Slow-release and micro-organism-inhibition type artificial dermis model and construction method thereof

A construction method, artificial technology, applied in pharmaceutical formulations, coatings, pharmaceutical sciences, etc.

Active Publication Date: 2019-07-19
ZHENDE MEDICAL CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the deficiencies in the prior art, the object of the present invention is to provide a slow-release antibacterial artificial dermis model and its construction method. A slow-release layer is added between the support layer and the silica gel film layer. Th

Method used

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  • Slow-release and micro-organism-inhibition type artificial dermis model and construction method thereof
  • Slow-release and micro-organism-inhibition type artificial dermis model and construction method thereof
  • Slow-release and micro-organism-inhibition type artificial dermis model and construction method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0086] Antibacterial agent selects a kind of embodiment-silver nitrate of soluble component for use;

[0087] Step 1, the construction of the slow-release layer:

[0088] Because silver nitrate is a water-soluble inorganic salt, when the slow-release layer meets water, the silver ions become free, and driven by the concentration difference, they penetrate the dense fiber structure of the cellulose film and reach the wound surface to play a role in continuous sterilization. Therefore, in this embodiment, a denser and thicker biocellulose film should be selected to ensure the slow release of silver ions.

[0089] Take the density as 10mg / cm 3 A biological cellulose film with a thickness of about 1 cm is soaked in a 5% silver nitrate solution for 10 hours to fully absorb the silver nitrate. Pre-freeze the expanded biological cellulose membrane at -20°C, then transfer it to a vacuum freeze dryer, freeze-dry for 10 hours, make it incompletely freeze-dried and become a film with a...

Embodiment 2

[0099] Antibacterial agent selects a kind of embodiment-sodium zirconium phosphate of micron-sized particle for use;

[0100] Step 1, the construction of slow-release layer;

[0101] Since the particle size of sodium silver zirconium phosphate is relatively micron, it is necessary to choose a biocellulose membrane with a low density and a moderate thickness. To ensure that the sodium zirconium phosphate silver particles can be evenly adsorbed in the biocellulose membrane. When the biological cellulose membrane changes from the expanded state to the thin film state, the pores of the biological cellulose membrane change from micrometer to nanometer, so that the silver zirconium phosphate particles are wrapped between the biological cellulose membranes. Because the biological fiber has the function of absorbing liquid, when the body fluid enters the slow-release layer, the silver ions loaded by sodium zirconium phosphate enter the body fluid and become free, so that they can ent...

Embodiment 3

[0113] Antibacterial agent selects a kind of embodiment of nano-scale particle-nano-silver particle;

[0114] Step 1, the construction of slow-release layer;

[0115] In this embodiment, nano-silver particles are selected as the antibacterial agent, taking into account the adsorption and release of nano-silver. In this embodiment, a biocellulose film with moderate density and thick thickness is selected as the slow-release layer.

[0116] Take the dry weight as 5mg / cm 3 A wet biological cellulose film with a thickness of about 0.8cm±0.1cm is soaked in a 10% nano-silver suspension for 10 hours to fully absorb the nano-silver. Pre-freeze the biocellulose membrane in the above-mentioned expanded state at -20°C, then transfer it to a vacuum freeze dryer, freeze-dry for 10 hours, make it incompletely freeze-dried and become a thin film with a thickness of about 1mm, so that the silver nanoparticles Encased in cellulose. The biocellulose film was then dried at 30° C. for 24 hour...

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Abstract

The present invention discloses a slow-release and micro-organism-inhibition type artificial dermis model and a construction method thereof. The slow-release and micro-organism-inhibition type artificial dermis model comprises a silica gel membrane, a stent layer arranged under the silica gel membrane, and a slow-release layer arranged between the silica gel membrane and the stent layer. The construction method is as follows: the slow-release layer is selected from a bio-cellulose membrane, the bio-cellulose membrane in an expanded state is prepared into a dense bio-cellulose thin membrane byvacuum drying, at the same time, a micro-organism-inhibition agent is retained inside the bio-cellulose thin membrane, and then the bio-cellulose thin membrane is spread flatly on a bottom part of a mold and freeze-dried together with collagen and other stent components to be integrated together; finally, the integrated material is bonded with the silica gel membrane to form the final model; and adesign of the slow-release layer can load the micro-organism-inhibition agent, also can regulate and control a releasing speed of the micro-organism-inhibition agent, enables the micro-organism-inhibition agent to be stably and evenly released, and avoids infection or poisoning during use of artificial dermis.

Description

technical field [0001] The invention relates to the field of skin tissue engineering, in particular to a slow-release antibacterial artificial dermis model and a construction method thereof. Background technique [0002] The skin wraps the surface of the human body and is in direct contact with the external environment. It is an organ that protects, excretes, regulates body temperature, and senses external stimuli. It is the largest organ in the human body. Various types of skin defect wounds are very common clinically. Currently, the dermal substitutes used for wound treatment mainly include artificial dermis, acellular allogeneic skin, and tissue-engineered artificial dermis containing active cells. Artificial dermis products originally have a wide range of sources, are easy to store and transport, have significant clinical effects and high product safety, and currently occupy a mainstream position in the market. [0003] The artificial dermis used for wound repair usuall...

Claims

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Application Information

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IPC IPC(8): A61L27/40A61L27/20A61L27/24A61L27/02A61L27/50A61L27/54
CPCA61L27/025A61L27/20A61L27/24A61L27/50A61L27/54A61L2300/104A61L2300/404A61L2300/602A61L2300/606A61L2400/12C08L1/02
Inventor 张锐鲁建国王姣
Owner ZHENDE MEDICAL CO LTD
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