Improved formulation of blood agar culture medium

A medium and blood agar technology, applied in the field of bioengineering, can solve the problems of poor repairing ability of damaged bacteria, not very comprehensive and balanced nutrient agar, and not very suitable, so as to repair damaged bacteria, facilitate cultivation and identification, The effect of maintaining osmotic pressure

Inactive Publication Date: 2017-03-08
哈尔滨亿隆科技有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the implementation of the new version of the Chinese Pharmacopoeia in 2015, TSA has completely replaced nutrient agar. The nutrient agar is not very comprehensive and balanced in terms of nutritional components, and its ability to repair damaged bacteria is poor, so it is no longer suitable for increasingly stringent testing.

Method used

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  • Improved formulation of blood agar culture medium

Examples

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Effect test

Embodiment 1

[0016] The improved formula of the blood agar medium provided in this example is composed of tryptone, beef extract powder, liver digestate, yeast extract, starch, sodium chloride, defibrated sheep blood and agar, wherein: in every 1000ml medium, Contains tryptone 15g, beef extract powder 5g, liver digestate 2.5g, starch 1g, yeast powder 5g, sodium chloride 5g, defibrated sheep blood 50ml, agar 15g, pH=7.2±0.2.

[0017] The preparation steps of the above-mentioned medium are as follows: mix tryptone, beef extract powder, liver digestate, starch, yeast extract powder, sodium chloride, and agar in proportion, add purified water to a constant volume and heat to dissolve, and adjust the pH value to 7.2±0.2 , sterilized at 121°C for 15 minutes, cooled to 50°C, added sterile defibrinated sheep blood in proportion, mixed well, poured into the plate, condensed to obtain the finished medium, and set aside.

[0018] The new blood agar basal medium prepared by using the above components ...

Embodiment 2

[0025] The difference between this example and Example 1 is that every 1000ml of medium contains 12g of tryptone, 4g of beef extract powder, 3g of liver digestate, 1.5g of starch, 6g of yeast powder, 6g of sodium chloride, and 45ml of defibrated sheep blood , Agar 12g, pH=7.2±0.2.

Embodiment 3

[0027] The difference between this example and Example 1 is that each 1000ml of culture medium contains 18g of tryptone, 6g of beef extract powder, 1.5g of liver digestate, 0.5g of starch, 4g of yeast powder, 4g of sodium chloride, and defibrated sheep blood. 55ml, 18g agar, pH=7.2±0.2.

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Abstract

The invention discloses an improved formulation of blood agar culture medium, made from tryptone, beef extract powder, liver digest, yeast extract, starch, sodium chloride, defibered sheep blood and agar, wherein each 1000 ml of the culture medium contains 10-20 g of tryptone, 3-8 g of beef extract powder, 1-4 g of liver digest, 0.5-2 g of starch, 3-8 g of yeast powder, 3-8 parts of sodium chloride, 40-60 ml of defibered sheep blood, 10-20 g of agar, and has pH of 7.2+/-0.2. The improved formulation can provide complete nutrition, maintain osmotic pressure for bacteria, and repair the damaged bacteria. The formulation optimized herein may meet the complete demands of common bacteria and most fastidious bacteria, and is good for the cultivation and identification of bacteria.

Description

technical field [0001] The invention belongs to the technical field of bioengineering, and relates to an improved formula of a blood agar basal medium, in particular to an improved formula of a blood agar basal medium that can meet the overall needs of common bacteria and most fastidious bacteria. Background technique [0002] The existing blood agar basal medium is basically made by adding sheep blood on the basis of nutrient agar (consisting of peptone, sodium chloride, beef extract powder and agar). Due to the implementation of the new version of the 2015 Chinese Pharmacopoeia, TSA has completely replaced nutrient agar. In terms of nutritional components, nutrient agar is not very comprehensive and balanced, and its ability to repair damaged bacteria is poor, so it is not suitable for more stringent testing today. Similarly, the blood agar basal medium established on the basis of nutrient agar also needs to be improved. Contents of the invention [0003] The purpose of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12R1/46C12R1/36
CPCC12N1/20
Inventor 李娜
Owner 哈尔滨亿隆科技有限公司
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