet the requirements of various data required in electrophysiology related literatures, especially can reflect the change condition of calcium signals in cells, and also has the advantages of low cost, short experimental period and the like.

A Windkessel simulation apparatus includes a flexible fluid container that cyclically fills with a perfusate fluid, such as blood, and helps deliver the perfusate fluid to a heart, by means of an elastic reactive squeezing mechanism that applies compressive force proportional to the pressure in the flexible fluid container. The apparatus acts as a hydraulic capacitor, similarly to how arteries act in the body-inflating during systole, and squeezing during diastole. In this way, the apparatus may help simulate a natural circulatory system for an organ, such as an ex vivo heart.

An integrated bubble trap device for a working heart system includes a body having an internal cavity configured to be partially filled with a liquid, an inlet configured to be in fluid communication with a liquid reservoir, an outlet configured to connect to a left atrium of an excised heart of a test specimen, and a syringe port configured to connect the internal cavity of the body with a syringe. An aperture at the bottom of the body is configured to connect to a pressure sensor. The integrated bubble trap device may further include a catheter extension configured to connect the liquid reservoir to the inlet adapter of the integrated bubble trap device and an adjustable clamp to hold the liquid reservoir in a position. In a method of use, the integrated bubble trap device removes bubbles from a perfusion buffer.

The application discloses an ex vivo heart perfusion system and a perfusion control method, wherein the perfusion system comprises: an organ bin, which is externally provided with a constant temperature heating film for controlling the temperature in the organ bin to be a first preset temperature threshold; a liquid storage container for containing a perfusion medium, an outlet end of the liquid storage container being connected to a first active branch vein through a first liquid supply pipe and connected to a pulmonary vein through a second liquid supply pipe, an inlet end of the liquid storage container being connected to a second active branch vein through a first liquid return pipe and connected to a pulmonary artery through a second liquid return pipe; a circulating pump connected to the first liquid supply pipe; an oxygenator connected to the first liquid supply pipe, the oxygenator being connected with a gas supply module for adjusting the gas mixing ratio in the perfusion medium; and an injection pump group for supplementing injection of nutrient substances or therapeutic drugs. The application can effectively prolong the heart supply storage time, reduce the heart supply discard rate, and further improve the utilization rate of the heart supply.

The present invention discloses a component for in vitro near-physiological simulation of an isolated heart, belonging to the field of medical device technology. Its design purpose is to solve the many problems existing in existing in vitro heart simulation devices, simulate the physiological movement of the heart more accurately and simplify the valve movement observation process. The component includes a transparent shell, a top cover and a reciprocating pump. The shell has a accommodating chamber for placing the isolated heart, and the top cover has inflow and outflow channels respectively connected to the left atrioventricular orifice and the aortic orifice of the heart. An observation window is provided on the top cover to observe the valve movement. The reciprocating pump changes the hydraulic pressure of the accommodating chamber through an isolation membrane to cause the heart to contract and relax. The component is also provided with multiple pressure measuring interfaces and pressure measuring components, and the shell is polyhedron for easy observation. The component can be used to accurately simulate cardiac movement and is suitable for the evaluation of cardiac implantable medical devices, such as the evaluation of parameters such as the function, biomechanical properties and damage mechanism of interventional artificial valves, etc., providing a reliable in vitro simulation environment for the development and verification of medical devices.

The isolated heart perfusion device comprises a rack, a portal frame is fixed to the top of the rack, a fixing plate is fixed to the bottom of a mounting frame through a support, a clamping mechanism is arranged on the fixing plate, the bottom of an intubation tube is sleeved with an air bag, and an air injection mechanism is arranged on the support. During use, after the intubation tube is inserted into the aortic tube, air can be supplied to the air injection cavity in the intubation tube through the air injection mechanism, and the air injection cavity transmits the air to the air bag located at the bottom of the intubation tube, so that the air bag is inflated to be expanded; therefore, the aortic tube area corresponding to the outer side position of the air bag can be expanded to form an independent bulge, and other positions can be kept to be original, so that the aortic tube can be prevented from slipping off from the intubation tube, the pre-fixation of the aortic tube is further realized, the success rate of intubation is improved, the operation time is shortened, repeated intubation is not needed, and the intubation efficiency is improved. The practicability is improved.

