Isolated heart perfusion experiment device and isolated heart perfusion experiment method

Abstract

The invention discloses an isolated heart perfusion experiment device. The isolated heart perfusion experiment device comprises a heart perfusion device with a perfusion catheter and a remaining needle. The remaining needle comprises a puncture needle and a catheter of which two ends are opened. The rear end of the catheter is fixedly provided with a fixing connector which is used for detachably connecting the liquid outlet end of the perfusion catheter. The fixing connector is provided with a port communicated with a cavity of the catheter. The front end of the puncture needle successively passes through the port and the cavity of the catheter. The isolated heart perfusion experiment device is used, so that a heart taken from a laboratory mice body can be conveniently and rapidly suspended on a heart perfusion device by an operator, and the activity of the taken heart is guaranteed. The invention further discloses an isolated heart perfusion experiment method using the isolated heartperfusion experiment device.

Description

technical field [0001] The invention relates to an isolated heart perfusion experiment, in particular to an isolated heart perfusion experiment device and an isolated heart perfusion experiment method. Background technique [0002] Isolated heart perfusion experiments are widely used in physiological, pathophysiological and pharmacological research. The experimental method of the isolated heart perfusion experiment is to take out the heart of the experimental animal first, then hang the isolated heart (that is, the extracted heart) on the cardiac perfusion device, and perfuse the heart with perfusate through the cardiac perfusion device. [0003] However, in the current isolated heart perfusion experiment, the operation method of hanging the isolated heart on the cardiac perfusion device is to first find the aorta of the isolated heart, and then move the isolated heart so that the perfusion catheter located in the cardiac perfusion device The outlet port was inserted into t...

AI Technical Summary

Problems solved by technology

If the experimental animal is a mouse, it is very difficult to suspend the isolated heart to the cardiac perfusion device, because the aorta of the smaller experimental animal is smaller, and the operator needs to use a stereo microscope to find the aorta , and the slight shaking of the hands will lead to the difficulty of accurately aligning the outlet end of the perfusion catheter with the aorta, so that the operation difficulty of inserting the outlet end of the perfusion catheter into the aorta of the isolated heart will increase, and the existing perfusion catheter The outlet of the perfusion catheter is a hard structure, and it is easy for the outlet of the perfusion catheter to be inserted too deeply, causing the outlet of the perfusion catheter to damage the isolated heart, and even the perfusion catheter may directly puncture the isolated heart

Therefore, for isolated heart perfusion experiments on mice, since the operation of hanging the isolated heart on the cardiac perfusion device is very difficult, if the operator is not skilled in operation, the operation time of hanging the isolated heart on the cardiac perfusion device will be reduced If it is too long, it will eventually lead to a decrease in the activity of the isolated heart, which will lead to the failure of the experiment or the decrease in the accuracy of the experimental results.

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