112results about How to "Excellent temperature sensitive performance" patented technology

This invention relates to a method for prparing temperature-sensitive polyvinylidene flourine intelligent membrane. The method is: NIPA as grafted monomer, PVDF as macromelocular initiator, cuprous chloride as catalyst,4,4 demethyl 2,2 bipyridine as ligand, using atom transfer free radical process, preparation of temperature-sensitive polyvinylidene flourine intelligent membrane comprising: in reaction kettle, adding 7-13% PVDF(WT%) and solvent N-methyl-2-pyrrolidone,heating to 50 deg. C to make it solve completely; then adding grafted monomer NIPA, its mol ratio with cuprous chloride is 2000:1-2500:1,filling nitrogen gas 30mins. at stirring, and separately adding 0.20-025g ligand 4,4-dimethyl 2,2-bipyridine and 0.04-0.06g catalyst cupous chloride, stirring and heating to 80-120deg.C,constant reaction 19-25 hrs; then washing reaction product using pure water to obtain light brown solid, filtering and 80 C degree drying to obtain finish product.

The invention discloses a decomposable temperature-sensitive chemical crosslinking gel and making method in the macromolecular material technical domain, which is characterized by the following: adopting PEG as hydrophilic block and decomposable polyester as hydrophobic block to form the host of temperature sensitive amphipathy block copolymer; forming gel under human body temperature (or normal temperature) in the water; using macromolecular monomer technique to obtain stable chemical crosslinking gel as drug slow-release carrier or culture rack of tissue engineering cell.

The invention relates to the technical field of biological materials, and particularly relates to thermo-sensitive chitosan derivative-hydroxypentyl chitosan and a preparation method of the hydroxypentyl chitosan. The invention provides the preparation method of the thermo-sensitive chitosan derivative-hydroxypentyl chitosan. The method comprises the steps of firstly carrying out alkali activation on purified chitosan, then taking 1,2-pentane oxide as an etherifying agent, taking isopropanol as a dispersed phase, carrying out mild reaction for 3-6 days at room temperature, participating with acetone and washing until a neutral state reaches after reaction, carriying out vacuum drying in at 50 DEG C to obtain the hydroxypentyl chitosan. The hydroxypentyl chitosan disclosed by the invention has water-solubility and thermosensitivity; the lowest critical solution temperature (LCST) is 17 DEG C (2%W / V), and the hydroxypentyl chitosan can be fast cured into a stable gel state from a liquid state at physiological temperature of 37 DEG C.

The invention discloses a preparation method of gel nano particles in a thermosensitivie nuclear shell structure and an application of a product thereof. Thermosensitive microgel is prepared by the preparation method by using polybutadiene nanometer emulsion particles as nucleus, grafting poly(N-isopropyl acrylamide) on the surface of the nucleus by a free radical polymerization method under the effect of a cross-linking agent. The microgel can be used as a nano reactor. Nano silver particles with controllable particle size and distribution are prepared through an in-situ reduction reaction. The microgel carrying the nano silver particles can be used as a high-performance catalyst, a bacteriacide and the like.

The invention relates to hydrogel as well as a preparation method and application thereof. The hydrogel is patterned temperature-sensitive hydrogel which comprises the following raw material components in parts by mass: 10-50 parts of an N-isopropylacrylamide monomer, 0.01-0.2 part of graphene oxide and 5-30 parts of a cross-linking agent. The hydrogel is applied to cell culture and collection ofcell layers and can be further used for establishing three-dimensional tumor models. The hydrogel provided by the invention is reasonable in raw material component matching and excellent in temperature sensitivity; due to reasonably matched concentrations of graphene oxide, the hydrogel has hydrophobicity at a cell culture temperature, and adhesion and propagation of cells on the surface of the hydrogel can be promoted; under a room temperature condition, the hydrogel has hydrophilcity instead of hydrophobicity, peeled cell layers can be collected with a short time, the obtained cell layers can be further cultured into cell spheres of three-dimensional structures, and the cell spheres can be applied to tumor model study and used as cell models for screening anti-tumor medicines.

