68results about How to "Avoid the possibility of contamination" patented technology

The invention provides a real-time fluorescence quantification PCR method with magnetic bead nucleic acid extraction and amplification conducted in one tube. A lysis solution mixed with magnetic beads and a sample to be detected are added to a PCR amplification tube, even mixing and standing are conducted, mixed liquor is extracted after magnetic extraction, and obtained magnetic beads are washed once; a well prepared PCR liquid is added into the PCR amplification tube for real-time fluorescence quantification PCR of target nucleic acid; the lysis solution is prepared from 0.2-0.4N sodium hydroxide, 0.3-0.6M potassium chloride, 0.01-0.05%N-sodium lauroyl sarcosine, 5mM EDTA, 0.3-0.6M Tris-HCL, and 1-2% Triton X-100. According to the method, heating is not needed, indoor-temperature pyrolysis is conducted for about 5-10 min only, static washing is needed only once, and then laboratory pollution and magnetic bead nucleic acid loss are reduced, pollution possibility brought by step-by-step operation is avoided and detection time is shortened. By taking HBV DNA quantification as an example, sensitivity can be as low as 5 IU/ML, and repeatability is high.

The present invention concerns an antibody specific for human ALK (Anaplastic Lymphoma Kinase), in particular a scFv, a nucleic acid sequence encoding it, its production and its use as a pharmaceutical or for diagnostic purposes. Said antibody is suitable for the local treatment of tumors, in particular glioblastoma.

The invention discloses a rapid synthesis method of an indium phosphide polycrystalline material, which comprises the following steps of: (I) performing surface cleaning treatment on indium; (II) placing a multi-tubular quartz phosphorus bubble with phosphorus into a phosphorus source furnace; (III) placing the pre-designed thermal insulation system, heater, crucible with indium, phosphorus source furnace and seed crystal and B2O3 into a high-pressure single-crystal furnace hearth; (IV) vacuumizing the inside of the furnace and filling high-purity argon; (V) heating to vaporize phosphorus in the phosphorus bubble and injecting into indium melt, and reacting to generate indium phosphide; and (VI) growing crystal. The multi-tubular quartz phosphorus bubble comprises a quartz phosphorus container, at least two quartz phosphorus bubble tubes and a quartz cover. By adopting a multi-tubular quartz phosphorus bubble, the method disclosed by the invention realizes a rapid multi-tubular phosphorus injection synthesis technology, solves the problems of long synthesis time, non-uniform melt proportioning, impurity pollution and the like in the original technology, realizes rapid, efficient and high-purity synthesis of the indium phosphide material, and performs InP single crystal growth more easily.

The invention provides a fast multiplex PCR identification diagnosis detection method of invasive fungus infection based on cfDNA (cell free DNA). The method can be used for identifying the invasive infection caused by clinic common and high-incidence Candida albicans, Candida tropicalis, Candida parapsilosis, Candida krusei, Candida glabrata and Aspergillus fumigtus. An amplification primer is designed according to characteristic genome segments of each fungus category and species; a detection fluorescence probe of a strain can be distinguished according to the amplification segment design; the real time PCR can be performed on a sample to be tested; high sensitivity of nested PCR and high specificity and multi-target performance advantages of multiple fluorescent hybrid probe PCR are integrated for identifying the fungus strain. The invention also provides a PCR diagnosis kit for the invasive fungus infection and application thereof.

The invention discloses a lithium ion battery containing a high-thermal-conductivity and high-conductivity phase change composite material and a conductive agent. The high-thermal-conductivity and high-conductivity phase change composite material comprises a porous graphene substrate and an oxidized graphene phase change nanocapsule adsorbed in the substrate. The high-thermal-conductivity and high-conductivity phase change composite material provided by the invention has the characteristics of high thermal conductivity and high conductivity of graphene and the characteristic of high thermal conductivity of oxidized graphene, has the advantages of high heat conduction efficiency, high conductive performance and large energy storage capacity, is used in a high-specific-energy lithium ion battery, and can quickly absorb large amounts of heat generated in an abuse test; meanwhile, the oxidized graphene has a flame-retardant effect, so that the safety of the lithium ion battery in the abuse test is greatly enhanced; and moreover, due to the characteristic of high thermal conductivity of the graphene substrate and an oxidized graphene wall material, the heat generated by the high-specific-energy lithium ion battery during use can be effectively diffused, so that the temperature rise of the battery during use is effectively reduced and the service life of the battery is prolonged.

