33results about How to "Inactivation of protected invariance" patented technology

The invention relates to an exosome preserving fluid and an exosome preserving method, which belong to the technical field of biology. The exosome preserving fluid comprises bovine serum albumin, trehalose, glycerol, dimethyl sulfoxide and a phosphate buffer solution. The invention also provides the exosome preserving method comprises the following steps: heating the preserving fluid at 85-95 DEGC for 10-30 minutes to form a colloidal solution, and preserving the exosome in a colloidal solution. The exosome preserving fluid provided by the invention can maintain the integrity, biological activity and inclusion stability of exosomes for a long time.

The invention discloses a culture medium for rapid proliferation of neural stem cells. The culture medium is prepared from an RPMI-1640 culture medium, trehalose, recombinant human insulin, insulin growth factors, endothelial cell growth factors, potassium chloride, amino acid chelated selenium, glutamine, progesterone, coenzyme I, lipoic acid, vitamin, polygahatous polysaccharides, polysaccharide from radix codonopsis and ginseng saponin. The culture medium for rapid proliferation of the neural stem cells is good in cell wall adherence, fast in proliferation and free of animal source ingredients and does not produce residues or pollution, meanwhile the anti-pathogen microbial effect of the culture medium can be improved, and the cost of the culture medium can be reduced.

The invention discloses a crucian carp feed capable of preventing and treating a branchia hyperemia disease and a preparation method of the crucian carp feed, and belongs to the technical field of preparation of the crucian carp feed. The crucian carp feed is prepared from the following main raw materials of trash fish powder, corn protein powder, soybean flour, rice bran, yellow rapeseed meal, wheat bran powder, bentonite, radix isatidis, croton fruits, heartleaf houttuynia herbs, garlic, pumpkin seeds, chinaberry bark, common andrographis herbs, common cnidium fruits, radix sophorae flavescentis, Chinese galls, macleaya cordata, akebia stems, cyrtomium rhizomes, dioscorea zingiberensis, trehalose, propolis and calcium dihydrogen phosphate. According to the crucian carp feed disclosed by the invention, the bentonite is added, so that the production efficiency of a granulator can be effectively improved; Chinese herbal medicine substances having effects of resisting bacteria and killing insects and a natural antibacterial agent are added, so that pathogenic bacteria and pests of the branchia hyperemia disease can be effectively prevented and treated; effective components in Chinese herbal medicine substances are extracted by an ethanol backwashing method, so that the extraction rate is high. The crucian carp feed disclosed by the invention is rich in nutrition, can be used for effectively preventing and treating the branchia hyperemia disease of crucian carps and reducing the breeding risk of the crucian carps, and is worth popularization and application.

The invention discloses a placental tissue preservation liquid and a preparation method thereof. The placental tissue preservation liquid includes glucose injection, a serum replacement, gentamicin, amphotericin, trehalose and heparin lithium. The preparation method of the placental tissue preservation liquid includes adding the serum replacement, gentamicin, amphotericin B, magnesium ascorbyl phosphate hydrate, trehalose and heparin lithium into the glucose injection, wherein final concentrations of the ingredients are 0.5-2%, 800 U / mL, 15-30 ug / mL, 0.25-0.5 mg / mL, 0.6-3 mg / mL, and 20-40 U / mL. The placental tissue preservation liquid has clear composition and balanced nutrition, has no evident toxic and side effects on cells, can well maintain activity of in-vitro placental cells, and iseffective in preventing contamination of exogenous microorganisms introduced in the acquisition and transport steps.

The invention discloses a facial mask containing naringin and a preparation process thereof. Naringin is added to the mask liquid, the main component of naringin is flavonoids, and it works synergistically with niacinamide (B3) and pyridoxine hydrochloride (B6), so that the mask liquid has outstanding whitening effect; and When achieving the same whitening effect, the dosage of nicotinamide is effectively reduced, and the risk of skin allergy caused by the product is reduced; supplemented with β-glucan that can activate macrophages and neutrophils, the mask It can deeply clear free radicals and has the effect of radiation protection; at the same time, trehalose and polyglycerin are added to the mask liquid, and the two work together to effectively prolong the moisturizing time; finally, mild glycine, glutamic acid and Proline adjusts the pH value of the mask liquid to 4.0~5.3, which is close to the pH value of the body fluid, so that the mask liquid can penetrate deeply into the skin cells and achieve the best whitening and brightening effect.