The invention discloses a semi-interpenetrating network nano hybrid hydrogel and a preparation method and an application thereof. Firstly, chitosan is modified to prepare water soluble carboxymethyl chitosan (CMCS); then N-isopropylacrylamide (NIPAM), CMCS and (carboxyl functionalized) carbon nanotubes are subjected to in-situ free radical crosslinking polymerization reaction, and the semi-interpenetrating network nano hybrid hydrogel with different composition ratios of NIPAM, CMCS and the nanotubes is obtained. The prepared novel nano hybrid hydrogel has relatively high swelling rate, appropriate volume phase transition temperature and pH phase transition point, and high drug loading amount and continuous controllable drug release behavior. The hydrogel pharmaceutical preparation can be used as a promising hydrophilic oral drug carrier, is used for targeted release of oral vaccines, polypeptides, proteins and anticancer drugs, has low toxicity, and can be used for long-term treatment of colon cancer and the like.

The invention discloses a preparation method of a biological multilayer nerve conduit containing mesenchymal stem cells. The method comprises the following steps: (1) dissolving a photocurable biological hydrogel with a PBS solution containing a photoinitiator to obtain two concentrations of biological ink; (2) collecting mesenchymal stem cells with confluence degree of more than 80%, and resuspending the mesenchymal stem cells with low concentration of biological ink after treatment to obtain cell-loaded biological ink; 3) load that cell-loaded biological ink and the high-concentration biological ink into two nozzles of the biological 3D printing device respectively; (4) setting printing parameters, firstly printing the cell-carrying biological ink on the optical axis rotating uniformly to obtain a first layer of nerve conduit; And then printing high-concentration biological ink to obtain a double-layer hollow tubular nerve conduit; (5) Biological multilayer nerve conduit was obtainedby ultraviolet irradiation. The invention has the advantages of short preparation time, simplicity, mild operation conditions, simple structure, good biocompatibility and degradability of the prepared nerve conduit.

The preparation method includes two steps of dispersing macromolecular monomer solution to be microdroplets under temperature of phase shift for macromolecular monomer solution and then rising temperature to cause polymerization.

The invention provides a polymer, a preparation method and a poly-N-isopropylacrylamide europium complex micelle, and belongs to the field of europoium complexes. The polymer is methoxypolyethylene glycol-poly-N-isopropylacrylamide-polymethylacrylic acid N,N-diethylaminoethyl, the structural formula is shown as the formula I, and the abbreviate formula is mPEG23-PNIPAM-PDEA, wherein mPEG23 is methoxypolyethylene glycol, PNIPAM is poly-N-isopropylacrylamide, and PDEA is polymethylacrylic acid N,N-diethylaminoethyl. The structural formula of the europium complex micelle is shown as the formula II. Due to the fact that the polymer (mPEG23-PNIPAM-PDEA) has good thermosensitivity and pH sensibility, the polymer can be used as a chemical conveying carrier after being prepared into the europium complex micelle.

The invention relates to a pramipexole sustained-release preparation for injection and a preparation method thereof, belonging to the technical field of medicines. The preparation method comprises thefollowing steps: dissolving a main drug and an internal water phase additive into water for injection so as to form a main drug-containing internal water phase solution; dissolving PLGA and an organic phase additive into an organic solvent so as to form an organic phase solution; mixing the main drug-containing internal water phase solution with the organic phase solution and carrying out ultrasonic treatment so as to form a primary water-in-oil emulsion; adding the primary water-in-oil emulsion into an external water phase solution, and carrying out stirring so as to form a water-in-oil-in-water multiple emulsion; solidifying the water-in-oil-in-water multiple emulsion so as to form microspheres, carrying out centrifugal washing, and collecting the microspheres so as to obtain wet microspheres; adding a gel solution and a freeze-drying protective agent into the wet microspheres so as to obtain a microsphere gel solution; and subjecting the microsphere gel solution to freeze-drying. The pramipexole sustained-release preparation for injection provided by the invention has high drug loading capacity and good temperature sensitivity and biological compatibility, sustainably and stably releases drugs, can effectively reduce the occurrence of motor complications, and improves the compliance of a patient.

The invention provides an inductive agent for regeneration of periodontium tissue of temperature sensitivity, which is a temperature sensitivity hydrogel composite body taking chitosan, chitosan quaternary ammonium and Alpha, Beta-phosphate as carriers, and the carriers are loaded with plasmids containing bone tissue growth factor coding genes. The invention also provides a preparation method forthe regeneration of periodontium tissue of the temperature sensitivity. In the invention, the composite body is planted into a body, target DNA directly transfects target cells and continuously expresses coded growth factors, rebuilds and recovers the growth activity of osteoblast; along with the gradual degradation and absorption of biological support materials, tissues with the original specialstate and function are formed, so that the purpose of alveolar bone regeneration and function rebuilding are achieved. In the invention, the defect that the half-life period is short due to the direct application of an exogenous growth factor is overcome, and the barrier of immunological rejection as the exogenous cells are planted into the body is broken through, therefore, the clinical application value and the clinical application potential are exact.

