35results about How to "Improve digestion efficiency" patented technology

The invention discloses an amino acid phagostimulant for juvenile helichthys nobilis, which mainly comprises aspartic acid, glutamic acid, alanine, glycine, proline and methionine in a content ratio of (4-5): (5-6): (4-5): 3: 2: 1, and the six amino acids are reasonably compounded, so that the phagostimulant for the juvenile helichthys nobilis achieves the best phagostimulant effect on the juvenile helichthys nobilis. The invention also discloses a juvenile mollusca lanuginosa feed based on the amino acid phagostimulant, the juvenile mollusca lanuginosa feed comprises a basal feed and the phagostimulant, and the mass percentage content of the phagostimulant in the juvenile mollusca lanuginosa feed is 0.79-0.96%. The amino acid phagostimulant is added into the basal feed, so that the food-calling fresh fruit of the young mollusca is obviously higher than that of traditional baits, the increase rate of the body weight and the body length of the young mollusca is higher, the survival rate is maximum, and the application prospect is very good.

The invention belongs to the technical field of biogas production equipment, and particularly relates to a kitchen waste anaerobic digestion efficient treatment system, which comprises an anaerobic digestion tank, an exhaust pipe is communicated with one end of the top of the anaerobic digestion tank, and a slag discharge pipe is communicated with the bottom of the anaerobic digestion tank; the pretreatment mechanism is arranged at one end of the top of the anaerobic digestion tank; the garbage is crushed and dehydrated through the design of the pretreatment mechanism, the crushed garbage can be distributed more uniformly in the anaerobic digestion tank, microorganisms can be in full contact with the garbage, the decomposition efficiency of the microorganisms on organic matters in the garbage is improved, and therefore the overall efficiency of anaerobic digestion is improved; the solid concentration is increased, and the relatively high solid concentration is beneficial to forming a tighter reaction system in the anaerobic digestion process, so that the contact area between microorganisms and organic substances is increased, and the anaerobic digestion efficiency is further improved.

The invention discloses microcapsule powder rich in SN-2DHA algal oil and a preparation method of the microcapsule powder rich in SN-2DHA algal oil, and belongs to the technical field of food. The microcapsule powder is prepared from, by mass, 58%-67% of wall materials, 25%-38% of core materials, 0.2%-0.5% of emulsifying agents, 2.1%-3.3% of antioxidants, 0.5%-1% of anti-caking agents, 1%-2% of acidity regulators and 1%-2% of taste regulators, and the wall materials are prepared from cow milk and carbohydrates according to the weight ratio of 1: (1-1.3). Meanwhile, the peroxide value of the algae oil microcapsule powder is also reduced.

The present invention discloses a special dietary beverage and a preparation method thereof that can adjust intestinal flora, belongs to the technical field of special dietary beverage preparation, and includes the following components in parts by weight: 200-400 parts of water, 10-30 parts of oligofructose, 10-30 parts of spirulina powder, 5-20 parts of flaxseed powder, 5-20 parts of wolfberry concentrated juice, 1-5 parts of monk fruit extract, 1-5 parts of inulin, 15-20 parts of whey protein, 5-15 parts of green tea extract, 15-30 parts of selenium-rich protein peptides, 5-20 parts of probiotic microcapsules, 0.3-3 parts of potassium sorbate, 0.3-3 parts of sodium benzoate, 0.1-0.5 parts of citric acid and 0.1-2 parts of sodium ascorbate. The special dietary beverage of the present invention integrates a variety of nutrients and prebiotics, which can promote the growth of intestinal probiotics, regulate blood sugar and reduce cholesterol. The microcapsules prepared by the present invention enhance the colonization ability of probiotics and maintain the balance of intestinal microecology while protecting probiotics from digestive damage. Neomethylhesperidin dihydrochalcone improves the taste, avoids the bitter aftertaste of common sweeteners, and removes the unpleasant taste of metal ion additives.

