88 results about "Rat myocardium" patented technology

The invention relates to a method for extracting phenolic glycoside compounds from semen lepidii and application thereof, which can effectively obtain the new function of the semen lepidii. The method adopts the technical scheme that the molecular formula of a semen lepidii new glycoside A is C18H24O12[M+Na]<+>m/z 455.1196; the molecular formula of a semen lepidii new glycoside B is C18H24O12[M+Na]<+>m/z 455.1202. The method has the advantages that two new phenolic glycoside compounds are extracted from a water extract of the semen lepidii, namely the semen lepidii new glycoside A and the semen lepidii new glycoside B; by adopting the two new phenolic glycoside compounds, the cell survival rate of the injured hydrogen peroxide-induced rat myocardial cell H9c2 is obviously improved; the function of protecting the myocardial cell is realized, and a new path is provided for preparation of myocardial protection drugs.

The invention discloses an isolation and culture method for primary mice or rat cardiac muscle cells. The isolation and culture method employs specially-prepared compound enzyme for long-term digestion, so the obtained cells are large in gross quantity and high in both isolation degree and motility rate; due to different acquisition manners, pollution and influence of residual blood in a heart are eradicated and the probability of contamination of the acquired cardiac muscle cells by bacteria, fungi and other cells is low; and the method can realize subculturing, so a good cell culture scheme is provided for related research based on culture of cardiac muscles.

The invention relates to the field of medical technology, which discloses a new usage of a petasites tricholobus franch extract and the contained bakkenolide compound in the preparation of drugs for prevention and treatment for cardiovascular and cerebrovascular diseases. The biological activity tests show that, the petasites tricholobus franch extract and the contained bakkenolide compound can significantly reduce the encephalic necrosis percentage of the rats with local cerebral ischemia, improve the behavior score of the ischemic rats and alleviate volume of brain edema and cerebral infarction of the rats with ischemia reperfusion injury, thus prompting that the petasites tricholobus franch extract and the contained bakkenolide compound have significant protective effect on the cerebral ischemia injury. The petasites tricholobus franch extract and the contained bakkenolide compound can further significantly reduce the J-point displacement caused by ISO and the LDH level in plasma, significantly reduce the scope of myocardial ischemic myocardial infarction of the rats caused by coronary artery ligation and lower the LDH level in the plasma of the rats with the myocardial ischemia. As the petasites tricholobus franch extract and the contained bakkenolide compound have good effects on the prevention and the treatment for heart and brain ischemic diseases aspects, the petasitestricholobus franch extract and the contained bakkenolide compound can be used in the preparation of drugs for prevention and treatment for cardiovascular and cerebrovascular diseases, including coronary heart disease, cerebral ischemia, cerebral infarction (stroke), myocardial infarction and so on.

The invention discloses RNA and application of RNA in diseases of a cardiovascular system. According to RNA, the nucleotide sequence of RNA is a sequence 3 in a sequence table. The invention further provides a gene,encoding RNA. The nucleotide sequence of the gene of RNA is a sequence 4 in the sequence table. The experiment of the invention shows that RNA and the application of RNA in the diseases of the cardiovascular system find that by an induction of isoproterenol (ISO), RNAi method Knock down IHP-55 is used in neonatal rat cardiac fibroblasts to obviously promote proliferation of cardiac fibroblasts; ISO induced activation and kinase activity of P38MAPK can be enhanced in the Knock-down HIP-55 cardiac fibroblasts; and HIP-55 is a new protein which has a protective effect on cardiac fibrosis, and provides a new target for clinical diagnosis, treatment and new medicament development.

The invention relates to a high-purity polyphyllin VI preparation method and an application, and belongs to the field of Chinese herbal medicine extraction and separation. The method includes the specific steps: roughly crushing Chinese paris rhizome or wake robin medicinal materials, performing ethanol extraction; sequentially performing macroporous adsorption resin, silica gel and reverse-phase material column chromatography on extracting solution; taking ethyl acetate, ethanol and water as eluting agents and filtering, washing and drying sedimentation portions after recovering solvents by eluent to obtain high-purity polyphyllin VI. The method has the advantages that the operation steps are easily repeated, conditions are easily controlled and amplified, the solvents are low in toxicity and easy to recover, and the like. The obtained polyphyllin VI is basically free from organic solvent residues, and the purity of the polyphyllin VI reaches 98% or more. After pharmacodynamical research of the influence of mouse normal pressure hypoxia tolerance, mouse brain ischemia caused by common carotid artery ligation, rat myocardium injury caused by ischemia reperfusion, myocardial infarction caused by rat isolated heart myocardial ischemia and the like, the polyphyllin VI serving as a final product is good in effect and can be used for developing products for treating cardiovascular and cerebrovascular diseases.

