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45results about How to "Sustain life" patented technology

Preparation method of serum-free freezing medium of human umbilical cord mesenchymal stem cells (hUC-MSCs)

The invention relates to the technical field of freezing mediums, and discloses a preparation method of a serum-free freezing medium of human umbilical cord mesenchymal stem cells (hUC-MSCs). The serum-free freezing medium of the hUC-MSCs is prepared from the following components in percentage by volume: 20-80% of a basal culture medium, 5-30% of platelet lysate, 1-10% of instant-dissolving protein polysaccharide mother liquor, and 5-25% of trehalose mother liquor. According to the preparation method of the serum-free freezing medium of the hUC-MSCs, the platelet lysate and trehalose are usedfor replacing original animal serum and DMSO, and thus the preparation method has the advantages that the activity of the UC-MSCs can be maintained for a long time, the survival rate of the cells is significantly increased, and preparation is easy and safe; and the problems that the DMSO serves as a permeating protective agent and can penetrate into the cells quickly, the time that the cells are exposed in the harmful environment is shortened, however, the DMSO has the toxic effect on the cells at the room temperature, operation at the room temperature needs to be quick as soon as possible, FBS can increase the probability of animal pathogenic pollution, and consequently, uncertain factors are added for future using of the cells are solved.
Owner:青岛麦迪赛斯生物科技有限公司

Carp electrode transforation implantation brain stereotaxic method

The invention discloses a carp electrode transforation implantation brain stereo positioning method, which belongs to the technical field of brain stereo positioning. The invention mainly solves the problem of accurate positioning in electrode implantation into brain without craniotomy. The method comprises the following steps: drawing a straight line centrally from the carp mouth to the intersection point between the head and the first scale of the trunk, and drawing a straight line parallel to the central line based on the upper edges of two orbits respectively; drawing a connection line along the front edges of the two orbits; drawing a connection line along the rear edges of the two orbits; drawing a connection line between the joint moving joint points of the operculum bones on the two sides and the orbit bones; and drawing a line through the joint between the head and the first scale of the trunk and vertical to the central line. The skull surface is divided into 6 areas by the 7 lines, each area representing a coordinate system. The mouth, dorsal fin and operculum on the two sides clamp 4 points for fixing; in the positioning of a brain stereotaxic instrument, an electrode is implanted into the brain along a skull hole, and magnetic resonance imaging equipment navigates and corrects deviation so as to realize three-dimensional accurate positioning without craniotomy. The invention is applicable to the collection of brain wave of aquatic animals or control on biological behavior by conduction of electrical signals.
Owner:YANSHAN UNIV

Crucian carp feed capable of preventing and treating branchia hyperemia disease and preparation method of crucian carp feed

The invention discloses a crucian carp feed capable of preventing and treating a branchia hyperemia disease and a preparation method of the crucian carp feed, and belongs to the technical field of preparation of the crucian carp feed. The crucian carp feed is prepared from the following main raw materials of trash fish powder, corn protein powder, soybean flour, rice bran, yellow rapeseed meal, wheat bran powder, bentonite, radix isatidis, croton fruits, heartleaf houttuynia herbs, garlic, pumpkin seeds, chinaberry bark, common andrographis herbs, common cnidium fruits, radix sophorae flavescentis, Chinese galls, macleaya cordata, akebia stems, cyrtomium rhizomes, dioscorea zingiberensis, trehalose, propolis and calcium dihydrogen phosphate. According to the crucian carp feed disclosed by the invention, the bentonite is added, so that the production efficiency of a granulator can be effectively improved; Chinese herbal medicine substances having effects of resisting bacteria and killing insects and a natural antibacterial agent are added, so that pathogenic bacteria and pests of the branchia hyperemia disease can be effectively prevented and treated; effective components in Chinese herbal medicine substances are extracted by an ethanol backwashing method, so that the extraction rate is high. The crucian carp feed disclosed by the invention is rich in nutrition, can be used for effectively preventing and treating the branchia hyperemia disease of crucian carps and reducing the breeding risk of the crucian carps, and is worth popularization and application.
Owner:GUANGXI ZHIBAO TECH CO LTD

Whitening and freckle-removing cosmetic containing negundo chastetree fruit stem cells and preparation method of whitening and freckle-removing cosmetic

The invention discloses a whitening and freckle-removing cosmetic containing negundo chastetree fruit stem cells. The whitening and freckle-removing cosmetic mainly consists of the following components in percentages by weight: 5% to 30% of negundo chastetree fruit stem cells, 2% to 15% of a ginseng extracting solution, 2% to 15% of a prepared rehmannia root extracting solution and 2% to 15% of a lemon extracting solution. The negundo chastetree fruit stem cells are selected and used as a main raw material, the cells are activated, free radicals are scavenged, the generation of chloasma and butterfly rash is blocked, skin melanin is eliminated, whelk is reduced and removed, the skin is white and beautified, the negundo chastetree fruit stem cells are of high compatibility with human bodies and are more easily absorbed by the human bodies, the cosmetic has a good promotion effect for the growth of skin cells, the metabolism of the cells can be quickened, the activity of skin cells is strengthened, and moreover, the prepared radix rehmanniae extract, the ginseng extract and the lemon extract with the effects of whitening and activating skin, promoting metabolism and strengthening skin cell activity are added, and generate a synergistic effect with the negundo chastetree fruit stem cells, so that the skin type can be obviously improved, the skin generation capacity is improved, and the whitening and freckle-removing effects are obvious.
Owner:GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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