The invention belongs to the field of animal food, and provides a functional food for a show dog and a preparation method thereof. The functional food includes a lucid ganoderma extract, a ginseng extract and an astragalus extract and further comprises the raw material components of vitamin A, vitamin B2, vitamin C and vitamin E as well as taurine, folic acid, trehalose, carotene, glucose, non-GMO soybean protein, yolk powder and milk powder; the preparation method mainly comprises the steps of mixing, sterilizing and packing, so as to obtain the food, as a result, the nutrition of the show dog can be quickly supplemented, the energy is increased, the physical fitness and attention are improved, and the fatigue recovery is quickened; the food raw materials are well-chosen natural raw materials, and meanwhile, no additives and preservatives are adopted.

The invention relates to low-salt and low-sugar green plum preserves and a preparation method thereof. The low-salt and low-sugar green plum preserves comprises the following ingredients in parts by weight: 1000 parts of salted plums, 35-45 parts of salt, 160-190 parts of high fructose maize syrup, 18-25 parts of mycose, 1-2 parts of honey, 1-2 parts of stevioside, 0.2-0.5 part of sucralose, 0.2-0.5 part of potassium sorbate, 0.1-0.5 part of cinnamic acid, 2-3 parts of alcohol and 2-4 parts of glacial acetic acid. The preparation method comprises the technological steps of washing the salted plums as the raw material, desalting, seasoning, draining, filling and packaging. According to the preparation method, the technological production processes of the green plum preserves are optimized; the production processes are shortened, and a large amount of labor consumed in a solarization working procedure is reduced greatly. The low-salt and low-sugar green plums preserve can reserve nutritive values and health functions of green plums very well, have the characteristics of low salt and low sugar, and meet pursuits of consumers on healthy and green products very well.

The invention discloses a humic acid water flush fertilizer applicable for secondary salinization land in Yellow River irrigated areas. The fertilizer is prepared from, by weight, 150-200 parts of bagasse, 0.5-2 parts of alginate oligosaccharide, 30-40 parts of livestock manure, 16-25 parts of microbial agent, 20-40 parts of humic acid, 10-20 parts of boracic acid, 10-15 parts of diatomite, 10-15 parts of magnesium sulfate and 10-20 parts of naphthylacetic acid. According to the humic acid water flush fertilizer applicable for secondary salinization of lands in Yellow River irrigated areas, the raw materials are reasonable in matching. Sedimentation and antagonism among the raw materials are overcome, soil physical and chemical properties are improved, hardening is removed, soil is loosened, the pH value of the soil is adjusted and a soil structure is improved. By adding alginate oligosaccharide in the water flush fertilizer, the humic acid water flush fertilizer applicable for secondary salinization land in Yellow River irrigated areas continuously provides nutrients for plants, has long action time, boosts each stage indispensable to growth cycles of the plants without any influence on the environment, and can further form effective protecting films on the surfaces of plant bodies to enhance disease resistance and tolerance resistance of the plant bodies.

The invention belongs to the technical field of skincare products and particularly relates to a skincare composition and a preparation method thereof. The skincare composition comprises, by weight, 2-20 parts of polyol moisturizing agent, 0.05-0.4 part of sodium hyaluronate, 0-0.2 part of xanthan gum, 0.5-2.5 parts of trehalose and 0-0.6 part of antioxidant. The skincare composition is sterile, free of preservatives, high in moisturizing and anti-oxidation effect, simple in preparation method and applicable to skincare products such as moisturizing spray, toner, essence and lotion.

