Carp electrode transforation implantation brain stereotaxic method
A positioning method and electrode technology, applied in medical science, veterinary instruments, sensors, etc., to achieve the effect of reducing mechanical damage and simple operation
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[0042] First, soak the carp in the eugenol aquatic anesthetic solution, then remove the anesthetized carp from the solution, fix it in the brain stereotaxic instrument, clamp the fish mouth horizontally, and gently pull upward to clamp the dorsal fin spine. Clamp the operculum on both sides to fix the 4 feature points to ensure that the fish is in a balanced state and centered.
[0043] Such as figure 1 As shown, the intersection of the midline of the skull surface and the junction of the first fish scale of the head and torso is set as the origin of the coordinate system (3), the midpoint of the upper lip is the lip point (2), and the vertical height H above the lip point is The position at is set as the reference point (1), and the straight line passing through the origin and the reference point is taken as the standard line. The horizontal plane passing through the standard line is defined as the horizontal reference plane, the plane passing through the standard line inclu...
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