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Method for treating ingredients of biological fluid

A technology of biological liquid and separation chamber, which is applied in the direction of biological testing, preparation of test samples, material inspection products, etc. It can solve the problems of increasing sample pollution, increasing operation steps, pollution, etc., so as to simplify separation and detection work and improve separation The effect of detection efficiency

Inactive Publication Date: 2010-01-06
上海奇康生物科技开发有限公司
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  • Abstract
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Problems solved by technology

If these indicators are to be evaluated, samples must be re-sampled from the separated samples for testing. These operations not only increase the number of steps, but also undoubtedly increase the chance of sample contamination.
For biological samples, the consequences of contamination are very serious, and in many cases even irreparable, leading to more serious consequences

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  • Method for treating ingredients of biological fluid
  • Method for treating ingredients of biological fluid

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Embodiment Construction

[0041] In the method for processing biological fluid components of the present invention, the separation and detection of the components in the biological fluid are completed in an integrated device. The method for processing biological fluid components of the present invention includes the following basic steps: transferring the biological fluid in the sample container containing the biological fluid to be separated to a centrifugal separation device; after settling in the centrifugal chamber device, the centrifugal device rotates around the axis to separate the biological fluid components; and the separated components are extruded while rotating, and the separated components are distributed in the container containing the separated samples through the flow path device; when the target component begins to appear, the branch circuit is opened to collect a small amount of sample for detection.

[0042] The basic composition of the device used by the inventive method is as attach...

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Abstract

The invention provides a method for treating ingredients of biological fluid, which comprises the following basic steps: the biological fluid in a sample vessel which holds the biological fluid to be separated is transferred to a centrifugal separation device; after the biological fluid is settled in a centrifugal chamber device, the centrifugal device rotates around a shaft to separate the ingredients of the biological fluid; separated ingredients are extruded when the centrifugal device rotates, and the separated ingredients are distributed in a vessel holding separated samples through a fluid passage device; and when a target ingredient occurs, a branch is started to collect trace samples for detecting. The operation process is completed in integrated equipment. The method is applicable to the separation and the detection of ingredients of the biological fluid, including peripheral blood, bone marrow, cell culture fluid, placenta and cord blood.

Description

technical field [0001] The invention relates to a method for processing biological fluid components, in particular to an integrated method for processing biological fluid components, more specifically an integrated method for separating and detecting biological fluid components. Background technique [0002] When separating biological fluid components such as peripheral blood, bone marrow, cell culture fluid, placenta, and umbilical cord blood, they are usually separated by centrifugation by taking advantage of the different densities of the various components contained therein. For example, when separating biological fluid containing hematopoietic stem cells, various components in the sample can be separated by centrifugation to obtain the target separation product. [0003] Some technical solutions are disclosed in the prior art to achieve the mechanization of the above separation process. A chamber connected to a centrifuge as described in US Pat. Various collection met...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/48G01N1/34
Inventor 严整辉
Owner 上海奇康生物科技开发有限公司
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