Mesenchymal stem cell preparation and preparing method and application thereof

A technology of mesenchymal stem cells and preparations, applied in the field of mesenchymal stem cell preparations and preparations for the treatment of rheumatoid arthritis, which can solve uncertain cell functions, inability to fully exert the biological properties of mesenchymal stem cells, and unfavorable arthritis recovery, etc. problems, to achieve the effect of improving the inflammatory microenvironment, enhancing the immune regulation ability and functional stability, and improving the immune regulation ability and function

Inactive Publication Date: 2019-10-25
山东佰鸿干细胞生物技术有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] The purpose of the present invention is to overcome the problems that the existing preparations for the treatment of arthritis have uncertain cell functions and cannot fully exert the biological properties of mesenchymal stem cells, which are not conducive to the recovery of arthritis.

Method used

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  • Mesenchymal stem cell preparation and preparing method and application thereof
  • Mesenchymal stem cell preparation and preparing method and application thereof
  • Mesenchymal stem cell preparation and preparing method and application thereof

Examples

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Embodiment 1

[0042] This embodiment provides a mesenchymal stem cell preparation, comprising the following components: mesenchymal stem cells, human serum albumin, low molecular weight heparin, compound amino acids, vitamin C and compound electrolytes; the mesenchymal stem cells are Cytokine-treated mesenchymal stem cells.

[0043]In this embodiment, the process of treating the mesenchymal stem cells with the inflammatory cytokines is as follows: take the mesenchymal stem cells that have been subcultured to the P4 generation, and inoculate them in α-MEM culture medium containing 10% FBS and inflammatory cytokines , at 37°C, with a concentration of 5% CO 2 After culturing in an incubator for 24-48 hours, the mesenchymal stem cells treated with inflammatory cytokines are obtained.

[0044] In this embodiment, the inflammatory cytokines are selected from TNF-α, and the final concentration of TNF-α in the culture medium is 20 ng / ml.

[0045] In some embodiments, the final concentration of TN...

Embodiment 2

[0053] This embodiment provides a method for preparing a mesenchymal stem cell preparation, comprising the following steps:

[0054] (1) Isolation and culture of mesenchymal stem cells: After isolating mesenchymal stem cells, they were inoculated in α-MEM medium containing 10% FBS, at 37°C, with a concentration of 5% CO 2 Cultivate in an incubator, and when the cell confluency is 80%-85%, digest and passage with 0.20% trypsin;

[0055] (2) Use inflammatory cytokines to induce mesenchymal stem cells: Take the above-mentioned mesenchymal stem cells that have been subcultured to the P4 passage, inoculate them in α-MEM medium containing 10% FBS and inflammatory cytokines, at 37°C, Concentration of 5 % CO 2 Cultivate in the incubator for 24-48h.

[0056] (3) Preparation of mesenchymal stem cell preparation: dissolve human serum albumin stock solution, low molecular weight heparin, compound amino acids, and vitamin C in compound electrolyte, pre-cool at 4°C for standby, digest and...

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Abstract

The invention provides a mesenchymal stem cell preparation. The preparation comprises mesenchymal stem cells, human serum albumin, low molecular heparin, compound amino acids, vitamin C and a compoundelectrolyte. The mesenchymal stem cells are processed by inflammatory cell factors. The mesenchymal stem cell preparation is suitable for relieving inflammation and repairing injured tissue in rheumatoid arthritis so as to realize the symptom relieving and function restoring of a patient.

Description

technical field [0001] The invention belongs to the field of biomedicine, and in particular relates to a mesenchymal stem cell preparation for treating rheumatoid arthritis and a preparation method thereof. Background technique [0002] Rheumatoid arthritis (RA) is a chronic, inflammatory synovitis-based systemic disease of unknown etiology. It is characterized by polyarticular, symmetrical, and aggressive arthritis of the small joints of the hands and feet, often accompanied by involvement of extra-articular organs and positive serum rheumatoid factor, which can lead to joint deformities and loss of function. RA is more common in women, and the incidence rate is 2 to 3 times that of men. It can occur at any age, and the high incidence age is 40 to 60 years old. The pathogenesis of RA may be related to heredity, infection, and sex hormones. The pathology of RA arthritis mainly includes the proliferation of synovial lining cells, the infiltration of a large number of infla...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/28A61K35/51A61K9/08A61P19/02A61P29/00C12N5/0775
CPCA61K35/28A61K35/51A61K9/0019A61K9/08A61P19/02A61P29/00C12N5/0665C12N5/0663C12N5/0667C12N5/0668C12N2509/00C12N2501/24
Inventor 高迎凤
Owner 山东佰鸿干细胞生物技术有限公司
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