The invention belongs to the technical field of organoid culture, and particularly relates to tissue digestive juice for organoid culture as well as a preparation method and application of the tissue digestive juice. According to the tissue digestive juice, a DMEM / F-12 culture medium which contains L-glutamine and does not contain 4-hydroxyethyl piperazine ethanesulfonic acid is used as a basic culture medium, calcium chloride, collagenase I type, collagenase III type, V type hyaluronidase, deoxyribonuclease I, 4-hydroxyethyl piperazine ethanesulfonic acid and Y-27632 are combined and have a synergistic effect, cell damage is reduced, meanwhile, the tissue digestive juice has the advantages that the tissue digestive juice can be used for improving the tissue digestive juice quality, and the tissue digestive juice has a good application prospect. The digestion time is shortened, the tissue digestion efficiency is improved, and the culture success rate of the organoid is improved.

The invention relates to the technical field of animal feed, in particular to fermented rapeseed meal as well as a preparation method and application thereof. The present invention provides a fermented rapeseed meal preparation method, which comprises: mixing a rapeseed meal-containing fermentation raw material, an enzyme preparation and a first fermentation bacterial agent, carrying out aerobic fermentation, and after the aerobic fermentation is completed, adding a second fermentation bacterial agent to carry out anaerobic fermentation, the first fermentation inoculant comprises aspergillus niger, aspergillus oryzae, bacillus subtilis and bacillus amyloliquefaciens; the second fermentation microbial agent comprises candida utilis and lactobacillus plantarum. According to the method, the content of toxins and antinutritional factors in the rapeseed meal is effectively reduced, meanwhile, the content of crude protein in the fermented rapeseed meal and the digestion efficiency and metabolizable protein content of nutrient substances such as dry matter and protein in rumen are remarkably improved, and the fermented rapeseed meal is good in palatability, can be used as a good soybean meal substitute and has a good application prospect.

The invention discloses a geological sample digestion instrument temperature measuring device, and relates to the technical field of digestion instruments, the geological sample digestion instrument temperature measuring device comprises a digestion instrument body, a box cover and a sample test tube, the outer surface of the box cover is provided with a display screen, the bottom of the outer surface of the box cover is fixedly connected with a hinge piece through a bolt, and the box cover is hinged to the digestion instrument body through the hinge piece; a stirring assembly is arranged in the box cover and comprises an electric telescopic rod fixedly connected to the upper surface of the box cover, and the telescopic end of the electric telescopic rod is fixedly connected with a fixing frame. The first rotating block drives the temperature measuring rod to rotate, so that the temperature measuring rod inserted into the digestion solution stirs the digestion solution, the digestion solution and the geological sample are fully mixed, uneven heating caused by sedimentation of the geological sample is prevented, it is guaranteed that the geological sample can be fully heated in the digestion solution, and it can also be guaranteed that the geological sample can be effectively digested; the problem of incomplete digestion caused by sedimentation and accumulation of the geological sample is prevented, and the digestion efficiency is improved.

The utility model discloses chemical fiber micro-plastic digestion equipment in the technical field of chemical fiber micro-plastic digestion, which comprises a digestion cylinder, a stirring component is mounted in the digestion cylinder, the stirring component comprises a stirring blade, a stirring frame and a driving motor, the stirring blade and the stirring frame are used for stirring, the driving motor is used for driving the stirring blade and the stirring frame, and a taking component is mounted at the bottom of the digestion cylinder. The taking assembly comprises a filter cartridge used for placing articles, a sealing plate used for placing the filter cartridge, and a moving rod and a bayonet lock used for fixing the sealing plate, compared with the prior art, the device disclosed by the utility model has the beneficial effects that the device is convenient for personnel to mount and dismount the filter cartridge, the mounting and dismounting efficiency is improved, the digestion efficiency is improved, and the working efficiency is improved. Meanwhile, impurities in the filter cylinder can be conveniently cleaned by personnel, raw materials in the digestion cylinder can be stirred, the digestion efficiency of the raw materials is improved, and when the raw materials are added, the raw materials can be scattered, so that the problems that the contact range of an alkaline solution and the raw materials is small and the digestion efficiency is low due to accumulation of the raw materials are solved.