The application provides a thermosensitive polymer having a structure as shown in formula (I). The thermosensitive polymer takes dipentaerythritol as a core, and is prepared by enabling the dipentaerythritol to react with an acylating agent so as to obtain an initiator, then carrying out atom transfer radical polymerization on the initiator and a thermosensitive monomer to obtain an active thermosensitive polymer with uniform chain length and controllable molecular weight, and finally, carrying out an azido reaction on the tail end of the polymer to obtain the thermosensitive polymer having an azide group at the tail end. Compared with the prior art, the polymer provided by the application has the characteristics of being simple, convenient and easy to implement, has controllable arm number and arm length, and is flexible and adjustable in structure and convenient to modify; furthermore, the thermosensitive polymer has good thermosensitivity and a use scope close to environment temperature, thus having good application prospect.

The invention relates to the technical field of high molecular materials, and particularly discloses a composite temperature-sensitive aquagel and a preparation method thereof. The preparation method of the composite temperature-sensitive aquagel comprises the following steps: copolymerizing 6-desoxy-6-amino-cyclodextrin with chitosan carboxyl derivatives to obtain a water-soluble cyclodextrin-chitosan derivative copolymer, dissolving the water-soluble cyclodextrin-chitosan derivative copolymer in water to prepare a solution A, adding active substances into the solution A, and dissolving by stirring to obtain a solution B; dissolving glycerophosphate sodium in water to prepare a solution C; and cooling the solution B and the solution C, and adding the solution C into the solution B under stirring conditions, thereby obtaining the composite temperature-sensitive aquagel. The composite temperature-sensitive aquagel disclosed by the invention has the advantages of favorable temperature sensitivity, favorable biocompatibility and good slow release performance; and the preparation method has the advantages of stable technique, wide raw material sources and low manufacturing cost, and is simple to operate.

The invention discloses a fluorine-containing block graft polymer with thermosensitivity and a preparation method and application thereof. According to the invention, fluorine-containing acrylate monomers are polymerized into a macroinitiator by adopting atom transfer radical polymerization; the macroinitiator and glycidyl methacrylate are copolymerized; and after an azide group is introduced into a side chain epoxide cleavage of the obtained product, a polymer with thermosensibility is grafted by adopting the click chemical reaction so as to obtain the block graft polymer. The fluorine-containing block graft polymer has mild reaction conditions, is easy to operate and has high reaction speed and high conversion rate. The preparation method of the fluorine-containing block graft polymer with thermosensitivity, which is disclosed by the invention, is simple and convenient to operate, and has low toxicity and high product purity; and the prepared fluorine-containing block graft polymer with thermosensitivity not only has the advantages of fluorine-containing materials, but also has excellent performance of thermosensitivity and realizes function combination.

The invention relates to a PEG type thermosensitive hydrogel three-dimensional cell scaffold and as well as a preparation method and application thereof. The preparation method of the three-dimensional cell scaffold comprises the steps of mixing acrylate water-soluble macromolecular polymerizable monomer containing a PEG side chain, an initiator, a surfactant, a cross-linking agent and / or comonomer, reacting and obtaining PEG type thermosensitive nano gel; mixing the PEG type thermosensitive nano gel with cells uniformly at room temperature, and forming the PEG type thermosensitive hydrogel three-dimensional cell scaffold by thermally inducing in-situ embedded cells. The preparation method is easy to conduct, is featured by mild conditions, and damages cells slightly. Compared with a traditional PNIPAM type thermosensitive hydrogel scaffold, the PEG type thermosensitive hydrogel scaffold has high biocompatibility, is degradable, is adjustable in nature, prolongs syneresis slightly along with time, and can keep cells growing and proliferating for long time, thereby being an ideal tissue engineering material, and being used in fields like in vitro culture of cells, tissue repair andtumor treatment.

The invention discloses an intelligent graphene nano-material with high chiral selectivity as well as preparation and application thereof. The material is prepared from graphene, a temperature-sensitive component, a magnetic component and the like. The material disclosed by the invention has high chiral selectivity, temperature-sensitive performance and magnetic responsiveness; the material has the advantages of simplicity in operation, high resolution efficiency, convenience for recycling materials, and environment-friendly operation process in chiral resolution of an amino acid enantiomer, and has a good industrial application prospect.

