Membrane nucleic acid amplification sealing reaction tube capable of preloading reagent

Abstract

The invention discloses a membrane nucleic acid amplification sealing reaction tube capable of preloading a reagent. A liquid storage tube body is sleeved with a reaction tube body and sealed at the mouth portion of the reaction tube body, and the mouth portion of the liquid storage tube body is blocked by a seating cap body; a reaction pond capable of containing polymerase liquid is arranged in the reaction tube body, and a liquid storage pond capable of containing buffering liquid is arranged in the liquid storage tube body; the bottom of the liquid storage pond is isolated from the reactionpond through a plastic thin film to prevent the buffering liquid from mixing with the polymerase liquid; the plastic thin film is adopted to perform physical isolation, a user does not need to additionally prepare any other reagent, and operation procedures are greatly simplified; the sealing cover body is not needed to be opened during the whole operation process, and the possibility of aerosolcontamination is effectively avoided; in addition, the preloading of the reagent is achieved while the stability and period of effectiveness of the preloaded reagent are not changed, massive production of all spare parts can be achieved through injection molding, application cost is significantly reduced, and the reaction tube has more significant practical value.

Description

technical field [0001] The invention relates to consumables for nucleic acid amplification experiments in the field of life sciences, in particular to a membrane-type closed reaction tube for nucleic acid amplification that can be preloaded with reagents. Background technique [0002] In the past 20 to 30 years, pathogen diagnostic technology has experienced three technological revolutions, forming a diversified in vitro diagnostic reagent industry technology pattern that currently coexists with clinical biochemical diagnostic technology, immune diagnostic technology and nucleic acid diagnostic technology. [0003] In the late 1980s, with the rise of nucleic acid diagnostic technology represented by Polymerase Chain Reaction (PCR) detection technology, it had an unprecedented impact on clinical diagnosis; especially since the 20th century, constant temperature nucleic acid amplification technology development. [0004] PCR detection technology and constant temperature nucle...

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Problems solved by technology

[0005] The implementation of PCR amplification or constant temperature nucleic acid amplification detection requires biologically active materials such as polymerases, primers, and buffers, but these materials usually need to be stored at -20°C and stored separately. They are prepared and used immediately before detection. It brings a lot of inconvenience to clinical testing applications, such as cumbersome operations and the risk of aerosol contamination during the liquid preparation process; if the polymerase, primers and buffer are premixed, it will bring great benefits to clinical testing applications. convenient

[0006] However, due to biological characteristics, the shelf life and stability of the polymerase, primers, and buffer premixed will be significantly reduced, which will seriously affect the test results; there are also vacuum freeze-drying methods on the market today. However, due to factors such as difficult exploration of process conditions, low yield and long process time, the cost of clinical testing has increased sharply, and it has not been widely used.

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