The invention discloses a method for cell surface functional coating modifying. The method comprises the following steps: putting cells into a dopamine solution prepared by a Tris-hydrochloric acid buffer solution; performing oscillating reaction for 20 minutes to 8 hours; enabling dopamine to generate oxidative polymerization on a cell membrane surface; enabling a dopamine quinone compound and dopamine to generate disproportionated cross-linking reaction; forming a composite layer on the cell surface; and modifying the cell surface functional coating for enabling the cell to keep cell activity in an unfriendly environment. The method is gentle in condition and is simple to operate; and moreover, the whole process is completed in an aqueous solution, so that possibility of generating pollution on environment by the solvent is avoided; and functional coating modification, on the whole cell surface, by dopamine is achieved through one step.

The invention discloses a method for simultaneously measuring five heavy metal elements including arsenic, cadmium, copper, mercury and lead in artificial tiger bone meal. The method includes main steps of 1), digesting artificial tiger bone meal samples by the aid of nitric acid and hydrogen peroxide and sizing the artificial tiger bone meal samples by the aid of 2% nitric acid solution to obtain analysis to-be-measured sample solution; 2), selectively preparing serial mixed standard solution from As, Cd, Cu, Hg and Pb single-element standard solution by the aid of 2% nitric acid solution; 3), plotting standard curves; 4), measuring the analysis to-be-measured sample solution by the aid of ICP-AES (inductively coupled plasma-atomic emission spectrometry) spectrographs so as to obtain the contents of the five heavy metal elements in the samples according to the standard curves. The method has the advantages that reaction for digesting the samples is mild, and accordingly the samples are low in pollution and loss; the digestion time can be shortened, digested samples can be directly measured by the aid of ICP-AES processes, and accordingly the detection cycles of the samples can be shortened; the method is high in safety and good in accuracy and repeatability, and requirements on quickly measuring the heavy metal elements in the artificial tiger bone meal can be met.

The invention discloses a membrane nucleic acid amplification sealing reaction tube capable of preloading a reagent. A liquid storage tube body is sleeved with a reaction tube body and sealed at the mouth portion of the reaction tube body, and the mouth portion of the liquid storage tube body is blocked by a seating cap body; a reaction pond capable of containing polymerase liquid is arranged in the reaction tube body, and a liquid storage pond capable of containing buffering liquid is arranged in the liquid storage tube body; the bottom of the liquid storage pond is isolated from the reactionpond through a plastic thin film to prevent the buffering liquid from mixing with the polymerase liquid; the plastic thin film is adopted to perform physical isolation, a user does not need to additionally prepare any other reagent, and operation procedures are greatly simplified; the sealing cover body is not needed to be opened during the whole operation process, and the possibility of aerosolcontamination is effectively avoided; in addition, the preloading of the reagent is achieved while the stability and period of effectiveness of the preloaded reagent are not changed, massive production of all spare parts can be achieved through injection molding, application cost is significantly reduced, and the reaction tube has more significant practical value.

The invention discloses a lysis solution for extracting nucleic acid by a paramagnetic particle method. The lysis solution contains: potassium hydroxide with a final concentration of 0.3-0.5, sodium acetate with a final concentration of 0.2-0.4, N-sodium lauroyl sarcosine with a mass fraction of 0.03-0.07%, EDTA with a final concentration of 3-7mM, Tween 20 with a volume fraction of 0.5-1.5%, andtrehalose with a final concentration of 0.1-0.3%. When used for experiments, the lysis solution or a kit provided by the invention can realizes the effects that: samples can be added without uniform mixing and heating; nucleic acid lysis requires no oscillation; magnetic beads do not need to be blown away during rinsing; only one static soaking is required to reduce laboratory contamination and nucleic acid loss; a reaction liquid and magnetic beads do not need to be uniformly mixed; the nucleic acid amplification does not need elution; and the obtained magnetic bead nucleic acid completely participates in the PCR amplification reaction, so that the possibility of pollution caused by tube transfer is avoided; the detection time is greatly shortened; and the repeatability of a low-value sample is good.

The invention discloses anti-pollution storage equipment for a clinical laboratory. The anti-pollution storage equipment at least comprises a storage rack and a shell, the storage rack is rotatably mounted in the shell, a plurality of sample storage boxes used for storing samples are mounted in the storage rack in a nested manner, and permanent magnets used for driving the sample storage boxes to slide in the radial direction of the shell are arranged at the first ends of the sample storage boxes; electromagnets are arranged at the positions, corresponding to the permanent magnets, of the storage rack. Under the condition that the electromagnets are electrified, the sample storage box is propped against or far away from the electromagnets in a manner of sliding in the radial direction of the shell based on magnetic force generated between the electromagnets and the permanent magnets. Samples can be effectively prevented from being polluted, the temperature of the sample storage environment can be maintained, the samples are convenient to take and place, and the working efficiency of a user can be greatly improved.

