Exosome preserving fluid and exosome preserving method
A preservation method and technology of exosomes, applied in the biological field, can solve the problems of inability to freeze and thaw, affect the in-depth study of exosomes, and require high storage conditions, so as to lower the freezing point, inhibit biochemical reactions and the activities of most enzymes, protect The effect of stability
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Embodiment 1
[0053] This embodiment is an exosome preservation solution of the present invention, which includes 1 g / L bovine serum albumin, 30 g / L trehalose, 50% (v / v) glycerol, 5% (v / v) dimethyl sulfoxide and 0.01mol / L phosphate buffer; the pH value of the exosome preservation solution described in this example is 7.2.
Embodiment 2
[0055] This embodiment is an exosome preservation solution of the present invention, which includes 10g / L bovine serum albumin, 10g / L trehalose, 30% (v / v) glycerol, 10% (v / v) dimethyl sulfoxide and 0.02mol / L phosphate buffer; the pH value of the exosome preservation solution described in this example is 7.0.
Embodiment 3
[0057] This embodiment is an exosome preservation solution of the present invention, which includes 5g / L bovine serum albumin, 40g / L trehalose, 40% (v / v) glycerol, 8% (v / v) dimethyl sulfoxide and 0.015mol / L phosphate buffer; the pH value of the exosome preservation solution described in this example is 7.5.
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