The invention discloses a medicine compound for treating myocardial ischemia reperfusion of a rat, belonging to the technical field of the medicine compound for preventing and controlling myocardial infarction. The medicine compound for treating myocardial ischemia reperfusion of the rat comprises the following ingredients: 5-15 parts of astragalus extract, 5-10 parts of salvia miltiorrhiza, 2-5 parts of carthamus tinctorius, 1-3 parts of rhodiola rosea, 0.25-0.75 part of procyanidine, 0.25-0.75 part of adiponectin and 1-2 parts of puerarin. According to the medicine compound for treating myocardial ischemia reperfusion of the rat and an application method thereof provided by the invention, the myocardial ischemia reperfusion injury can be obviously relieved; procyanidine which has the effect of relieving inflammation and oxidative stress is added into the medicine compound, so that the oxidation resistance of a body can be promoted; adiponectin has the functions of regulating glucolipid metabolism, resisting inflammation and resisting atherosclerosis; the medicine compound has the function of protecting rat myocardium; the medicine compound can greatly reduce the apoptosis rate of the cells and protect the complete form of the cells.

The invention discloses a screening method of tsRNA related to myocardial ischemia reperfusion. The method is characterized by comprising the following steps of building a rat myocardial ischemia reperfusion model, and screening out differentially expressed tsRNA in a myocardial ischemia reperfusion process by adopting a sequencing method; and building an in-vivo rat myocardial ischemia reperfusion model and an in-vitro myocardial cell hypoxia reoxygenation model, and adopting real-time quantitative PCR for sequencing verification. According to the scheme, the tsRNA participates in the myocardial ischemia reperfusion injury process, and an application of the tsRNA in the aspect of myocardial ischemia reperfusion injury treatment is provided, so that a new idea is provided for people to research a pathophysiology process of myocardial ischemia reperfusion injury, and a new molecular basis or a treatment target is provided for prevention and treatment of myocardial ischemia reperfusion injury.

The invention provides an adult rat cardiac muscle cell separation method which comprises the steps: (a) preheating a perfusion system and fully cleaning a perfusion pipeline; (b) sequentially injecting heparin sodium and pentobarbital sodium into the abdominal cavity of a rat; (c) quickly opening the chest of the rat to expose the heart, taking the lower heart from the aortic root, putting into aperfusion solution, appropriately cleaning surrounding tissues and ligaturing the aorta on a perfusion needle; (d) connecting the intubated heart with the perfusion system and perfusing the heart bya perfusion solution without Ca<2+>; (e) continuously perfusing the heart by a mixed digestive enzyme solution; (f) taking the perfused heart, putting into a transfer solution, removing the atrium andgreat vessels, shearing the ventricle into small pieces, utilizing a suction pipe to blow and beat tissues into monodispersion cells, utilizing a filter screen to filter cell suspension and standing;(g) restoring Ca<2+> in a gradient mode; (h) precipitating cells after resuspending a complete medium to restore Ca<2+> and inoculating into a coated cell culture bottle to be cultured. By means of the adult rat cardiac muscle cell separation method disclosed by the invention, adult rat cardiac muscle cells which have high quality, high yield and long survival time can be obtained.

The invention relates to the field of traditional Chinese medicine, and discloses application of phenethyl alcohol glycoside monomer compounds savatiside E and savatiside A in preparing a medicine for preventing and treating myocardial ischemia and hypoxia. According to the invention, the ability of the savatiside E and savatiside A in clearing oxygen radicals is measured by a DPPH method, and the result indicates that the oxygen radicals can be cleared in a dose-dependent mode. The H2O2 and Na2S2O3-caused myocardial cell damage model is adopted to detect the influence on survival rate of the H2O2 and Na2S2O3-caused rat myocardial cell oxidative damage, and the result indicates that the survival rate of myocardial cells can be improved in a dose-dependent mode to suppress peroxidation damage. Moreover, the in-vivo experiments also indicate that the savatiside E and savatiside A can reduce the area of the coronary artery ligation-caused rat myocardial infarction.

The invention relates to a method used for preparing a compound or a biodrug capable of enhancing CNPase activity and used for treating heart disease. According to the invention, chemical methods involve up-regulating the expression and activity of CNPase by using kaurane compounds; Biological therapeutic agents relate to the preparation of CNPase enzymes expressed by using recombinant adeno-associated viruses and therapies of local intervention; and in a rat myocardial hypertrophy and heart failure model, the method can effectively improve myocardial hypertrophy and myocardial remodeling, inhibit myocardial hypertrophy and fibrosis increase and increase cardiac functions.

