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Inductive agent for regeneration of periodontium tissue of temperature sensitivity and preparation method thereof

A periodontal tissue regeneration and inducer technology, which is applied in the field of temperature-sensitive periodontal tissue regeneration inducers, can solve the problems of short half-life, easy to be degraded, and large side effects of systemic application, and achieve high porosity structure and strong inhibition of tooth decay. Effects of competence, good surface activity and plasticity around pathogenic bacteria

Inactive Publication Date: 2011-07-27
HOSPITAL ATTACHED TO QINGDAO UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, exogenous growth factors are often applied directly to patients with dental diseases to promote the regeneration of their alveolar bone. However, exogenous growth factors have short half-lives and are easily degraded in the body. Directly applied to patients will also bring rejection of the body, and its systemic application has large side effects

Method used

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  • Inductive agent for regeneration of periodontium tissue of temperature sensitivity and preparation method thereof

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Experimental program
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Embodiment 1

[0029] The purpose of the present invention is to provide a chitosan temperature-sensitive hydrogel composite with chitosan, chitosan quaternary ammonium salt nanoparticles and α, β-sodium glycerophosphate as carriers and carrying plasmids containing BMP-2 sequence It also provides a method for preparing the temperature-sensitive hydrogel complex.

[0030] Chitosan is the only natural cationic polysaccharide in nature. Its structure is similar to that of extracellular matrix glycosaminoglycan GAGs. It has good histocompatibility, biodegradability, antibacterial and non-toxic properties. It can be compounded with a variety of anionic polyelectrolytes in a variety of ways to form a physiological environment temperature-sensitive hydrogel, which can be widely used in tissue engineering scaffolds, drug-carrying systems and cell culture carriers. Due to its cationic properties, chitosan can be used as a non-viral carrier to combine with negatively charged DNA, RNA and oligonucleotides...

Embodiment 2

[0046] The specific steps for preparing the C S / HTCCn(pDNA-BMP2)-GP temperature-sensitive hydrogel complex in this embodiment are as follows:

[0047] (1) First, prepare a solution of chitosan quaternary ammonium salt nanoparticles (HTCCn). The specific quaternization steps are the same as in Example 1.

[0048] (2) Then the prepared chitosan quaternary ammonium salt (HTCC) is prepared by ultrasonic emulsification method to prepare chitosan quaternary ammonium salt nanoparticles (HTCCn). The specific preparation process is the same as in Example 1.

[0049] (3) Prepare the chitosan quaternary ammonium salt nanoparticle solution encapsulating the plasmid. The pT7T3D-PacI plasmid containing the BMP-2 sequence was transferred into Escherichia coli for amplification, and the plasmid was purified after isolation and extraction. The steps were the same as in Example 1.

[0050] The specific encapsulation process in this embodiment is as follows: HTC Cn solution (0.1%, dissolved in 5ml lac...

Embodiment 3

[0053] The C S / HTC Cn(pDNA-BMP2)-GP temperature-sensitive hydrogel complex obtained in the present invention is used as a periodontal tissue inducer in animal experiments and clinical trials. The test results are as follows:

[0054] (1) Establish an animal model of canine periodontal disease, give sub-periosteal injection of CS / HTCCn(pDNA-BMP2)-GP temperature-sensitive hydrogel complex with alveolar bone resorption. X-rays show at 1, 3, and 6 months All have different degrees of bone regeneration.

[0055] (2) Wang Moumou, male, 34 years old. Visited for 3 years due to loose teeth. Clinical examination and X-ray examination diagnosed as "adult periodontitis (severe)". After three months of basic SPR treatment, local CS / HTC Cn(pDNA-BMP2)-GP temperature-sensitive hydrogel complex was injected. X-rays taken at 3 months showed that the alveolar bone was regenerated by about 0.6mm compared to before injection.

[0056] (3) Zhang, female, 28 years old, went to see a doctor because of b...

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Abstract

The invention provides an inductive agent for regeneration of periodontium tissue of temperature sensitivity, which is a temperature sensitivity hydrogel composite body taking chitosan, chitosan quaternary ammonium and Alpha, Beta-phosphate as carriers, and the carriers are loaded with plasmids containing bone tissue growth factor coding genes. The invention also provides a preparation method forthe regeneration of periodontium tissue of the temperature sensitivity. In the invention, the composite body is planted into a body, target DNA directly transfects target cells and continuously expresses coded growth factors, rebuilds and recovers the growth activity of osteoblast; along with the gradual degradation and absorption of biological support materials, tissues with the original specialstate and function are formed, so that the purpose of alveolar bone regeneration and function rebuilding are achieved. In the invention, the defect that the half-life period is short due to the direct application of an exogenous growth factor is overcome, and the barrier of immunological rejection as the exogenous cells are planted into the body is broken through, therefore, the clinical application value and the clinical application potential are exact.

Description

Technical field [0001] The invention relates to a temperature-sensitive periodontal tissue regeneration inducer that is used as a tissue engineering scaffold and can realize gene targeted therapy and bionic controlled release, and a preparation method of the regeneration inducer. Background technique [0002] Early research on temperature-sensitive hydrogels mainly focused on the synthesis of temperature-sensitive hydrogel materials, including polyethylene glycol / polypropylene glycol copolymer, polyethylene glycol / polybutylene glycol copolymer, polyacrylic acid and polyisocyanurate Preparation of a series of polymers with phase change characteristics such as propyl acrylamide. However, most of these polymers are not biodegradable, which limits their clinical applications. Subsequent to the emergence of biodegradable and injectable alternative products under physiological conditions, such as polyoxyethylene (PEG) and polylactic acid (PLA) interpolymers and terpolymers (PEG-PLA-PE...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/54A61L27/20A61L27/52A61K38/18A61K48/00A61P43/00
CPCY02A50/30
Inventor 吉秋霞宋文斌
Owner HOSPITAL ATTACHED TO QINGDAO UNIV
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