The invention relates to the technical field of medical image processing and artificial intelligence analysis, in particular to a mechanical perfusion heart coronary artery recognition and flow velocity estimation method based on laser speckle imaging, and is suitable for donor heart function evaluation and organ quality screening before heart transplantation. Comprising the following steps: continuously imaging the surface of an isolated heart in a mechanical perfusion state to acquire a dynamic speckle image sequence, inputting the dynamic speckle image sequence into a wavelet enhancement nnU-Net model to generate a coronary artery segmentation mask, and applying the coronary artery segmentation mask to the dynamic speckle image sequence to extract image data only containing a coronary artery region, and then generating a blood flow index map, analyzing the periodic change of the blood flow index in the coronary artery region to construct a blood flow velocity change curve, and estimating the hemodynamic parameters of the coronary artery. According to the application, automatic coronary artery identification and quantitative estimation of the blood flow velocity can be carried out on the mechanically perfused isolated heart, so that rapid and accurate non-invasive evaluation on the coronary artery system of the donor heart before heart transplantation is realized.

The invention discloses an assembly for in-vitro near physiological simulation of an isolated heart, and belongs to the technical field of medical instruments. The design aims to solve a plurality of problems of the existing heart in-vitro simulation device, more accurately simulate the physiological movement of the heart and simplify the valve movement observation process. The assembly comprises a transparent shell, a top cover and a reciprocating pump. The shell is provided with a containing cavity for containing an isolated heart, the top cover is provided with an inflow channel and an outflow channel which are connected with a left atrioventricular opening and an aorta opening of the heart respectively, and the top cover is provided with an observation window for observing valve movement. The reciprocating pump changes hydraulic pressure of the containing cavity through the isolating membrane to enable the heart to contract and relax. The assembly is further provided with a plurality of pressure measuring interfaces and pressure measuring assemblies, and the shell is polyhedral so that observation can be facilitated. The component can be used for accurately simulating heart movement, is suitable for evaluating heart implantation interventional medical instruments, such as evaluating parameters such as functions, biomechanical characteristics, damage mechanisms and the like of instruments such as interventional artificial valves, and provides a reliable in-vitro simulation environment for research, development and verification of the medical instruments.

The application discloses a kind of for preventing or treating post myocardial infarction ventricular arrhythmia pharmaceutical composition, the pharmaceutical composition is by everolimus, ethmolam and fluoxetine, belongs to the field of medicine technology.Immunoblotting proves that everolimus, ethmolam and fluoxetine can inhibit fatty acid oxidation protein.In vitro and in vitro cardiac electrophysiology experiment proves that the pharmaceutical composition can effectively reduce post myocardial infarction ventricular arrhythmia susceptibility.TTC staining proves that the pharmaceutical composition can effectively reduce post myocardial infarction myocardial damage area.Based on the above composition in the regulation of post myocardial infarction ventricular arrhythmia susceptibility, it can be further developed in the clinical application of the disease.

The invention discloses an isolated heart transfer box and a using method thereof. The isolated heart transfer box comprises an outer box, an elastic mechanism, an inner box, a temperature adjusting module and a fixing mechanism. The inner box is arranged in the outer box, the bottom end of the inner box is connected with the inner bottom end of the outer box through the elastic mechanism, the elastic mechanism comprises a movable box, a movable block, a spring, a movable groove, a limiting plate, an ejector rod and other assemblies, under mutual cooperation, the inner box can be pressed, the elastic mechanism drives the inner box to move upwards, and the top end of the inner box extends to the outer top end of the outer box; a fixing mechanism capable of fixing the heart is arranged at the inner end of the inner box and comprises a fixing rod, a rotating sleeve, a linkage mechanism, a turbine, a driving motor and other assemblies, and the fixing rod, the rotating sleeve, the linkage mechanism, the turbine, the driving motor and other assemblies are matched with one another to clamp and fix a container for containing the heart, so that the situation that in the transportation process, the heart is damaged is avoided; heart damage caused by displacement and collision affects activity and normal use of the heart.

The disclosure provides, in various embodiments, systems, devices and methods relating to ex-vivo organ care. In certain embodiments, the disclosure relates to maintaining an organ ex-vivo at near physiologic conditions. In certain embodiments, the disclosure relates to placing an ex-vivo heart in a chamber of a portable organ care system; providing, via a pump, a pulsatile flow of a perfusion fluid to the ex-vivo heart; measuring, with a sensor, one or more physiologic characteristics of the ex-vivo heart while the ex-vivo heart is beating; and operating the pump in response to the one or more physiologic characteristics.

This invention provides a modeling device and method for simulating myocardial ischemia-reperfusion injury in an isolated heart, applicable to the field of data processing. This application aims to model ischemia-reperfusion injury in an isolated heart. First, it collects multi-source core data, including samples, perfusion data, environmental data, and physiological monitoring. Utilizing an isothermal perfusion and oxygen partial pressure regulation system, it completes cardiac rewarming, cannulation, and stable perfusion, simultaneously monitoring key indicators such as heart rate, coronary blood flow, and ventricular pressure. Ischemia and reperfusion parameters are integrated to form a basic perfusion dataset. The dataset is hierarchically structured according to physiological indicators and environmental parameters, constructing ternary association nodes for samples, indicators, and parameters. A dynamic physiological association graph is generated through temporal matching and aggregation. Core features are extracted through injury assessment and temporal prediction, generating high-dimensional vectors. These vectors are imported into an adaptive engine for iterative optimization, combined with deviation visualization to form a basic modeling model, outputting modeling information applicable to simulating ischemia-reperfusion injury in an isolated heart.

