Organ preservation solution and preparation method thereof

An organ preservation solution and phosphate technology, applied in the fields of medicine and biology, can solve the problems of poor organ preservation effect, limitation of multiple organ preservation solutions, inconvenience in handling and testing, etc., to maintain cell osmotic pressure, reduce ion exchange and The effect of energy consumption and prevention of cell edema

Inactive Publication Date: 2017-01-25
YANGZIJIANG PHARMA GROUP SHANGHAI HAINI PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, this preservation solution only has a good effect on the kidney, and the preservation effect on other organs is not good.
[0010] In summary, HTK solution and Celsior solution are mainly used for the preservation of the heart, and the scope of use is limited; UW solution has many advantages as a standard multi-organ preservation solution, but its viscosity is high, potassium ions are too high, and it is expensive. It is inconvenient to test and is not suitable for the actual situation of our country; the domestic HCA solution and its improved formula are mainly used for kidney preservation, and the preservation effect on other organs is not good
In recent years, with the increasing demand for large organ transplants such as liver, heart and pancreas, and combined multi-organ transplantation in my country, the lack of efficient, cheap, wide-ranging and adaptable multi-organ preservation solutions has become a limitation of organ transplantation technology in China. important reason for development

Method used

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  • Organ preservation solution and preparation method thereof
  • Organ preservation solution and preparation method thereof
  • Organ preservation solution and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0053] Embodiment 1 A kind of organ preservation solution

[0054] Formula, each 1000mL contains:

[0055]

[0056]

[0057] Preparation:

[0058] (1) Weigh other ingredients except adenosine according to the dosage of the formula, add 600mL of water for injection and stir evenly, add 1g of medical activated carbon with an average particle size of 2μm, stir for 5 minutes, use 0.45μm Decarburization by membrane filtration to obtain solution A;

[0059] (2) Weigh adenosine according to the dosage of the formula, dissolve it with 50mL of water for injection to obtain an energy substrate solution, mix the energy substrate solution with solution A of step (1), add water for injection to 1000mL, and stir evenly Finally, use a 0.45 μm filter membrane to filter, and the filtrate is the organ preservation solution.

Embodiment 2

[0060] Embodiment 2 A kind of organ preservation solution

[0061] Formula, each 1000mL contains:

[0062]

[0063]

[0064] Preparation:

[0065] (1) Weigh other ingredients except adenosine, coenzyme Q10 and succinic acid according to the dosage of the formula, add water for injection with a total volume of 800mL and stir evenly, then add 5g of medical activated carbon with an average particle size of 3μm, and stir for 20 minutes Finally, use a 0.45 μm filter membrane to filter and decarburize to obtain solution A;

[0066] (2) Weigh adenosine, coenzyme Q10 and succinic acid according to the dosage of the formula, and dissolve them in 100mL of water for injection to obtain the energy substrate solution, mix the energy substrate solution with solution A in step (1), and add additional injection Make up to 1000mL with water, stir evenly, and filter with a 0.45μm filter membrane, and the filtrate is the organ preservation solution.

Embodiment 3

[0067] Embodiment 3 A kind of organ preservation solution

[0068] Formula, each 1000mL contains:

[0069]

[0070]

[0071] Preparation:

[0072] (1) Weigh other ingredients except adenosine, coenzyme Q10, tryptophan and succinic acid according to the dosage of the formula, add up to 700mL of water for injection and stir evenly, then add 3g of medical activated carbon with an average particle size of 4μm , after stirring for 15 minutes, use a 0.45 μm filter membrane to filter and decarburize to obtain solution A;

[0073] (2) Weigh adenosine, coenzyme Q10, tryptophan and succinic acid according to the dosage of the formula, and dissolve them in 200mL of water for injection to obtain the energy substrate solution, and mix the energy substrate solution with the solution A of step (1) , add water for injection to 1000mL, stir evenly, and filter with a 0.45μm filter membrane, and the filtrate is the organ preservation solution.

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Abstract

The invention relates to the fields of medicine and biology, in particular to an organ preservation solution and a preparation method thereof. Every 1000mL of the organ preservation solution is prepared from the following components by weight: 3.0-15.0g of sodium citrate, 10.0-30.0g of potassium citrate, 5.0-15.0g of mannitol, 0.5-1.2g of potassium chloride, 0.5-1.8g of magnesium sulfate heptahydrate, 0.5-25.0g of a pH buffer pair, 0.2-25.0g of energy substrate and the balance of water for injection. The organ preservation solution also contains one or more of laminarin, trehalose, laminine and betaine. The organ preservation solution is low in price and efficient, and is suitable for preserving multiple organs.

Description

technical field [0001] The invention relates to the fields of medicine and biology, in particular to an organ preservation solution and a preparation method thereof. Background technique [0002] Organ transplantation is hailed as a major life science development that changed human life in the 20th century, and is an effective method recognized around the world to treat a variety of terminal diseases. According to statistics, in my country alone, there are 1-1.5 million patients with organ failure each year, and about 300,000 patients in urgent need of organ transplantation. Therefore, how to effectively preserve isolated organs has become one of the important supporting technologies for organ transplantation. [0003] The fundamental purpose of organ preservation is to maintain the viability of isolated organs, minimize various damages to isolated organs caused by ischemia, make them more suitable for transportation, and restore their functions more quickly after surgery. ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01N1/02
CPCA01N1/021A01N1/0215A01N1/0221A01N1/0226
Inventor 吴斌郑璐朱华荣丛海建高苇房莲相任波苏晓飞
Owner YANGZIJIANG PHARMA GROUP SHANGHAI HAINI PHARMA
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