Extraction method and application of icariside I in Korean epimedium herb leaf

A technology of icariin and epimedium leaves, which is applied in chemical instruments and methods, medical preparations containing active ingredients, pharmaceutical formulas, etc., and can solve the problems of slow research progress, time-consuming separation process, and difficulties in separation and extraction, etc. problems, to achieve the effect of easy quality control, avoiding low yield, and simple and fast method

Inactive Publication Date: 2010-12-01
JILIN UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The separation and purification of flavonoids by column chromatography has the disadvantage of low yield due to irreversible adsorption, and the separation process is very time-consuming and labor-intensive, which is not conducive to the large-scale separation and extraction of flavonoids
Therefore, although the research on various flavonoids in Epimedium is very urgent, the research progress is very slow due to the difficulty of separation and extraction.

Method used

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  • Extraction method and application of icariside I in Korean epimedium herb leaf
  • Extraction method and application of icariside I in Korean epimedium herb leaf
  • Extraction method and application of icariside I in Korean epimedium herb leaf

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Weigh 5 grams of Epimedium herba leaves, reflux with 75mL volume concentration of 70% ethanol for 4 hours, filter out the ethanol extract, and use 50mL volume concentration of 70% ethanol to reflux extract for 2 hours, filter out Ethanol extract, combined two filtrates, and fixedly dissolved the filtrate to 200mL. Precisely measure 0.2mL of the filtrate to be dissolved in a 25mL volumetric flask, and measure the absorbance value at 270nm to be 0.730; take 10uL of the solution to measure its HPLC chromatogram, and the HPLC chromatogram results are shown in the attached figure 1 .

Embodiment 2

[0032] Take 2.30mg of icariin standard substance in a 25mL volumetric flask, and dissolve it with methanol to obtain a standard solution of icariin with a concentration of 0.092mg / mL. Ultraviolet scanning was performed at 200-600 nm, and 270 nm was the maximum absorbance value of icariin. see attached results figure 2 .

Embodiment 3

[0034] According to Example 2, a standard solution of icariin with a concentration of 0.092 mg / mL was prepared. Take 1.0mL, 1.5mL, 2.0mL, 2.5mL, and 3.0mL of this standard solution in 5mL volumetric flasks, and dissolve them in methanol to prepare concentrations of 0.0184mg / mL, 0.0276mg / mL, 0.0368mg / mL, 0.0460mg / mL, 0.0552mg / mL solution, measure the absorbance value. Draw a standard curve of icariin concentration versus absorbance. According to the standard curve, it can be concluded that the extraction rate of the total flavonoids of Epimedium korean in Example 1 can reach 12%. see attached results image 3 .

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Abstract

The invention relates to an extraction method and application of icariside I in Korean epimedium herb leaves. The extraction method not only can avoid low yield caused by the irreversible adsorption of a chromatographic column, but also can extract a large amount of high-purity icariside I. In the extraction method, content measurement is carried out by a high efficiency liquid chromatography, the quality extraction rate of the icariside I is more than 60 percent, the quality is easy to control, and the method is simple and rapid. In the invention, the research in the aspect of pharmacology is carried out on the icariside I and proves that the icariside I has a protective action for pituitrin-induced rat myocardial ischemia and is concretely shown as the action of inhabiting T waves and the action of inhabiting J point displacement change. The icariside I has the protective action for guinea-pig isolated hearts and is concretely shown as the action of increasing the coronary blood flow of guinea pigs. The icariside I obtained through the invention is used in preparing a medicament for treating heart diseases.

Description

technical field [0001] The invention relates to an extraction method and application of icariside I in Epimedium korean leaves. Background technique [0002] Epimedium is Berberidaceae plants Epimedium (Epimedium brevi2cornum Maximl), Epimedium sagitta tum (Sieblet Zuecl) Maximl], Epimedium pubescens (E1 pubescensMax2iml), Epimedium wushanense ( E1 wushanense T1 S1 Ying) or the dried above-ground part of Korean Epimedium (E1koreanam Nakai), has the effects of invigorating the kidney and strengthening yang, strengthening tendons and bones, expelling wind and dampness, and is often used to treat impotence, profuse urination, weak waist and knees, and crown Heart disease, chronic bronchitis, neurasthenia and polio. [0003] Modern chemical composition research shows that the main active ingredients of Epimedium are total flavonoids of Epimedium (icariin, icariin) and epimedium polysaccharides, and the active ingredients in the total flavonoids are considered to be 8-position ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H17/07C07H1/08A61K31/7048A61P9/00A61P9/10
Inventor 刘忠英刘志强丁一迪
Owner JILIN UNIV
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