The invention discloses an application method of a Mongolian medicine compound preparation in isolated cold preservation of heart, and relates to the technical field of heart preservation, comprising a dose-effect experiment process and an animal experiment process; the dose-effect experiment process comprises the following steps: testing the influence of choerospondium-7 pills with different concentrations on the isolated heart, carrying out multi-channel electric marking on the heart, recording field potential and electric signals under stimulation, and determining the optimal onset concentration; the animal experiment process comprises the following steps: step 1, animal isolated heart modeling and grouping (the isolated heart is stored in UW liquid for 8 hours and stored in UW liquid and Mongolian medicine for 8 hours), step 2, animal isolated heart stimulation and position mapping, and step 3, index recording and data acquisition. The cold preservation liquid can be optimized, damage caused by cold preservation is reduced, the preservation effect of the donor heart is improved, the treatment effect of heart transplantation is improved to the maximum extent, ischemic myocardial damage is reduced to the maximum extent, and the isolated heart function is maintained.

1. Name of the product in this design: Electro-gauge thermometer for blood perfusion cardiac transporter. 2. Purpose of this design: for ambient temperature mechanical perfusion of ex vivo hearts during preservation, transportation and final implantation into patients. 3. The key design features of this product are the combination of shape and pattern. 4. The image or photograph that best illustrates the design's key points: a 3D model.

The present application relates to a kind of left heart near physiological environment simulation system and method based on in vitro heart in vitro, it is related to medical instrument technical field.System includes simulation component and external circulation unit;The shell of simulation component is transparent for placing in vitro heart, top cover has inflow, outflow passage and observation window, and reciprocating pump changes the hydraulic drive heart of containing cavity by isolation membrane;External circulation unit has resistance valve, compliance component etc..Method includes the steps of processing in vitro heart, connecting pipeline, assembling component, adjusting liquid flow and pressure etc..The present application can realize the left heart dynamic near physiological simulation of in vitro heart, it is convenient to observe valve movement, also can observe heart movement from different angles.More importantly, it can provide near physiological in vitro test environment for cardiovascular implantation intervention medical instrument (such as interventional mitral valve, mitral valve forming ring and interventricular septum occluder etc.), and provide the way for studying its influence on valve movement and left heart movement.

The invention discloses an in-vitro heart repair system and method. Relates to the technical field of medical instruments, in particular to an in-vitro heart repair system which comprises a perfusion container used for containing and being connected with a perfused in-vitro heart. The pipeline connecting unit is used for connecting the perfusion container with the common carotid artery and the external jugular vein of a receptor animal to form a complete extracorporeal circulation loop; the circulating power unit is arranged on the pipeline connecting unit and is used for driving blood to circulate between the isolated heart in the perfusion container and the acceptor animal; and the detection unit is used for detecting the isolated heart and determining the state of the isolated heart according to the detection result. The in-vivo physiological internal environment can be replicated, and the myocardial cell repairing efficiency is improved.

The utility model relates to the technical field of isolated heart perfusion, and provides an isolated heart perfusion system which comprises a perfusion liquid container, a perfusion tube and a connector, the first end of the perfusion tube is connected with the perfusion liquid container, and the second end of the perfusion tube is connected with the connector; the connector is used for being inserted into the aorta of the isolated heart, and a groove is formed in the outer wall face of the connector. According to the isolated heart perfusion system, the groove is formed in the outer wall surface of the connector, and after the connector is inserted into the aorta of the isolated heart, the position of the groove can be touched across the aorta to determine the insertion depth of the connector, so that the problem that the insertion depth of the connector into the aorta cannot be guaranteed is solved, and the connector is prevented from being inserted too deep or too shallow.

This invention relates to the field of signal denoising, specifically to a method for denoising detection signals from mechanically perfused isolated hearts based on dual-domain transform. The method first decomposes the original detection signal from the isolated heart under mechanical perfusion conditions into multiple signal components. Based on spectral kurtosis, the signal components characterizing fluid noise are removed. A preliminary denoised signal is reconstructed using the retained signal components. Wavelet decomposition is then performed on the preliminary denoised signal to obtain a wavelet coefficient vector. This vector is then optimized using a constructed loss function to obtain the optimal wavelet coefficient vector for the preliminary denoised signal. Based on the optimal wavelet coefficient vector, the signal is reconstructed to obtain the denoised detection signal. This invention effectively removes noise from the detection signal while better preserving the key morphological features of the signal, thus improving the denoising quality of the isolated heart detection signal under mechanical perfusion conditions.