The invention discloses a method for expressing a specific domain of a Mouse SPO11 protein. The method comprises the following steps: using a sequence of the Mouse SPO11 protein as a template, designing primers for PCR amplification, and obtaining a Mouse SPO11 gene fragment after PCR amplification; in an Alp_PET_StHis_SUMO empty vector, sequentially designing a Strep tag II affinity tag, an 8x his affinity tag, and a SUMO solubilizing tag at the N-terminus of a multiple cloning site; subjecting the Mouse SPO11 gene fragment after PCR amplification to gel electrophoresis, followed by gel excision recovery, and then simultaneously subjecting the Mouse SPO11 gene fragment to BamHI and EcoRI double enzyme digestion with the expression vector Alp_PET_StHis_SUMO, and then subjecting the Mouse SPO11 gene fragment to gel excision recovery, connection, transformation, colony PCR, colony culture, and plasmid extraction to obtain a successfully constructed plasmid; inducing expression of the plasmid to obtain an expressed bacterial solution; and purifying the expressed bacterial solution to obtain the Mouse SPO11 protein specific domain. The invention can express the specific structural domain of Mouse SPO11 protein and obtain high-purity Mouse SPO11 protein.

The invention belongs to the field of veterinary drugs, and provides a compound preparation for promoting rumination of ruminants, which is prepared from the following medicaments by weight: 4-7 g of veratrum nigrum, 0.1-0.2 g of yucca extract, 0.05-0.1 g of concentrated sodium chloride, 0.1-0.3 g of bighead atractylodes rhizome, 0.2-0.5 g of pericarpium citri reticulatae, 0.2-0.5 g of medicated leaven and 0.6-1.2 g of yeast culture. The preparation method comprises the following steps: selecting veratrum nigrum roots or whole herbs with roots, cleaning and drying bighead atractylodes rhizome and dried orange peel, carrying out wall breaking treatment, removing large or small particles through a screen to ensure uniform particle size, mixing all the medicaments, granulating, drying and finishing the granules, mixing with superfine silica powder and sodium stearate, and uniformly mixing, thereby obtaining the drug for treating the hyperlipidemia, hyperlipidemia, hyperlipidemia, hyperlipidemia, hyperlipidemia and hyperlipidemia. Finally, tabletting and coating are conducted through a tabletting machine and a coating machine, the overall preparation process is completed after packaging, the number and vitality of microorganisms in the rumen are improved through metabolites of the yeast culture, the digestion and utilization efficiency of the feed is improved, rumen peristalsis is increased through all the Chinese herbal medicine components, and digestive juice secretion is promoted.

The utility model provides a functional cow feed batching device in extremely cold areas, which relates to the technical field of batching devices and comprises a batching tank, four feed holes are formed in the top of the batching tank close to the edge at equal intervals, and a round cavity is formed in the inner wall of the top of the batching tank. And a rotating column penetrates through the circular cavity and the feeding hole and is rotationally connected with the circular cavity through a bearing. The batching quality and efficiency can be improved through the heating ring plate, prepared materials are classified and placed into the crushing cover, and the driving motor drives the rotating column to rotate; a rotating column rotates to drive a rotating rod to rotate through a first bevel gear, a second bevel gear, a rotating column, a rotating rod, a third bevel gear and a fourth bevel gear, the rotating rod rotates to drive a crushing blade to rotate, the effect of crushing the feed can be achieved, the crushed feed can enter a batching tank to be stirred and batched, and the feed can be conveniently mixed. The batching efficiency can be improved, and meanwhile, the feed digestion efficiency can also be improved.

The invention provides a detection pretreatment method of lepidolite ore, a detection method of lithium, magnesium, potassium, sodium and calcium, application and a digestion container, and relates to the technical field of analysis and detection.The detection pretreatment method of the lepidolite ore comprises the following steps that a sample to be detected and an acid digestion agent are mixed, and a mixture is obtained; sequentially carrying out digestion, acid removal and volume metering to obtain a to-be-detected sample solution; wherein the digestion pressure is greater than the standard atmospheric pressure, and the pressure intensity in the digestion container is increased before digestion so as to increase the digestion reaction pressure, so that the digestion and detection efficiency is improved, and a large amount of acid digestion agent is not required to be used; the problems of long detection pretreatment time, high acid consumption and high analysis cost of the lepidolite ore are solved.

