Preparation method of copper ion mediated anticoagulant coating with function of in situ catalysis of NO release

An in-situ catalysis, copper ion technology, applied in the field of biomedical engineering functional materials, can solve the problems of complex preparation process, difficult to effectively control the copper ion loading, etc., achieve high research and application value, excellent scavenging of oxygen free radicals, excellent Antioxidant effect

Active Publication Date: 2014-12-17
GUANGZHOU NANCHUANG EVEREST MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Existing methods for loading copper ions include pulling method, dip coating method and ion exchange method, etc. Some of t...

Method used

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  • Preparation method of copper ion mediated anticoagulant coating with function of in situ catalysis of NO release
  • Preparation method of copper ion mediated anticoagulant coating with function of in situ catalysis of NO release
  • Preparation method of copper ion mediated anticoagulant coating with function of in situ catalysis of NO release

Examples

Experimental program
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Effect test

Embodiment 1

[0028] A method for preparing an anticoagulant coating mediated by copper ions with in-situ catalytic NO release function, obtained by the following steps:

[0029] A. Polish, clean and dry the medical stainless steel material that needs to be modified;

[0030] B. Place the sample obtained in step A in a buffer system (acetic acid-acetate buffer) with pH=4, then add dopamine with a concentration of 0.01 mg / ml, collagen with a concentration of 0.01 mg / ml and a concentration of 0.01 mg / ml to the system. 0.01mg / ml CuCl 2 Solution, react at 10°C for 0.5h;

[0031] C. For the sample obtained in step B, immerse it in deionized water, clean it ultrasonically for 3 times, each time for 5min, and then wash it in N 2 The target material can be obtained by drying under the conditions.

Embodiment 2

[0033] A method for preparing an anticoagulant coating mediated by copper ions with in-situ catalytic NO release function, obtained by the following steps:

[0034] A. Polish, clean and dry the nickel-titanium alloy material that needs to be modified;

[0035] B, the sample obtained in step A is placed in a buffer system (Tris-HCl buffer) of pH=7, and then adding epigallocatechin gallate (EGCG) with a concentration of 10 mg / ml to the system, the concentration is 10 mg / ml ml of polyethyleneimine and CuSO at a concentration of 5 mg / ml 4 Solution, react at 30°C for 24h;

[0036] C. For the sample obtained in step B, immerse it in deionized water, clean it ultrasonically for 3 times, each time for 5min, and then wash it in N 2 The target material can be obtained by drying under the conditions.

Embodiment 3

[0038] A method for preparing an anticoagulant coating mediated by copper ions with in-situ catalytic NO release function, obtained by the following steps:

[0039] A. Polish, clean and dry the cobalt-based alloy that needs to be modified;

[0040] B. Place the sample obtained in step A in a buffer system (acetic acid-acetate buffer) with pH=5, and then add epicatechin gallate (ECG) with a concentration of 2 mg / ml to the system at a concentration of 3 mg / ml of butanediamine and a concentration of 1mg / ml of CuCl 2 Solution, at 15°C, react for 4h;

[0041] C. For the sample obtained in step B, immerse it in deionized water, clean it ultrasonically for 3 times, each time for 5min, and then wash it in N 2 The target material can be obtained by drying under the conditions.

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Abstract

The invention discloses a preparation method of a copper ion mediated anticoagulant coating with function of in situ catalysis of NO release. The method includes steps of preparing an acidic buffer solution, and adding a compound with pyrogallol structure and certain concentration, a compound with amino or thiol, and copper ion soluble salt. The method of the invention has the advantages of simple operation, mild reaction conditions and easiness. The prepared coating has the advantages of controllable content of the units containing multiple amino or thiol compounds, and easily controlled loading amount of copper ion. The modified coating prepared by the method has excellent adhesion with a base material, and through act of the copper ions in the coating with the blood, the coating can conduct in situ catalyze the NO donor molecules in blood to continuously decompose and release NO molecules, thus realizing the inhibition of platelet activation and aggregation, inhibiting smooth muscle cell proliferation and migration, and protecting vascular endothelial layer function.

Description

technical field [0001] The invention relates to biomedical engineering functional materials, especially the technical field of preparation of excellent anticoagulant materials. Background technique [0002] After the cardiovascular material is implanted into the human body, plasma proteins will first adhere to the surface of the material, and the adhered fibrinogen, von Willebrand factor, etc. will then trigger the attachment and activation of platelets on the surface of the material, and release various coagulation factors, and then Formation of blood clots. Therefore, the anticoagulant problem of blood-contact materials, such as vascular stents, artificial blood vessels, artificial heart valves, inferior vena cava filters, etc., is a permanent subject of research in the field of biomaterials. The development of materials with excellent anticoagulant properties is also the basic requirement and goal for long-term and stable service of blood-contact materials. Among the ma...

Claims

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Application Information

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IPC IPC(8): A61L33/02A61L33/04A61L33/00A61L33/06
Inventor 王进罗日方刘玉洁黄楠申方瑜赵元聪冷永祥陈俊英
Owner GUANGZHOU NANCHUANG EVEREST MEDICAL TECH CO LTD
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