Organ preservation solution and preparation method thereof
An organ preservation solution and phosphate technology, applied in the fields of medicine and biology, can solve the problems of poor organ preservation effect, limitation of multiple organ preservation solutions, inconvenience in handling and testing, etc., to maintain cell osmotic pressure, reduce ion exchange and The effect of energy consumption and prevention of cell edema
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Embodiment 1
[0053] Embodiment 1 A kind of organ preservation solution
[0054] Formula, each 1000mL contains:
[0055]
[0056]
[0057] Preparation:
[0058] (1) Weigh other ingredients except adenosine according to the dosage of the formula, add 600mL of water for injection and stir evenly, add 1g of medical activated carbon with an average particle size of 2μm, stir for 5 minutes, use 0.45μm Decarburization by membrane filtration to obtain solution A;
[0059] (2) Weigh adenosine according to the dosage of the formula, dissolve it with 50mL of water for injection to obtain an energy substrate solution, mix the energy substrate solution with solution A of step (1), add water for injection to 1000mL, and stir evenly Finally, use a 0.45 μm filter membrane to filter, and the filtrate is the organ preservation solution.
Embodiment 2
[0060] Embodiment 2 A kind of organ preservation solution
[0061] Formula, each 1000mL contains:
[0062]
[0063]
[0064] Preparation:
[0065] (1) Weigh other ingredients except adenosine, coenzyme Q10 and succinic acid according to the dosage of the formula, add water for injection with a total volume of 800mL and stir evenly, then add 5g of medical activated carbon with an average particle size of 3μm, and stir for 20 minutes Finally, use a 0.45 μm filter membrane to filter and decarburize to obtain solution A;
[0066] (2) Weigh adenosine, coenzyme Q10 and succinic acid according to the dosage of the formula, and dissolve them in 100mL of water for injection to obtain the energy substrate solution, mix the energy substrate solution with solution A in step (1), and add additional injection Make up to 1000mL with water, stir evenly, and filter with a 0.45μm filter membrane, and the filtrate is the organ preservation solution.
Embodiment 3
[0067] Embodiment 3 A kind of organ preservation solution
[0068] Formula, each 1000mL contains:
[0069]
[0070]
[0071] Preparation:
[0072] (1) Weigh other ingredients except adenosine, coenzyme Q10, tryptophan and succinic acid according to the dosage of the formula, add up to 700mL of water for injection and stir evenly, then add 3g of medical activated carbon with an average particle size of 4μm , after stirring for 15 minutes, use a 0.45 μm filter membrane to filter and decarburize to obtain solution A;
[0073] (2) Weigh adenosine, coenzyme Q10, tryptophan and succinic acid according to the dosage of the formula, and dissolve them in 200mL of water for injection to obtain the energy substrate solution, and mix the energy substrate solution with the solution A of step (1) , add water for injection to 1000mL, stir evenly, and filter with a 0.45μm filter membrane, and the filtrate is the organ preservation solution.
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