Antibacterial poly(ε-caprolactone)/poly(ε-caprolactone)-REDV/gelatin electrospun fiber membrane and preparation method

A technology of electrospinning fiber and caprolactone, which is applied in the field of biomedical materials, can solve the problems of accelerating endothelialization and antibacterial, and achieves the promotion of adhesion and proliferation, good biocompatibility and biodegradability, and broad application prospects. Effect

Inactive Publication Date: 2019-09-06
TIANJIN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the present invention, the poly(ε-caprolactone)-REDV (publication number CN106729976A) synthesized by our research group before is blended with poly(ε-caprolactone) and gelatin for electrospinning, and eugenol is encapsulated at the same time. Electrospun fiber membrane materials with dual functions of endothelialization and antibacterial on the material surface, this work has not been reported so far

Method used

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  • Antibacterial poly(ε-caprolactone)/poly(ε-caprolactone)-REDV/gelatin electrospun fiber membrane and preparation method
  • Antibacterial poly(ε-caprolactone)/poly(ε-caprolactone)-REDV/gelatin electrospun fiber membrane and preparation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0018] The mass fractions of poly(ε-caprolactone), poly(ε-caprolactone)-REDV, and gelatin in the electrospun fibers in this example are 40wt%, 30wt%, and 30wt%, respectively, and eugenol accounts for the total amount of the above polymers 30wt%.

[0019] Weigh 300mg poly(ε-caprolactone) (M n =8×10 4 ), 225mg poly(ε-caprolactone)-REDV (M n =3×10 4 ), 225 mg of gelatin was dissolved in 5 mL of trifluoroethanol, and glacial acetic acid was added dropwise and stirred until the solution was homogeneously clear, then 225 mg of eugenol was added, and mixed uniformly; the solution was placed in a syringe, and a single-channel syringe pump electrospinning device was used. Electrospinning was performed at a voltage of 12kV, a flow rate of 0.6mL / h, and a receiving distance of 18cm. After 8 hours, a fiber membrane with a fiber diameter of 500nm-800nm ​​and a thickness of 50μm was collected.

[0020] figure 1 It shows that the obtained electrospun fibers have good morphology and no de...

Embodiment 2

[0022] The mass fractions of poly(ε-caprolactone), poly(ε-caprolactone)-REDV, and gelatin in the electrospun fibers in this example are 80wt%, 10wt%, and 10wt%, respectively, and eugenol accounts for the total amount of the above polymers 5wt%.

[0023] Weigh 400mg poly(ε-caprolactone) (M n =12×10 4 ), 50mg poly(ε-caprolactone)-REDV (M n =1×10 4 ), 50 mg of gelatin was dissolved in 5 mL of trifluoroethanol, and glacial acetic acid was added dropwise and stirred until the solution was homogeneously clear, then 25 mg of eugenol was added and mixed uniformly; the solution was placed in a syringe, and a single-channel syringe pump electrospinning device was used. Electrospinning was performed under the conditions of voltage of 10kV, flow rate of 0.4mL / h, and receiving distance of 15cm. After 12h, a fiber membrane with a fiber diameter of 200nm-500nm and a thickness of 50μm was collected.

Embodiment 3

[0025] The mass fractions of poly(ε-caprolactone), poly(ε-caprolactone)-REDV, and gelatin in the electrospun fibers in this example are 70wt%, 20wt%, and 10wt%, respectively, and eugenol accounts for the total amount of the above-mentioned polymers 20wt%.

[0026] Weigh 700mg poly(ε-caprolactone) (M n =5×10 4 ), 200mg poly(ε-caprolactone)-REDV (M n =5×10 4), 100 mg of gelatin was dissolved in 10 mL of trifluoroethanol, and glacial acetic acid was added dropwise and stirred until the solution was homogeneously clear, then 200 mg of eugenol was added and mixed uniformly; Electrospinning was performed under the condition of voltage of 15kV, flow rate of 0.8mL / h, and receiving distance of 20cm. After 12h, a fiber membrane with a fiber diameter of 200nm-500nm and a thickness of 100μm was collected.

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Abstract

The invention discloses an antibacterial poly (Epsilon- caprolactone) / poly (Epsilon- caprolactone)-REDV / gelatin electro-spinning fibrous coat and a preparation method thereof; the electro-spinning fibrous coat is formed by poly (Epsilon- caprolactone) / poly (Epsilon- caprolactone)-REDV / gelatin fiber with diameter of 200-1200 nm and a plant source antibacterial agent entrapped at the inner part of fiber; the thickness of the film is 50-150 mu m. The preparation method includes steps of dissolving (Epsilon- caprolactone), poly (Epsilon- caprolactone)-REDV and gelatin in trifluoroethanol; dropwise adding glacial acetic acid until the solution is clear; forming electro-spinning solution; then adding plant source antibacterial agent eugenol; using a single-path injection pump electro-spinning device, and applying a static spinning method to obtain the electro-spinning fibrous coat. The electro-spinning fibrous coat has double functions of accelerating endothelialization of the material surface and preventing bacterial, the fibrous coat has application prospect in terms of artificial blood vessel biomedical materials.

Description

technical field [0001] The invention relates to an antibacterial poly(ε-caprolactone) / poly(ε-caprolactone)-REDV / gelatin electrospun fiber membrane carrying a plant-derived antibacterial agent and a preparation method thereof, belonging to the field of biomedical materials. Background technique [0002] In recent years, cardiovascular disease has become the leading cause of human death. Using electrospinning film forming technology, by adjusting the electrospinning parameters, an electrospun fiber membrane material with a fiber diameter of several hundred nanometers and a large specific surface area can be prepared, which can simulate the extracellular matrix in structure, which is beneficial to cells. Adhesion, proliferation and migration can be used for cardiovascular remodeling. [0003] Poly(ε-caprolactone) has good biocompatibility, biodegradability and excellent mechanical properties, and is a commonly used material in the research of small-caliber artificial blood ves...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): D04H1/4382D04H1/728A61L27/18A61L27/22A61L27/54A61L27/58
Inventor 袁晓燕李珍光周芳周培琼任丽霞赵蕴慧
Owner TIANJIN UNIV
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