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A method capable of prolonging the storage time of edible fungus solid strains

A technology of solid strains and storage time, applied in botany equipment and methods, applications, horticulture, etc., can solve the problems of short storage time, general adaptability, and difficulty in storage, so as to prolong storage time, reduce reproduction speed, The effect of strong adsorption capacity

Active Publication Date: 2020-07-17
山东恒发生物科技有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] The current cultivation of edible fungi is generally difficult in prolonging the storage time of the strains, and the adaptability is general. For example, Chinese patent 2010102918274 discloses a preparation method of edible fungus culture medium, which mainly uses crop straw and fresh potato dregs as raw materials. It solves the problem of reusing straw and fresh potato dregs, and provides high-quality edible fungus culture medium, but the storage time is extremely short, and it needs to be stored in a specific environment
[0004] According to the above technical scheme, the disadvantages of the current edible fungus solid strains in storage are: they need to be refrigerated at 4-10°C, and they need to be renewed once within 1-2 months. They are not easy to preserve, the storage time is short, and they are prone to mutation.

Method used

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  • A method capable of prolonging the storage time of edible fungus solid strains
  • A method capable of prolonging the storage time of edible fungus solid strains
  • A method capable of prolonging the storage time of edible fungus solid strains

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] In this embodiment, Pleurotus ostreatus is used for cultivation and a control group is set.

[0030] The solid medium of this embodiment is composed of the following components: trehalose 20g / L, yeast powder 2g / L, peptone 1g / L, potassium dihydrogen phosphate 2g / L, magnesium sulfate 1.5g / L, agar 15g / L, graphite oxide ene 0.01g / L, N-butylpyridine hexafluorophosphate 0.1g / L, sodium carboxymethylcellulose 0.1g / L, polyacrylamide 0.2g / L, nano calcium carbonate 1.3g / L, lauryl sulfonate 0.2 g / L of sodium bicarbonate, the balance is pure water, and the pH value of the culture medium is adjusted to 5-6.

[0031] Among them, nano-graphene oxide has a purity of ≥99%, a sheet thickness of 0.5-2nm, and a sheet length of 500-900nm.

[0032] The production process of the culture medium includes:

[0033] Step S1, preparation of graphene oxide aqueous solution: disperse graphene oxide, sodium lauryl sulfonate, and N-butylpyridine hexafluorophosphate ultrasonically for 5 minutes accord...

Embodiment 2

[0046] In this embodiment, Pleurotus eryngii is used for cultivation and a control group is set.

[0047] The solid medium in this example consists of the following components: trehalose 25g / L, yeast powder 6g / L, peptone 2g / L, potassium dihydrogen phosphate 1g / L, magnesium sulfate 2g / L, agar 20g / L, graphene oxide 1.5g / L, N-butylpyridine hexafluorophosphate 0.85g / L, sodium carboxymethylcellulose 0.5g / L, polyacrylamide 0.8g / L, nano calcium carbonate 9.5g / L, lauryl sulfonic acid Sodium is 0.8g / L, the balance is pure water, and the pH value of the medium is adjusted to 5-6.

[0048] Among them, nano-graphene oxide has a purity of ≥99%, a sheet thickness of 0.5-2nm, and a sheet length of 500-900nm.

[0049] The production process of the culture medium includes:

[0050] Step S1, preparation of graphene oxide aqueous solution: disperse graphene oxide, sodium lauryl sulfonate, and N-butylpyridine hexafluorophosphate ultrasonically for 5 minutes according to parts by weight;

[005...

Embodiment 3

[0064] In this embodiment, Flammulina velutipes was used for cultivation and a control group was set.

[0065] The solid medium in this example consists of the following components: trehalose 22g / L, yeast powder 4g / L, peptone 1.5g / L, potassium dihydrogen phosphate 1.5g / L, magnesium sulfate 1.8g / L, agar 18g / L, Graphene oxide 1g / L, N-butyl pyridine hexafluorophosphate 0.5g / L, sodium carboxymethylcellulose 0.3g / L, polyacrylamide 0.5g / L, nano calcium carbonate 5g / L, lauryl sulfonate 0.5g / L of sodium bicarbonate, the balance is pure water, and the pH value of the culture medium is adjusted to 5-6.

[0066] Among them, nano-graphene oxide has a purity of ≥99%, a sheet thickness of 0.5-2nm, and a sheet length of 500-900nm.

[0067] The production process of the culture medium includes:

[0068] Step S1, preparation of graphene oxide aqueous solution: disperse graphene oxide, sodium lauryl sulfonate, and N-butylpyridine hexafluorophosphate ultrasonically for 5 minutes according to p...

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Abstract

The invention relates to a method for prolonging storage time of edible fungus solid spawn. A solid culturing medium is prepared from, 20-25 g / L of trehalose, 2-6 g / L of yeast powder, 1-2 g / L of peptone, 1-2 g / L of monopotassium phosphate, 1.5-2 g / L of magnesium sulfate, 15-20 g / L of agar, 0.01-1.5 g / L of oxidized graphene, 0.1-0.85 g / L of N-butylpyridine hexafluorophosphate, 0.1-0.5 g / L of sodiumcarboxymethylcellulose, 0.2-0.8 g / L of polyacrylamide, 1.3-9.5 g / L of nano calcium carbonate, 0.2-0.8 g / L of laurinol sodium sulfonate, and the balance purified water. The pH value of the culturing medium is adjusted to 5-6. The method can prolong the storage time of the edible fungus solid spawn, hydroxyl groups and epoxy groups are randomly distributed on an oxidized graphene single sheet, in awater environment, partial hydroxyl groups and partial epoxy groups chemically react with hydroxymethyl cellulose, polyacrylamide and the nano calcium carbonate to form a compound even a polymer so as to reduce the activity of the culturing medium, and edible fungus can survive in a lower activity environment to prolong the storage time.

Description

technical field [0001] The invention relates to the technical field of edible fungus solid strain storage, in particular to a method capable of prolonging the storage time of edible fungus solid strain. Background technique [0002] China has a long history of cultivating edible fungi with a wide variety. The common ones are: shiitake mushrooms, straw mushrooms, mushrooms, fungus, white fungus, Hericium erinaceus, russula, boletus, etc. Edible fungi are delicious, nutritious, and rich in amino acids , can reduce cholesterol and high blood pressure symptoms, and the latest medical research shows that it also has anti-cancer effects, so the development of edible fungus breeding industry is a relatively promising industry. [0003] The current cultivation of edible fungi is generally difficult in prolonging the storage time of the strains, and the adaptability is general. For example, Chinese patent 2010102918274 discloses a preparation method of edible fungus culture medium, w...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01G18/20
CPCA01G18/20
Inventor 赵敬聪李晓博李长田刘孝利高霞王琦崔慧
Owner 山东恒发生物科技有限公司
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