The invention is suitable for the technical field of big data mining, and particularly relates to a rule-based execution plan generation method and device, equipment and a medium. The method comprises the steps of evaluating all predicates in a rule set according to a to-be-executed data set to obtain a predicate evaluation result, sorting all predicates according to the predicate evaluation result to obtain a predicate sorting result, performing execution tree construction on all to-be-executed rules in the rule set according to the predicate sorting result to obtain an execution tree, and executing the execution tree according to the execution tree. And according to the to-be-executed data set, evaluating each connection edge of all the execution trees to obtain an edge evaluation result, and according to the edge evaluation result, sorting the execution sequence of the nodes of the execution trees to obtain a rule execution plan corresponding to the execution trees. According to the method, the execution sequence of the predicates and the to-be-executed rules is optimized, so that entity resolution is performed based on the execution plan, the to-be-executed data set can be processed more efficiently, unnecessary calculation and resource consumption are reduced, and the efficiency of entity resolution is improved.

The utility model relates to the technical field of cell culture, and discloses a cell shake flask capable of improving tissue digestion efficiency, which comprises a culture flask body, a flask cover is mounted at the top end of the culture flask body, and a first constant-temperature heating cover is arranged on one side of the outer side wall of the culture flask body. Mounting blocks are fixed to the front end and the rear end of one side of the first constant-temperature heating cover correspondingly, inner cavities are formed in the first constant-temperature heating cover and the second constant-temperature heating cover correspondingly, and electric heating wires are mounted in the inner cavities. When the culture bottle body disclosed by the utility model is used for culturing cells, the culture bottle body can be heated according to actual conditions by arranging components such as the first constant-temperature heating cover, the mounting block, the mounting groove and the second constant-temperature heating cover, and meanwhile, the digestion efficiency of cell tissues can be promoted by heating the culture bottle body; and the culture bottle body can be detached when not needed subsequently, so that the observation of the condition of cells in the culture bottle body through the culture bottle body is not influenced, and the use effect is better.

The application provides a composition for improving intestinal digestive enzyme activity and application thereof, and belongs to the technical field of animal medicine. The composition is composed of double-kidney polysaccharide, ginsenoside Rb1 and ginsenoside Rg1. In the composition, the mass ratio of the double-kidney polysaccharide, the ginsenoside Rb1 and the ginsenoside Rg1 is 90:5:5 or 95:2.5:2.5, preferably 90:5:5, and the intestinal digestive enzyme is specifically duodenal trypsin and amylase. Experimental results show that the composition can significantly synergistically improve the activities of the duodenal trypsin and amylase of laying hens, improve the feed utilization rate, thereby improve the egg production rate, improve the egg weight and the eggshell strength, and effectively improve the breeding benefit of the laying hens.

The invention discloses an extraction method of primary breast cancer tumor related fibroblasts, and belongs to the technical field of cell biology. The method provided by the invention can effectively solve the problems of long extraction time, easy pollution, small number of obtained cells and the like of the primary breast cancer tumor-related fibroblasts in the prior art, and can effectively improve the culture efficiency of the primary breast cancer tumor-related fibroblasts. In addition, by changing the formula of the digestive juice, the extraction time of primary cells is shortened, the pollution risk is reduced, meanwhile, the digestion efficiency is improved, the quantity of obtained cells is large, 0-generation cells can be placed in a T25 culture bottle, passage can be carried out in about 7 days, the culture period is shortened, and the method has important value for the economical efficiency of experimental research.

