A method capable of prolonging the storage time of edible fungus solid strains
A technology of solid strains and storage time, applied in botany equipment and methods, applications, horticulture, etc., can solve the problems of short storage time, general adaptability, and difficulty in storage, so as to prolong storage time, reduce reproduction speed, The effect of strong adsorption capacity
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Embodiment 1
[0029] In this embodiment, Pleurotus ostreatus is used for cultivation and a control group is set.
[0030] The solid medium of this embodiment is composed of the following components: trehalose 20g / L, yeast powder 2g / L, peptone 1g / L, potassium dihydrogen phosphate 2g / L, magnesium sulfate 1.5g / L, agar 15g / L, graphite oxide ene 0.01g / L, N-butylpyridine hexafluorophosphate 0.1g / L, sodium carboxymethylcellulose 0.1g / L, polyacrylamide 0.2g / L, nano calcium carbonate 1.3g / L, lauryl sulfonate 0.2 g / L of sodium bicarbonate, the balance is pure water, and the pH value of the culture medium is adjusted to 5-6.
[0031] Among them, nano-graphene oxide has a purity of ≥99%, a sheet thickness of 0.5-2nm, and a sheet length of 500-900nm.
[0032] The production process of the culture medium includes:
[0033] Step S1, preparation of graphene oxide aqueous solution: disperse graphene oxide, sodium lauryl sulfonate, and N-butylpyridine hexafluorophosphate ultrasonically for 5 minutes accord...
Embodiment 2
[0046] In this embodiment, Pleurotus eryngii is used for cultivation and a control group is set.
[0047] The solid medium in this example consists of the following components: trehalose 25g / L, yeast powder 6g / L, peptone 2g / L, potassium dihydrogen phosphate 1g / L, magnesium sulfate 2g / L, agar 20g / L, graphene oxide 1.5g / L, N-butylpyridine hexafluorophosphate 0.85g / L, sodium carboxymethylcellulose 0.5g / L, polyacrylamide 0.8g / L, nano calcium carbonate 9.5g / L, lauryl sulfonic acid Sodium is 0.8g / L, the balance is pure water, and the pH value of the medium is adjusted to 5-6.
[0048] Among them, nano-graphene oxide has a purity of ≥99%, a sheet thickness of 0.5-2nm, and a sheet length of 500-900nm.
[0049] The production process of the culture medium includes:
[0050] Step S1, preparation of graphene oxide aqueous solution: disperse graphene oxide, sodium lauryl sulfonate, and N-butylpyridine hexafluorophosphate ultrasonically for 5 minutes according to parts by weight;
[005...
Embodiment 3
[0064] In this embodiment, Flammulina velutipes was used for cultivation and a control group was set.
[0065] The solid medium in this example consists of the following components: trehalose 22g / L, yeast powder 4g / L, peptone 1.5g / L, potassium dihydrogen phosphate 1.5g / L, magnesium sulfate 1.8g / L, agar 18g / L, Graphene oxide 1g / L, N-butyl pyridine hexafluorophosphate 0.5g / L, sodium carboxymethylcellulose 0.3g / L, polyacrylamide 0.5g / L, nano calcium carbonate 5g / L, lauryl sulfonate 0.5g / L of sodium bicarbonate, the balance is pure water, and the pH value of the culture medium is adjusted to 5-6.
[0066] Among them, nano-graphene oxide has a purity of ≥99%, a sheet thickness of 0.5-2nm, and a sheet length of 500-900nm.
[0067] The production process of the culture medium includes:
[0068] Step S1, preparation of graphene oxide aqueous solution: disperse graphene oxide, sodium lauryl sulfonate, and N-butylpyridine hexafluorophosphate ultrasonically for 5 minutes according to p...
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