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Culture medium for rapid proliferation of neural stem cells

A neural stem cell and culture medium technology, applied in the field of rapid proliferation of neural stem cell culture medium, can solve the problems of unsatisfactory cell proliferation speed, poor cell adhesion, residue, etc., to promote rapid and large proliferation, increase the rate of metabolism, and accelerate absorption The effect of using

Inactive Publication Date: 2017-01-04
ANHUI HUIEN BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0002] The neural stem cell culture medium in the prior art often uses fetal bovine serum. Due to the use of animal-derived fetal bovine serum, the stem cell culture medium is mixed with non-human substances, causing residue or pollution, and the existing neural stem cell culture medium exists Poor cell adhesion and unsatisfactory cell proliferation speed

Method used

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Examples

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Effect test

Embodiment 1

[0015] A culture medium for rapidly proliferating neural stem cells proposed by the present invention, comprising: RPMI-1640 medium, trehalose, recombinant human insulin, human insulin growth factor, endothelial cell growth factor, potassium chloride, amino acid chelated selenium, glutamine , progesterone, coenzyme I, lipoic acid, vitamins, Polygonatum polysaccharide, Codonopsis polysaccharide, ginsenoside.

Embodiment 2

[0017] A culture medium for rapidly proliferating neural stem cells proposed by the present invention, comprising: RPMI-1640 medium, trehalose, recombinant human insulin, human insulin growth factor, endothelial cell growth factor, potassium chloride, amino acid chelated selenium, glutamine , progesterone, coenzyme I, lipoic acid, vitamins, Polygonatum polysaccharide, Codonopsis polysaccharide, ginsenoside.

[0018] Wherein, the concentration of trehalose is 2.5g / L, the concentration of recombinant human insulin is 3g / L, the concentration of human insulin growth factor is 20mg / L, the concentration of endothelial cell growth factor is 25μg / L, and the concentration of potassium chloride is 0.8g / L, the concentration of amino acid chelated selenium is 0.6μg / L, the concentration of glutamine is 50mg / L, the concentration of progesterone is 20mg / L, the concentration of coenzyme I is 80μg / L, and the concentration of lipoic acid is 25mg / L, the concentration of vitamins is 50mg / L, the c...

Embodiment 3

[0020] A culture medium for rapidly proliferating neural stem cells proposed by the present invention, comprising: RPMI-1640 medium, trehalose, recombinant human insulin, human insulin growth factor, endothelial cell growth factor, potassium chloride, amino acid chelated selenium, glutamine , progesterone, coenzyme I, lipoic acid, vitamins, Polygonatum polysaccharide, Codonopsis polysaccharide, ginsenoside.

[0021] Wherein, the concentration of trehalose is 3.5g / L, the concentration of recombinant human insulin is 2g / L, the concentration of human insulin growth factor is 30mg / L, the concentration of endothelial cell growth factor is 15μg / L, and the concentration of potassium chloride is 1.3g / L, the concentration of amino acid chelated selenium is 0.4μg / L, the concentration of glutamine is 80mg / L, the concentration of progesterone is 10mg / L, the concentration of coenzyme I is 200μg / L, and the concentration of lipoic acid is 15mg / L, the concentration of vitamins is 100mg / L, the...

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Abstract

The invention discloses a culture medium for rapid proliferation of neural stem cells. The culture medium is prepared from an RPMI-1640 culture medium, trehalose, recombinant human insulin, insulin growth factors, endothelial cell growth factors, potassium chloride, amino acid chelated selenium, glutamine, progesterone, coenzyme I, lipoic acid, vitamin, polygahatous polysaccharides, polysaccharide from radix codonopsis and ginseng saponin. The culture medium for rapid proliferation of the neural stem cells is good in cell wall adherence, fast in proliferation and free of animal source ingredients and does not produce residues or pollution, meanwhile the anti-pathogen microbial effect of the culture medium can be improved, and the cost of the culture medium can be reduced.

Description

technical field [0001] The invention relates to the technical field of stem cell culture medium, in particular to a culture medium for rapidly proliferating neural stem cells. Background technique [0002] The neural stem cell culture medium in the prior art often uses fetal bovine serum. Due to the use of animal-derived fetal bovine serum, the stem cell culture medium is mixed with non-human substances, causing residue or pollution, and the existing neural stem cell culture medium exists Cell adhesion is poor, cell proliferation rate is not ideal and so on. Contents of the invention [0003] Based on the technical problems existing in the background technology, the present invention proposes a culture medium for rapidly proliferating neural stem cells, which has fast proliferation speed, good cell adhesion, and no animal-derived components, which will not cause residue or pollution, and can improve the culture medium of the culture medium at the same time. The anti-patho...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N5/0797
CPCC12N5/0623C12N2500/12C12N2500/32C12N2500/34C12N2500/38C12N2501/10C12N2501/105C12N2501/30C12N2501/33C12N2501/70C12N2501/90C12N2501/998C12N2501/999
Inventor 张正亮
Owner ANHUI HUIEN BIOTECH
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