The invention discloses a primary hepatocyte cryopreservation solution, a hepatocyte cryopreservation method and a hepatocyte recovery method, and belongs to the field of primary hepatocyte cryopreservation and recovery. The primary hepatocyte cryopreservation solution comprises, by volume, 45% of a solution A, 10-45% of fetal bovine serum, 10% of dimethyl sulfoxide, and 0-35% of water, and the solution A comprises D-glucose, hydroxyethyl starch, lactobionic acid, polyvinylpyrrolidone, D-raffinose pentahydrate, potassium hydroxide, potassium dihydrogen phosphate, magnesium sulfate heptahydrate, adenosine, reduced glutathione, allopurinol, tauroursodeoxycholic acid, and diammonium glycyrrhizinate. The invention also discloses the hepatocyte cryopreservation method and the hepatocyte recovery method. The primary hepatocyte cryopreservation solution can reduce the damages of the primary hepatocytes in the cryopreservation process and improve the vitality and adherence efficiency of the primary hepatocytes after thawing, and can be widely used in basic research and new drug development for livers.