Skin external composition and application thereof

An external composition and skin technology, applied in the field of skin repair, can solve problems such as low extraction rate, inconvenient liquefaction, lack of skin care products, etc., and achieve the effects of high skin, stable product properties, and increased skin elasticity and plumpness

Inactive Publication Date: 2019-10-18
浙江梵泊细胞工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] However, due to the low extraction rate of exosomes from mesenchymal stem cells and the inconvenient preservation of liquefied state, the...

Method used

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  • Skin external composition and application thereof
  • Skin external composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0044] Example 1 Preparation of freeze-dried powder of human umbilical cord mesenchymal stem cell exosomes

[0045] 1.1 Collection of human umbilical cord mesenchymal stem cells

[0046] a. Collect healthy human umbilical cord, further clean the umbilical cord, and then cut the umbilical cord into small tissues with sterile surgical scissors; b. Digest the umbilical cord tissue block by adding appropriate concentrations of pancreatin, collagenase, etc. For 3 hours, monocytes were differentiated, and the differentiated monocytes were collected and cultured in a serum-free medium to obtain umbilical cord mesenchymal stem cells.

[0047] 1.2 Perform cell culture

[0048] Place the collected mesenchymal stem cell single cell suspension at 37°C with a volume fraction of 6.5% CO 2 Cultivate in an incubator and pass through the cells after 90% fusion. Collect 4th generation cells as cultured cells; b. Select cells in the logarithmic growth phase among the cultured cells, add serum-free mediu...

Embodiment 2

[0054] A lotion containing mesenchymal stem cell exosomes freeze-dried powder, characterized in that it contains the following components by weight percentage: mesenchymal stem cell exosomes freeze-dried powder (prepared in Example 1) 5.0%, Glycerin 1%, polyol A 3%, betaine 0.5%, panthenol 0.8%, dipotassium glycyrrhizinate 0.1%, other active ingredient A 1.0%, polyethylene glycol-3 1.5%, surfactant A 0.3% , Preservative A 0.2%, xanthan gum 0.15%, fragrance 0.03%, the balance is purified water; wherein, the polyol A is butanediol; the other active ingredients A are allantoin and nicotinamide The mass of the three is 1:1:1; the surfactant A is a mixture of hydroxyethyl cellulose and PEG-40 hydrogenated castor oil, and the mass ratio of the two is 1:1; The preservative A is methyl paraben. Prepare the lotion according to conventional methods in the art.

Embodiment 3

[0056] A facial mask essence containing mesenchymal stem cell exosomes freeze-dried powder, which is characterized in that it contains the following components in weight percentage: mesenchymal stem cell exosomes freeze-dried powder (prepared in Example 1) 6.0% , Glycerin 1%, Polyol B3%, Panthenol 0.8%, Dipotassium Glycyrrhizinate 0.05%, Allantoin 0.3%, Other Active Ingredient B 1.0%, Surfactant B3.0%, Preservative B 0.3%, Xanthan gum is 0.2%, fragrance is 0.05%, and aminomethylpropanol is added. The content of aminomethylpropanol is based on adjusting the pH value of the facial mask essence to 5.5-7, and the balance is purified water; The polyol B is 1,3-propanediol; the other active ingredient B is a mixture of nicotinamide and sodium hyaluronate with a mass ratio of 1:1; the surfactant B is hydroxyethyl fiber The mass ratio of the mixture of vegetable, isopropyl isostearate and polyglycerol-2 oleate is 1:1:1; the preservative B is propylparaben. The facial mask essence is p...

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Abstract

The present invention relates to the field of skin repair, and provides a skin external composition. The skin external composition comprises mesenchymal stem cell exosome lyophilized powder, wherein the lyophilized powder contains at least 25.0-35.0 ng/g of fibronectin, 5.0-14.5 ng/g of a vascular endothelial growth factor, 5.5-15.5 ng/g of a fibroblast growth factor, 5.0-10.0 ng/g of an epithelial cell growth factor, 25.0-30.0 ng/g of a hepatocyte growth factor, 7.5-17.5 ng/g of a transforming growth factor, 0.5-5 ng/g of 1-sphingosine phosphate, and 0.5-5 ng/g of ceramide-1-phosphoric acid.The skin external composition provided by the invention comprises the mesenchymal stem cell exosome lyophilized powder having a repairing effect on the skin, does not contain medium components or cellular components, avoids damage to the human body and the skin by adding a cytotoxic substance and avoids the potential hazard of skin allergies. The product is more stable after being prepared as lyophilized powder so as to exert biological activity for a longer period of time.

Description

Technical field [0001] The invention belongs to the technical field of skin repair, and specifically relates to a composition for external use on skin and its use. Background technique [0002] The skin is the largest organ of the human body and covers the surface of the human body. It has the functions of protection, sensation, and regulation of body temperature. Its structure is from top to bottom, followed by epidermis, dermis and hypodermis. As people grow older, the signs of skin aging will gradually appear. The causes of skin aging can be divided into internal and external causes. [0003] Intrinsic aging refers to the body’s natural aging process, including the gradual decrease of keratinous stem cells as we age, resulting in basal cell disorders, reduced hyaluronic acid, and loss of moisturizing factors; decreased secretion of hormones (such as sebaceous glands) slows down the overall The metabolism of the skin ultimately makes the skin lack of tension and elasticity; the ...

Claims

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Application Information

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IPC IPC(8): A61K8/99A61K8/64A61K8/55A61Q19/08A61Q19/00
CPCA61K8/99A61K8/64A61K8/55A61Q19/08A61Q19/00
Inventor 王峰陈悦
Owner 浙江梵泊细胞工程有限公司
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