Culture method for increasing titer of porcine epidemic diarrhea viruses

Abstract

The invention discloses a culture method for increasing the titer of porcine epidemic diarrhea viruses. The culture method includes the following steps: Vero cells are subjected to bottle-separation passage, and then inoculated into cell growth fluid containing newborn calf serum to be cultured to obtain a dense single layer; then the porcine epidemic diarrhea viruses are inoculated into the densesingle layer to be cultured to obtain a mixture; and maintenance media containing the newborn calf serum, insulin and trehalose are added into the mixture, rotating culture is conducted, when the lesion rate is greater than 80%, the viruses are obtained, and thus the porcine epidemic diarrhea viruses are obtained. The content of the viruses can be significantly increased, virus inactivation is avoided, and the problem of low virus titer is effectively solved.

Description

technical field

[0001] The invention relates to the technical field of porcine virus cultivation, in particular to a cultivation method for increasing the virulence of porcine epidemic diarrhea virus. Background technique

[0002] Porcine Epidemic Diarrhea Virus (PEDV) belongs to class Ⅰ coronavirus, which causes porcine epidemic diarrhea disease. Piglets infected with this virus have a high mortality rate. The morphological characteristics of PEDV in intestinal epithelial cells are the same as those of other coronaviruses, and the virus is assembled by budding through the endoplasmic membrane. However, the virus particles detected in fecal samples are pleomorphic and tend to be spherical, with a diameter of about 95-190nm (including fibrils). Most virions have an electron-opaque central region with swollen fibrils 18-23 nm long, arranged radially outward from the nucleocapsid. When producing the virus liquid antigen of porcine epidemic diarrhea virus (PEDV), although it c...

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