The invention relates to the technical field of feed preparation, in particular to a bacterium-enzyme combined step-by-step fermentation residue feed and a preparation method and application thereof.The preparation method of the residue feed comprises the steps that fermentation raw materials, bran and soybean meal are mixed according to the mass ratio of 7.5-8.5: 1-1.5: 0.5-1, and a fermentation culture medium is obtained; the method comprises the following steps: mixing a fermentation culture medium with water, firstly adding a compound enzyme to degrade dietary fibers for 2-4 hours, and then adding compound bacteria to ferment for 72-96 hours so as to convert a dietary fiber degradation product into crude protein, thereby obtaining the grain residue feed, the mass ratio of the fermentation culture medium to the compound enzyme to the compound bacteria is 1000: (0.5-1.5): (0.5-2.5). Compared with a one-step fermentation method, the vinasse feed obtained by a two-step method through combination of bacteria and enzymes has the advantages that crude fibers are reduced by 19.65% and crude proteins are improved by 18.98% after 72 hours of fermentation, so that the fattening effect of beef cattle and sheep can be promoted.

The invention provides a soil heavy metal content detection device for environmental detection, and relates to the technical field of soil sample preparation and test.The soil heavy metal content detection device comprises a detection device body, the detection device body comprises a sample preparation table, a digestion tank, a crushing assembly and a stirring mechanism, the bottom of the sample preparation table is in threaded connection with a driving base, and a rotating disc is welded to the top of the driving base; a digestion groove is formed in the bottom of the rotary disc, the digestion groove is embedded into the surface of the rotary disc, a sampling assembly is inserted into one end of the inner side of the digestion groove, the sampling assembly and the digestion groove are of a separable structure, and a stirring mechanism is in threaded connection with the surface of the sample preparation table. The digestion efficiency is improved, meanwhile, the quantitative process can be rapidly completed, more digestion solution samples can be prepared at a time, the safety during bottom stirring is higher, the digestion solution samples are taken more uniformly every time, and the error value of multiple measurements is reduced.

The invention belongs to the field of river crab breeding, and particularly relates to an overwintering breeding method for juvenile crab breeding in saline-alkali soil. An arch-shaped or triangular top design is adopted, a polyethylene plastic film is covered, and the temperature of the water body is controlled within the range of 22-28 DEG C; the humidity and air quality management system comprises a humidifier and an air circulation device and is used for adjusting the humidity in the greenhouse to be between 60% and 80% and keeping air circulation; the illumination system comprises a natural light utilization device and an auxiliary light source and is used for providing sufficient illumination to promote the growth of the juvenile crabs, and the water quality management system comprises a pH value and dissolved oxygen monitor and water quality adjusting equipment and is used for keeping the pH value of a water body to be 6.8-8.5. The pH value in water is kept within 6.8-8.5 through proper water quality adjustment (such as adding of improvers such as lime and sodium sulfate), environmental stress is reduced, stress reaction of juvenile crabs caused by inadaptation of water quality is reduced, and healthy growth of the juvenile crabs is promoted.

The invention discloses a special meal beverage capable of adjusting intestinal flora and a preparation method of the special meal beverage, and belongs to the technical field of special meal beverage preparation. Comprising the following components in parts by weight: 200-400 parts of water, 10-30 parts of fructo-oligosaccharide, 10-30 parts of spirulina powder, 5-20 parts of flaxseed powder, 5-20 parts of fructus lycii concentrated juice, 1-5 parts of a fructus momordicae extract, 1-5 parts of inulin, 15-20 parts of whey protein, 5-15 parts of a green tea extract, 15-30 parts of selenium-enriched protein peptide, 5-20 parts of probiotic microcapsules, 0.3-3 parts of potassium sorbate and 0.3-3 parts of sodium benzoate. 0.1 to 0.5 part of citric acid and 0.1 to 2 parts of sodium ascorbate. The special dietary beverage disclosed by the invention integrates various nutritional ingredients and prebiotics, and can promote the growth of intestinal probiotics, regulate blood sugar and reduce cholesterol. The microcapsule prepared by the invention enhances the colonization ability of the probiotics and maintains the microecological balance of intestinal tracts while protecting the probiotics from being digested and damaged. The neohesperidin dihydrochalcone improves the mouth feel, avoids the lingering bitterness of a common sweetening agent, and also removes the bad taste of a metal ion additive.