The invention discloses a processing device for an edible fungus deep-processed food. The device comprises a closed fungus container, a stirring mechanism and a rotating shaft, the fungus container isof a cylindrical structure, the stirring mechanism is arranged at the bottom of an inner cavity of the fungus container, the rotating shaft penetrates the top of the fungus container along the axialdirection, the rotating shaft is rotationally arranged, the rotating shaft is provided with a feeding cavity along the axial direction, the feeding cavity extends downwards along the axial direction of the rotating shaft and communicates with the inner cavity of the fungus container, the rotating shaft is provided with a feeding port, the feeding port communicates with the feeding cavity, the wallbody of the fungus container is provided with air holes, the air holes communicate with the inner cavity of the fungus container, and the air holes are externally connected to vacuumizing equipment.The processing device and processing method for the edible fungus deep-processed food can greatly reduce the hand labor intensity and simplify the operation steps of processing.

The invention is a single use delivery device capable of delivery of an agent (e.g., vaccine, drug, medicament, etc.) in a controlled manner and without requiring specialized skill in administering delivery of such agent. The delivery device is configured to be filled on-site and in the field with a dose of an agent, while remaining sterile and preventing the potential for contamination during thefilling process. The delivery device is configured to be fitted with various types of administering components, including, but not limited to, needles, nozzles for producing spray or droplets, and the like. The delivery device is further configured to be rendered incapable of reuse followings its intended use of delivering the fluid agent to a patient, thereby preventing reuse of the device and reducing the risk of the spreading blood-borne diseases through reuse.

The invention provides full-automatic freeze drying pharmaceutical equipment, which comprises a bottle washing machine, a tunnel type drying oven connected with the bottle washing machine, a filling machine connected with the tunnel type drying oven, an aligning machine connected with the filling machine, a conveying belt connected with the aligning machine, and a freeze dryer connected with the conveying belt. The equipment enters and exits automatically in a way of combining filling, freeze drying and capping processes, thereby eliminating manual operation, greatly increasing the production efficiency, and avoiding the possibility of pollution caused by manual operation.

A process for preparing the alpha-16 interferon includes optimizing the gene sequence of alpha-16 interferon, choosing proper expression system, reconstructing the engineering bacterial strain able to effectively express it, and creating the fermenting and purifying technology with galactose or lactose. Its advantages are high yield, high expression and low cost.

The invention provides high-energy-storage and high-efficiency bismuth ferrite-strontium titanate ceramic and a preparation method thereof. The preparation method comprises the following steps: adding MnO2 into 0.6 BiFeO3-0. 4SrTiO3, carrying out ball milling, drying, briquetting, sieving, carrying out cold isostatic pressing, and carrying out buried sintering treatment to obtain a 0.6 BiFeO3-0. 4SrTiO3-xwt% MnO2 ceramic sample. According to the 0.6 BiFeO3-0. 4SrTiO3-xwt% MnO2 lead-free antiferroelectric ceramic material provided by the invention, the volatilization of Bi is effectively inhibited, the problems that bismuth ferrite-based ceramic is easy to generate a second phase and is not compact in sintering are solved, relatively good energy storage performance can be ensured theoretically, the preparation process is simple, the repeatability is relatively good, the material is green and environment-friendly, and the preparation method is suitable for industrial production. The invention provides a novel lead-free energy storage material matrix.

The invention relates to a measurement method for total chlorine content in oil. Firstly, 2, 4, 6-trichlorophenol standard solution is prepared; then, a multiEA5000 type multi-element analyzer is used for detecting; standard curves of electric quantity integrals and total chlorine volume concentrations are drawn; and after an oil sample to be measured is detected, the regression calculation is implemented through the standard curves to obtain the total chlorine content in the oil to be measured. The measurement method for the total chlorine content in the oil has the advantages of simplicity, accuracy in measurement and high detection sensitivity, and provides a novel screening method for identifying dining room-kitchen waste oil and proper edible oil.