The invention provides a functional food for racing dogs and a preparation method thereof, belonging to the field of animal food. The functional food of the present invention contains ganoderma lucidum extract, ginseng extract and astragalus extract, and the food raw materials also include vitamin A, vitamin B2, vitamin C, vitamin E, taurine, folic acid, trehalose, carotene, glucose, Non-GMO soy protein, egg yolk powder and milk powder. Its preparation method mainly includes the steps of mixing, sterilizing and packaging. The food thus prepared can quickly supplement the nutrition of racing dogs, improve energy, improve physical fitness, improve concentration, and speed up fatigue recovery; and the raw materials constituting the food are refined Selected natural raw materials, while no additives, no preservatives.

The invention provides a functional food used for calcium supplement for a pet and a preparation method thereof, and belongs to the field of animal foods. The functional food provided by the invention contains plant extracts namely a yam extract and a fragrant solomonseal rhizome extract, and further comprises raw materials namely the vitamin A, the vitamin B2, the vitamin C, the vitamin D, the vitamin E, as well as taurine, folic acid, mycose,calcium carbonate, carotene, glucosamine sulfate,non-genetically modified organism soybean protein, yolk powder and milk powder. The preparation method thereof mainly comprises the steps of mixing, sterilizing and packaging. With adoption of the food prepared through the preparation method, the growth of skeletons of the pet can be promoted, the loss of calcium can be reduced, and the bones and joints of the pet can be protected; moreover, the components of the raw materials of the food are all selected natural raw materials, and no addictive or preservative is used.

The invention discloses a cultivation method for increasing the virulence of porcine epidemic diarrhea virus, which comprises the following steps: dividing Vero cells into bottles for passage, then inserting the cell growth solution containing neonatal bovine serum, and obtaining a dense monolayer after cultivation; Inoculate porcine epidemic diarrhea virus in a dense monolayer, and cultivate the mixture; add maintenance solution containing newborn bovine serum, insulin and trehalose to the mixture, rotate the culture, harvest the virus when the lesion rate is greater than 80%, and obtain porcine epidemic diarrhea Virus. The invention can significantly increase the virus content, avoid virus inactivation, and effectively solve the problem of low virus price.

The invention provides a functional food used for hair beautification of a pet and a preparation method thereof, and belongs to the field of animal foods. The functional food provided by the invention contains plant extracts namely a fleece-flower root extract, a mulberry extract, a glossy privet fruit extract and a ginseng extract, and further comprises the vitamin A, the vitamin B2, the vitamin C, the vitamin D, the vitamin E, as well as taurine, folic acid, mycose, carotene, glucose,non-genetically modified organism soybean protein, yolk powder and milk powder. The preparation method thereof mainly comprises the steps of mixing, sterilizing and packaging. With adoption of the food prepared through the preparation method, the color of the hair of the pet can be improved, and the frequency of occurrence of allergic dermatitis can be reduced; moreover, the components of the raw materials of the food are all selected natural raw materials, and no addictive or preservative is used.

The invention relates to a method for prolonging storage time of edible fungus solid spawn. A solid culturing medium is prepared from, 20-25 g / L of trehalose, 2-6 g / L of yeast powder, 1-2 g / L of peptone, 1-2 g / L of monopotassium phosphate, 1.5-2 g / L of magnesium sulfate, 15-20 g / L of agar, 0.01-1.5 g / L of oxidized graphene, 0.1-0.85 g / L of N-butylpyridine hexafluorophosphate, 0.1-0.5 g / L of sodiumcarboxymethylcellulose, 0.2-0.8 g / L of polyacrylamide, 1.3-9.5 g / L of nano calcium carbonate, 0.2-0.8 g / L of laurinol sodium sulfonate, and the balance purified water. The pH value of the culturing medium is adjusted to 5-6. The method can prolong the storage time of the edible fungus solid spawn, hydroxyl groups and epoxy groups are randomly distributed on an oxidized graphene single sheet, in awater environment, partial hydroxyl groups and partial epoxy groups chemically react with hydroxymethyl cellulose, polyacrylamide and the nano calcium carbonate to form a compound even a polymer so as to reduce the activity of the culturing medium, and edible fungus can survive in a lower activity environment to prolong the storage time.