This invention relates to a method for detecting metal elements in electronic-grade boron-aluminum doped source samples. The method includes microwave digestion of the sample and inductively coupled plasma atomic emission spectrometry (ICP-AES) to detect the metal element content. By optimizing sample pretreatment and instrument detection conditions, this invention achieves high-precision determination of multiple metal elements in electronic-grade boron-aluminum doped sources. The accuracy, precision, and sensitivity of the detection results meet the requirements for ultra-trace detection of semiconductor-grade and photovoltaic-grade boron-aluminum doped sources.

According to the application of the soft-shelled turtle peptide in promoting protein absorption, the soft-shelled turtle peptide is added into a special medical protein component for the first time, the digestion efficiency of the special medical protein component is remarkably improved, and protein absorption and utilization are promoted. The protein content of the soft-shelled turtle peptide formula product prepared by introducing the soft-shelled turtle peptide as one of protein sources of a protein component is greater than 70g / 100g, the amino acid score belongs to high-quality protein, and the true digestibility is 1.236 times that of a control group and is 1.08 times that of a basic formula without adding the soft-shelled turtle peptide.

The invention discloses an ecological bag suitable for ecologicalization transformation of the bottom of a soil ditch, the ecological bag comprises an ecological planting bag, an ecological anaerobic bag and an ecological bag fixing piece used for fixing the ecological planting bag and the ecological anaerobic bag, and the invention further discloses a construction method for ecologicalization transformation of the bottom of the soil ditch through the ecological bag. According to the ecological bag and the construction method, the ecological transformation difficulty of the ditch bottom of a common farmland soil drainage ditch can be effectively reduced, the water flow height is reasonably controlled, the retention time and disordered flow of drainage in the drainage ditch are promoted, and the in-situ digestion efficiency of farmland water return agricultural non-point source pollution is improved.

The invention provides a bubbling type quick lime digestion device and a digestion method. The device comprises a hollow base and a cylindrical reactor fixedly arranged at the center of the hollow base, in the upward direction of the hollow base, a product collecting tank and at least two overflow circular rings are annularly arranged on the side surface of the cylindrical reactor; the diameter of the product collecting groove is larger than that of the adjacent overflow circular ring, and the diameters of the overflow circular rings are gradually reduced in the direction of the product collecting groove. Limestone is digested in a bubbling mode, continuous digestion can be achieved, calcium hydroxide with the high specific surface area is obtained, and therefore the digestion efficiency is improved, energy consumption and pollution are reduced, operation is convenient, production efficiency is high, and the efficient uniform mixing capacity is achieved; the problems that in the prior art, blocky quick lime is buried in slaked lime powder, and unreacted particle impurities are doped in a finished product are solved.

The present application belongs to the technical field of total phosphorus determination in water quality testing, and specifically discloses a method and system for microfluidic detection of total phosphorus water quality by eliminating the interference of impurities. The method comprises: (1) calibrating with a standard solution to obtain a standard curve; (2) testing a water sample to be tested, including: testing a reference signal with pure water I 0; The water sample to be tested and the first oxidant are sequentially transmitted to the digestion colorimetric module to obtain a digestion signal I 水1 ; Determine whether to perform a second digestion, and if necessary, perform a second digestion; Use a reducing agent to test the digestion signal I 水2 ; Use colorimetric reagent to test digestion signal I 水3 ; Clean and digest the colorimetric module; Use pure water to test the reference signal I 水4 ; Calculate the water sample test results based on the test signal and the standard curve. The application adopts a secondary digestion method, which can not only effectively remove chromatic interference, but also effectively oxidize organic phosphorus compounds that are difficult to oxidize, thereby improving the accuracy of total phosphorus testing in water samples with more complex working conditions.

