Hematopoietic stem cell culture medium

A technology of hematopoietic stem cells and culture medium, applied in the field of cell culture, can solve problems such as poor activity and proliferation ability, and achieve the effect of protecting growth factor activity, continuous release and high purity

Inactive Publication Date: 2019-04-05
金时代进出口贸易重庆有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] Aiming at the above-mentioned deficiencies in the prior art, the present invention provides a hematopoietic stem cell culture medium, which can effectiv

Method used

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  • Hematopoietic stem cell culture medium
  • Hematopoietic stem cell culture medium
  • Hematopoietic stem cell culture medium

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Experimental program
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Embodiment 1

[0036]A kind of hematopoietic stem cell culture medium, comprises basal medium and additive, and basal medium is DMEM culture medium (Beijing Qingda Tianyi Science and Technology Co., Ltd.), and by final concentration, additive comprises the component of following content: trehalose 60ng / mL , Vitamin C 50ng / mL, Berberine 1mg / mL, Stephanine 30μmol / mL, the first cytokine sustained-release sphere 1.2mg / mL, the second cytokine sustained-release sphere 15mg / mL, sodium chloride 3mg / mL , glutathione 20mg / mL, hyaluronic acid 5μg / mL, grape seed extract 2mg / mL, ginsenoside 6mg / mL and tremella polysaccharide 5mg / mL.

[0037] Wherein, the first cytokine sustained-release sphere is prepared by the following method:

[0038] (1) adding dextran and polyethylene glycol into water respectively, and stirring until dissolved to obtain 8wt% dextran aqueous solution and 8wt% polyethylene glycol aqueous solution;

[0039] (2) At 0-4°C, mix dextran aqueous solution and polyethylene glycol aqueous s...

Embodiment 2

[0053] A hematopoietic stem cell culture medium, including basal medium and additives, the basal medium is DMEM medium, and the additives include the following components in terms of final concentration: trehalose 80ng / mL, vitamin C65ng / mL, berberine 3mg / mL, Stephanine 50μmol / mL, the first cytokine sustained-release sphere 2.5mg / mL, the second cytokine sustained-release sphere 30mg / mL, sodium chloride 8mg / mL, glutathione 30mg / mL, hyaluronic acid acid 10μg / mL, grape seed extract 5mg / mL, ginsenoside 12mg / mL and tremella polysaccharide 10mg / mL.

[0054] Wherein, the first cytokine sustained-release sphere is prepared by the following method:

[0055] (1) adding dextran and polyethylene glycol into water respectively, and stirring until dissolved to obtain a 10wt% dextran aqueous solution and a 10wt% polyethylene glycol aqueous solution;

[0056] (2) At 0-4°C, mix dextran aqueous solution and polyethylene glycol aqueous solution at a mass ratio of 1:10, and then add interleukin-...

Embodiment 3

[0070] A hematopoietic stem cell culture medium, including basal medium and additives, the basal medium is DMEM medium, and the additives include the following components in terms of final concentration: trehalose 70ng / mL, vitamin C55ng / mL, berberine 2mg / mL, Stephanine 38μmol / mL, the first cytokine sustained-release sphere 2.0mg / mL, the second cytokine sustained-release sphere 22mg / mL, sodium chloride 4mg / mL, glutathione 25mg / mL, hyaluronic acid acid 6μg / mL, grape seed extract 3mg / mL, ginsenoside 7mg / mL and tremella polysaccharide 6mg / mL.

[0071] Wherein, the first cytokine sustained-release sphere is prepared by the following method:

[0072] (1) adding dextran and polyethylene glycol into water respectively, and stirring until dissolved to obtain a 10wt% dextran aqueous solution and a 10wt% polyethylene glycol aqueous solution;

[0073] (2) At 0-4°C, mix dextran aqueous solution and polyethylene glycol aqueous solution at a mass ratio of 1:8, and then add interleukin-3, i...

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Abstract

The invention discloses a hematopoietic stem cell culture medium. The hematopoietic stem cell culture medium comprises a basal culture medium and an additive. According to final concentration, the additive is prepared from the following components: 60-80 ng/mL of mycose, 50-65 ng/mL of vitamin C, 1-3 mg/mL of berberine, 30-50 [mu]mol/mL of stephanine, 1.2-2.5 mg/mL of first cell factor slow-release sphere, 15-30 mg/mL of second cell factor slow-release sphere, 3-8 mg/mL of sodium chloride, 20-30 mg/mL of glutathione, 5-10 [mu]g/mL of hyaluronic acid, 2-5 mg/mL of grape seed extract, 6-12 mg/mLof ginsenoside and 5-10 mg/mL of tremella polysaccharide. According to the hematopoietic stem cell culture medium, the components are matched reasonably, the specific components interact with each other and can resist pathogenic microorganisms, and survival rate, proliferation speed and activity of the hematopoietic stem cells are improved.

Description

technical field [0001] The invention belongs to the technical field of cell culture, and in particular relates to a culture medium for hematopoietic stem cells. Background technique [0002] Hematopoietic stem cells are the adult stem cells in the blood system, which are a heterogeneous group with the ability of long-term self-renewal and the potential to differentiate into various mature blood cells. It is a type of adult stem cell with the longest and most in-depth research history, and it has important guiding significance for the study of various stem cells, including tumor stem cells. Because hematopoietic stem cells have the potential of multi-directional differentiation, transplanting hematopoietic stem cells into the body is an effective method for the treatment of leukemia, lymphoma and other malignant tumors, metabolic diseases, autoimmune diseases and congenital immune deficiencies that seriously endanger human health. alleviating the suffering of patients. Howe...

Claims

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Application Information

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IPC IPC(8): C12N5/0789
CPCC12N5/0647C12N2500/05C12N2500/30C12N2500/76C12N2500/34C12N2500/38C12N2501/90C12N2501/905C12N2501/998
Inventor 赵翔宋彩霞曹玉昊叶劲涛王仁杰黄启程邱阳李国焱唐玲胡小东
Owner 金时代进出口贸易重庆有限公司
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