The present application relates to the technical field of medical devices, in particular to a multi-channel electrophysiological mapping system and method for isolated heart tissue based on a microelectrode array, wherein the system comprises: a microelectrode array for contacting and recording original electrical activity signals of excitable tissue or cells; a pressure detection module for collecting systolic and diastolic pressure of the heart tissue; an electrical signal acquisition module for filtering the original electrical signals transmitted by the microelectrode array in combination with the systolic and diastolic pressure to obtain net electrical activity signals, the electrical signal acquisition module comprising a first adaptive filter and a second adaptive filter, the first adaptive filter being used for motion artifact filtering of the original electrical signals according to the systolic and diastolic pressure of the heart tissue, and the second adaptive filter being used for series mode interference filtering of the original electrical signals. The above-mentioned system innovatively filters in combination with the systolic and diastolic pressure signals, effectively improving the signal-to-noise ratio and quality of the net electrical activity signals.

The invention discloses a pharmaceutical composition for preventing or treating ventricular arrhythmia after myocardial infarction, and belongs to the technical field of medicines. The pharmaceutical composition is prepared from everolimus, etomadectin and fluoxetine. Western blot proves that everolimus, etomadectin and fluoxetine can inhibit fatty acid oxidation protein. In-vitro and in-vitro cardiac electrophysiological experiments prove that the pharmaceutical composition can effectively reduce the ventricular arrhythmia susceptibility after myocardial infarction. TTC dyeing proves that the pharmaceutical composition can effectively reduce the myocardial injury area after myocardial infarction. Based on the regulation effect of the composition on the susceptibility of ventricular arrhythmia after myocardial infarction, the clinical application of the composition to the ventricular arrhythmia after myocardial infarction can be further developed.

The invention discloses an isolated heart low-temperature transportation system and a control method, and relates to the technical field of isolated heart transportation, and the isolated heart low-temperature transportation system comprises an outer box body, a controllable spring type buffer surrounding grating assembly, a membrane type inner box body, a low-temperature maintaining unit and a life maintaining unit. The controllable spring type buffer surrounding grating assembly is adopted to achieve profiling adjustability of the storage space, uniform stress of the isolated heart to be transported stored in the membrane type inner box body in all directions, and secondary buffer of oxygen-containing donor heart perfusate stored in the isolated heart to be transported. Damage of vibration in the transportation process to the heart is well avoided, the low-temperature maintaining unit controls the temperature in the membrane type inner box body based on the semiconductor refrigeration technology, the isolated heart is stored in the low-temperature oxygen-containing perfusate environment, and the life maintaining unit can guarantee that heart perfusate with the set capacity and the set oxygen concentration is kept in the membrane type inner box body all the time; the function of the isolated heart can be guaranteed, and the success rate of heart transplantation after low-temperature transportation is increased as much as possible.

The invention belongs to the technical field of medicines, and discloses a pharmaceutical composition for preventing or treating ventricular arrhythmia after myocardial ischemia-reperfusion, the pharmaceutical composition is composed of diosmin, etomoxazide and fluoxetine, and the mass ratio of diosmin to etomoxazide to fluoxetine is 1: 1: 1. Western blot proves that diosmin, etomagazide and fluoxetine can inhibit fatty acid oxidation protein. In-vitro and in-vitro heart electrophysiological experiments prove that the pharmaceutical composition can effectively reduce the susceptibility of ventricular arrhythmia after myocardial ischemia-reperfusion. TTC staining proves that the pharmaceutical composition can effectively reduce the myocardial injury area after myocardial ischemia reperfusion. Based on the regulation effect of the composition on the susceptibility of ventricular arrhythmia after myocardial ischemia-reperfusion, the clinical application of the composition to the ventricular arrhythmia after myocardial ischemia-reperfusion can be further developed.

This invention discloses a method for extracting interstitial fluid from cardiac tissue, belonging to the field of biomedical technology. Based on the unique characteristics of the heart, a organ closely related to blood circulation, and its differences in tissue physical properties compared to other organs, this invention designs and develops this extraction method, which specifically includes: rinsing isolated cardiac tissue with pre-cooled physiological saline; low-speed centrifugation to remove residual rinsing fluid from the heart; and centrifugation again to obtain interstitial fluid from the cardiac tissue. Comparison of the obtained interstitial fluid with cardiac cell fluid and serum confirms the accuracy and specificity of the extraction scheme of this invention. The centrifugation process of this invention does not damage cells, preserving its unique differences from serum and cardiac cell fluid. The extracted interstitial fluid has a high protein concentration, which can better reflect changes in the cardiac microenvironment, facilitating subsequent related medical and basic research.