The invention discloses an environment-friendly type high-density wood and bamboo fiber board. The environment-friendly type high-density wood and bamboo fiber board is composed of 50%-60% of wood fibers, 20%-30% of bamboo fibers, 20%-30% of pvc, 8%-15% of medical stones and 1%-3% of environment-friendly fire retardant. A processing method for the environment-friendly type high-density wood and bamboo fiber board includes the steps of slicing, smashing, kneading, hot pressing and drying, and the environment-friendly type high-density wood and bamboo fiber board is manufactured, wherein density of the environment-friendly type high-density wood and bamboo fiber board is generally 880 kg/m<3> or above, and the thickness ranges from 2 mm to 30 mm. The environment-friendly type high-density wood and bamboo fiber board has the beneficial effects that releasing of free formaldehyde is avoided, the waterproof performance is good, the wear resisting performance is good, the flame retardant property is good, the texture of a plate face is smooth, the internal organizational structure is fine, and especially a tight edge is achieved; and the environment-friendly type high-density wood and bamboo fiber board is suitable for replacing traditional medium-and-high-density fiber boards and natural wood for serving as structural materials.

The invention discloses urea compound organic fertilizer for corns. The urea compound organic fertilizer is prepared from distillers' grains, plant ash, humic acid, urea, bentonite, potassium dihydrogen phosphate, calcium phosphate, charcoal powder, a waste edible mushroom culture medium, ammonium sulfate, potassium sulfate, a water-retaining agent, ammonium molybdate and sodium benzoate. The compound organic fertilizer for the corns, disclosed by the invention, has various different nutrient components and can meet requirements of the corns on fertilizer at different periods in a growth process; the fertility loss can be effectively reduced and the utilization rate of the fertilizer is improved. Therefore, the number of times of applying the fertilizer is reduced, the application amount is reduced and wastes are avoided; meanwhile, the possibility of causing pollution to a peripheral soil environment is avoided and underground water is not polluted.

The invention discloses a method, primers and a kit for detecting PD-L1 gene expression quantity through real-time fluorescent quantitative PCR. The method comprises the following steps: (1) synchronously extracting DNA and mRNA in a sample; (2) synthesizing a first chain cDNA by taking the to-be-detected mRNA as a template through a special reverse transcription primer; and (3) carrying out dual-channel real-time fluorescent quantitative PCR amplification by respectively adopting a primer corresponding to the specific sequence on the reverse transcription primer and a specific primer corresponding to a reference gene and taking a reverse transcription product cDNA and reference gene DNA as templates while amplifying a target gene cDNA and a reference gene, and calculating the copy numberratio of the target gene to the reference gene in the sample according to the respective standard curves by taking a plasmid constructed by linking the reference gene and the target gene sequence as astandard substance to obtain the expression level of the target gene, so that various experimental operation errors are synchronously corrected, and the reliability and the repeatability of the result are ensured.

The invention discloses a spiral ribbon-free vibrating pharmaceutical powder mixing device, relating to the field of pharmaceutical production equipment. The spiral ribbon-free vibrating pharmaceutical powder mixing device comprises a base, an elastic support frame, a vibration source mechanism, an elastic transition connection assembly and a mixing tank, wherein the elastic support frame is composed of a sliding guide column, a hinged block, a spring I, a lower bottom plate and an upper cover plate, the vibration source mechanism is composed of a motor and a cam, the motor is fixedly mountedon the base, the cam is fixedly connected with an output shaft of the motor, the elastic transition connection assembly is composed of a rotating wheel, a sliding connecting rod and an elastic hingedblock, the rotating wheel is fixedly connected with the sliding connecting rod and is in contact with the cam, the sliding connecting rod is hinged with the elastic hinged block and is connected withthe base in a sliding manner, the mixing tank is hinged with the hinged block and is fixedly connected with the elastic hinged block. The spiral ribbon-free vibrating pharmaceutical powder mixing device has a simple and reasonable structure; with a vibrating rolling type mixing way, the mixing speed and the mixing efficiency are both enhanced; by abandoning a conventional spiral ribbon stirring structure, pharmaceutical powder can be cleaner.

The invention discloses an efficient La-doped energy-storage linear dielectric ceramic material for a high temperature. A preparation method of the material comprises the following steps: firstly, taking SrCO3, CaCO3, TiO2 and La2O3 as raw materials, and proportioning and selecting the raw materials according to a chemical formula (Ca0.5Sr0.5)1-1.5xLaxTiO3; mixing the powder by wet ball-milling; pre-sintering the dried powder at a temperature of 1200 DEG C for 2 hours; performing secondary ball-milling, screening and molding; finally sintering at a temperature of 1400 DEG C for 2 hours to obtain the required linear dielectric ceramic material. The prepared ceramic material disclosed by the invention has the advantages of simple preparation process, low raw material price, and low production cost; a linear ferroelectric hysteresis loop can be obtained at room temperature, the energy storage density can be up to 2.07 J/cm<3>, which is 3 times that of non-doped SrTiO3, and the energy storage efficiency can be 94 percent or above at a high temperature of 200 DEG C.