The invention provides an anion latex pillow and a preparation method of the anion latex pillow. The preparation method of the anion latex pillow comprises the following steps: preparing anion powder,grinding rare earth, tourmaline, medical stone and gull rock, and removing radioactive substance; preparing latex slurry, mixing natural emulsion, the anion powder, zinc oxide, pure magnesium, polyoxyethylene ether, oleic acid, calcium stearate and IONOLLC antioxidant, carrying out uniform stirring, and adding sodium hydroxide or potassium hydroxide for adjusting the pH to be 8-10, wherein duringthe stirring process, appropriate amount of air is introduced, so that sufficient foaming is realized; preparing wear-resistant layer slurry, mixing the natural emulsion, the anion powder, glass powder, zinc oxide, and calcium chloride, and carrying out uniform stirring; and after injection molding, carrying out gelatinizing forming under 105-115 DEG C, and finally, carrying out demolding washingand heating drying operation. For the preparation method provided by the invention, the forming quality and good health care effects of the anion latex pillow can be guaranteed, meanwhile, due to thesimplified preparation process, the good environmental protection property is obtained.