A Chinese medicine for treating the acute or chronic myocardial ischemia diseases is prepared from 4 Chinese-medicinal materials: notoginseng, Chuan-xiong rhizome, dalbergia wood and valeriana officinalis. Its advantages are quickly taking its high curative effect (2-3 min), low dosage and no toxic by-effect.

The invention belongs to the technical field of medicine and relates to ziziphora bungeana dripping pills, a production method thereof and an application as a cardiovascular medicament. The ziziphora bungeana dripping pills are characterized by containing aromatic ziziphora bungeana total flavonoids and water-soluble pharmaceutical matrix, and the ratio of the water-soluble pharmaceutical matrix to the aromatic ziziphora bungeana total flavonoids in parts by weight is 1: 1 to 5: 1. The pharmacodynamic test result of the obtained ziziphora bungeana dripping pills shows that the ziziphora bungeana dripping pills have a protecting effect on myocardial ischemic injuries of rats, the anti-myocardial ischemia effect is greatly improved in comparison with the raw pharmaceutical material of the aromatic ziziphora bungeana total flavonoids, and the bioavailability and the clinical efficacy are improved. The obtained ziziphora bungeana dripping pills are convenient to use and good in patient compliance, and the production method is easy for industrialization.

The invention relates to the technical field of natural medicine, and particularly discloses an effective exocarpium part and an application of the effective exocarpium part to preparing of medicine for treating diabetic cardiomyopathy. A preparing method of the effective exocarpium part includes the steps that S1, extraction is carried out through an organic solvent for exocarpium, and organic solvent extract is obtained; S2, the organic solvent extract is fed to a macroreticular resin column, elution is carried out through ethyl alcohol with the volume fraction of 10% to 30% for purification, then elution is carried out through ethyl alcohol with the volume fraction of 50% to 70%, and the ethyl alcohol elution part of 50% to 70% is collected; S3, effective ingredients of the ethyl alcohol elution part of 50% to 70% obtained in the step S2 are gathered in a silica gelcolumn chromatography mode, and the effective exocarpium part is obtained. By means of the effective exocarpium part, the content of rat-myocardium NF-kappaB protein can be remarkably reduced, and the effective exocarpium part can be used for treating diabetic cardiomyopathy.

The invention discloses a construction method of an SD rat myocardial ischemia model, and relates to the technical field of medical tests. The construction method of the SD rat myocardial ischemia model comprises the following steps: anesthetizing a rat; cutting the neck skin of the rat open, separating neck muscles until exposing the trachea, and inserting a trachea cannula needle into the trachea of the rat to be connected with a breathing machine; conducting unhairing and skin preparation on the left chest of the rat, separating shallow chest muscles and deep chest muscles, forming an incision between a third rib and a fourth rib, pre-burying a suture line in the muscles between the third rib and the fourth rib, and ligating the left anterior descending branch of the heart through the suture line; suturing the thoracic cavity of the rat by using the suture line, and exhausting the thoracic cavity of the rat by using an injector connected with a soft silica gel catheter; and pulling out the tracheal intubation needle, and suturing layer by layer. According to the construction method of the SD rat myocardial ischemia model, the success rate of rat myocardial ischemia model construction is increased, the operation difficulty of model construction is reduced, and the operation time of model construction is shortened.

The invention relates to a method for establishing an insulin resistance H9c2 cell model by induction of sodium oleate. The invention can effectively solve the problems that an existing insulin resistance cell model is difficult to establish, the modeling rate is low when high-concentration glucose is adopted to induce insulin resistance, and insulin has great instability and is very difficult tostore as a hormone substance. The technical scheme is as follows: 1) performing cell culture; 2) screening a sodium oleate working concentration by a glucose consumption experiment; and 3) carrying out a process of In-cell Western. According to the invention, sodium oleate is selected to induce rat myocardial cell strains, and the insulin resistance model is successfully established. The fact thatsodium oleate can induce cell insulin resistance is verified for the first time, an induction mechanism of sodium oleate is determined, a new application of sodium oleate is opened up, and the methodis an innovation in inducing establishment of an insulin resistance model by sodium oleate.

The invention discloses a pharmaceutical application of BML-284. The application refers to the application of the BML-284 or the pharmaceutically acceptable salt thereof in the preparation of drugs for promoting the proliferation of myocardial cells. Experiments prove that the BML-284 can promote the proliferation of myocardial cells of rats. Therefore, the BML-284 can be used in the related fields of preparation of drugs for promoting myocardial cell proliferation, drugs for treating or preventing heart diseases and the like, and a new drug and a new treatment thought are provided for treating or preventing heart diseases such as myocardial infarction.