In-vitro myocardial perfusion device

Abstract

The invention discloses an isolated heart perfusion device, which comprises a base and a bracket, a water bath tube is fixed on the top of the bracket, a K-H liquid bottle and a mixed gas bottle are arranged inside the water bath tube, and the K-H liquid Both the top of the bottle and the mixed gas cylinder are provided with feeding ports, and the bottom of the K-H liquid bottle and the mixed gas cylinder is connected with a spiral mixer through a plexiglass tube, and the spiral mixer is connected with a peristaltic pump through a glass tube. The bottom of the pump is connected with a perfusate casing, and the glass tube between the peristaltic pump and the perfusate casing is connected with an inclined tube, and the inclined tube is connected with a 20ml syringe, and a stop screw is arranged on the 20ml syringe. The bottom of the perfusate sleeve is provided with a 20G drip head, and the bottom of the 20G drip head is provided with a perfusion dish. The perfusion dish is connected to a 100ml syringe through a rubber hose, and the 100ml syringe is installed on the left end of the bracket. A transducer is arranged on the left side of the base, and the transducer is connected with a rubber hose through a glass tube.

Description

technical field [0001] The invention relates to the technical field of experimental equipment, in particular to an isolated heart perfusion device. Background technique [0002] Retrograde perfusion of isolated mammalian heart aorta (Langendorff method) is still an important technical method in the field of cardiovascular research, and its content has gradually been extended to transgenic mouse models associated with the cardiovascular system. The Langendorff method has two basic perfusion methods: constant pressure and constant current. The two methods have their own advantages and disadvantages and application ranges, and the experimental devices are also different. Regardless of the constant pressure and constant flow perfusion methods, glass tubes are required for constant temperature water circulation in the installation structure. It is necessary to consider the heat loss during the flow process. The longer the pipeline, the faster the heat loss, the longer the insula...

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Problems solved by technology

The two methods have their own advantages and disadvantages and application ranges, and the experimental devices are also different. Regardless of the constant pressure and constant flow perfusion methods, glass tubes are required for constant temperature water circulation in the installation structure. It is necessary to consider the heat loss during the flow process. The longer the pipeline, the faster the heat loss, the longer the insulation path of the constant water bath is required, and the temperature change affects the accuracy of the experimental data. At the same time, the existing constant current and constant pressure The perfusion device lacks a corresponding auxiliary liquid adding device. When the auxiliary operation is performed at the same time as the experiment, the original experimental process needs to be stopped, which affects the accuracy of the experiment, and because there are many steps in the experiment, the complicated pipeline connection is easy to cause experimental accident

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