The utility model discloses a quick and safe lime digestion box which comprises a main body, a water tank is installed on one side of the main body, electric push rods are installed on the two sides of the top end of the main body, the output ends of the electric push rods are connected with a lifting plate, a grinding motor is installed in the middle of the top of the lifting plate, and the output end of the grinding motor is connected to one end of a rotating shaft. And the other end of the rotating shaft penetrates through the lifting plate and the top wall surface of the main body and is connected with a grinding block, and a grinding plate is arranged on the lower side of the grinding block. The lime slaking device relates to the technical field of lime slaking, the lime slaking operation is carried out in the main body, the condition of flying in the lime feeding process can be prevented, so that the health of surrounding personnel is ensured, the grinding block is matched with the grinding plate, the caked lime is crushed, and the slaking efficiency is improved.

The invention discloses a sludge treatment separation tower which comprises a tower body structure, a detection structure is arranged on the peripheral side of the tower body structure, a feeding structure is arranged at the top end of the tower body structure and provided with a stirring structure in a matched mode, the tower body structure comprises a tank body, a temperature assembly, a tank opening, a motor and a transmission gear, and the detection structure comprises a pipeline, a first connector, a second connector and a pH detector. The feeding structure comprises a transmission rod body, a blocking rod, a driven gear ring, a filling hole and a grid plate, and the stirring structure comprises stirring blades, a conical bottom plate and a connecting pin. The sludge treatment device has the beneficial effects that sludge at the bottom in the tower body structure can be stirred and conveyed to the top end in the tank body in a stirring manner through the arranged stirring structure, so that the sludge at the bottom can be circulated to the top end, sludge solidification can be prevented, sludge layering reaction of different layer sections can be prevented, the sludge of the different layer sections can fully react, and the sludge treatment effect is improved. And anaerobic bacteria can be conveniently cultivated.

The invention discloses a yak fallopian tube epithelial cell separation method, and relates to the technical field of cell separation culture. According to the method, collagenase and Accutase are combined for use, so that the cell digestion process becomes milder and more efficient, and the problems that the cell surface antigen is damaged by pancreatin digestion, so that the separated cells cannot maintain the integrity of the cell antigen, the cell differentiation is influenced and the like are solved. When collagenase and Accutase are combined to hydrolyze collagen of an extracellular matrix, DNase I is added, so that DNA released in the cell separation process can be effectively reduced, the cell viscosity and agglutination are reduced, the cell separation efficiency is improved, the digestion efficiency is promoted to be improved, and the activity and adherence of cells are greatly improved.

This invention provides a method for determining 17 elements in coal tar pitch using inductively coupled plasma optical emission spectrometry (ICP-OES), comprising the following three steps: 1) ashing; 2) acidification; and 3) instrumental testing. This invention utilizes a novel high-purity quartz ashing dish with a spout, eliminating the need for expensive platinum dishes compared to existing testing standards. It also overcomes the problem of commonly used laboratory ashing dishes easily introducing impurities such as calcium, magnesium, and aluminum (the main components of ashing dishes and glazes). Furthermore, it reduces the number of main steps, simplifies the process, and improves digestion efficiency by optimizing the ratio of various acids in the digestion system. Based on inductively coupled plasma optical emission spectrometry (ICP-OES), this invention achieves the simultaneous detection of 17 elements in coal tar pitch, and compared to existing technologies, it can also detect lithium and silicon.

The invention discloses a method for treating steel slag by utilizing an ironmaking system, which comprises the following steps: S1, placing the steel slag above a blast furnace tapping channel, and preheating the steel slag by utilizing waste heat; s2, 20-30 minutes after the slag iron is discharged from the blast furnace, the preheated steel slag is uniformly added into liquid slag iron generated in the process of discharging the slag iron from the blast furnace, and molten mixed slag is formed in the main iron runner section; in the step S2, the steel slag adding position is located in a main iron runner section 2-4 m away from a blast furnace taphole. The method for treating the steel slag by using the ironmaking system can simultaneously solve the problems of low stability and low gelation activity of the steel slag, does not add new procedures, is simple in process and low in cost, and opens up a new way for resource utilization of the steel slag.