The invention provides temperature-sensitive waterproof moisture-permeable shell fabric and a preparation method thereof. The preparation method includes soaking base cloth in hydrochloric acid for hydrolysis, wherein the base cloth is shell fabric which can contain an amino and / or hydroxy structure after being hydrolyzed; soaking the hydrolyzed base cloth in a glutaric dialdehyde solution for pre-crosslinking reaction, and subjecting the pre-crosslinked base cloth to padding treatment in temperature-sensitive hydrogel so as to obtain crosslinked base cloth; applying the surface of the crosslinked base cloth with a coating agent and baking so as to obtain the temperature-sensitive waterproof moisture-permeable shell fabric. The temperature-sensitive waterproof moisture-permeable shell fabric has the advantages of good temperature-sensitive performance and waterproof moisture-permeable performance as well as excellent washability because of good bonding performance and high bonding firmness between the temperature-sensitive hydrogel and nylon fabric.

The invention discloses a preparation method of a cellulose high efficient antibacterial material. The preparation method is characterized by comprising the following steps: (1) preparing modified cellulase; (2) preparing cellulose powder; (3) preparing cellulose; and (4) preparing the antibacterial material. The modified cellulase can maintain a high catalytic activity during the reaction process; the used cellulose has the advantages of renewable property and good biocompatibility; the polymer can provide an effective and nontoxic antibacterial activity in a water-expandable material; a silver nitrate water solution and cellulose are mixed, then the mixture is allowed to stand still for a while and processed by microwaves in the absence of light, the obtained material has an antibacterial activity, at the same time, due to the added silver ions, the material cannot be degraded by light easily and becomes non-photosensitive; and a nano silver antibacterial material loaded on the obtained cellulose can be widely in following fields: nano enhanced materials, antibacterial surgical gowns, wound dressing, controlled release of drugs, textile coating, and the like.

The invention discloses an alkoxy ethers temperature-sensitive polymer nanometer microsphere and a preparation method thereof. The method includes the steps of first catalyzing two monomers for copolymerization through rhodium metal to obtain a temperature-sensitive polymer; dissolving the polymer in an acidic aqueous solution, and adding a corresponding cross-linking agent to obtain the intelligent nanometer microsphere. The nanometer microsphere has good dispersibility in an aqueous solution and shows good temperature-sensitive behavior. The size of the nanometer microsphere can be reversibly regulated through external temperature stimuli. The alkoxy ethers temperature-sensitive polymer nanometer microsphere and the preparation method thereof provide a theoretical basis for the construction of the nanometer microsphere through a dynamic keys crosslinked temperature-sensitive polymer, have an important academic value in the preparation of novel intelligent nanometer materials and are instructive for the expansion of the application of the stimulus responsive temperature-sensitive polymer in the fields of materials science, drug sustained release and envelope.

The invention discloses thermosensitive, conductive and super-tensile collagen-based instant self-healing hydrogel. Guar gum and N-isopropyl acrylamide are led into collagen, and in-situ polymerization is performed to form the hydrogel with a triple structure, so that the hydrogel has a certain strength and thermosensitivity. Oxidized graphene is added, the conductivity of the collagen-based hydrogel can be further improved, the oxidized graphene and a macromolecule network form more reversible physical cross-linking points, and the toughness (tensile property) of the hydrogel is further enhanced. Ion cross-linking action is introduced into collagen and guar gum macromolecules to generate a large quantity of reversible binding sites, and an energy dissipation mechanism is given, so that the hydrogel has good tensile property and self-repairability. A feasible way is provided for preparation of the thermosensitive, conductive and super-tensile collagen-based self-healing hydrogel.

The invention relates to collagen for preparing hydrogel and a preparation method of the collagen. A collagen sequence containing a bioactive structure GFPGER region is designed, cysteine with an oxidative crosslinking characteristic is introduced into a plurality of sites of the collagen sequence, and four kinds of cysteine-containing collagen with higher purity are obtained through heterologousexpression of escherichia coli. Hydrogen peroxide is used as a crosslinking agent for crosslinking the collagen to prepare the hydrogel. The prepared collagen has good thermosensitivity, can form stable hydrogel construction in an oxidation environment, has no toxicity to cells, and provides a good basis for the application of a drug controlled release carrier.

The invention relates to the field of functional macromolecular materials, in particular to novel linear temperature-sensitive type polyurethane and a preparation method thereof. The preparation method of polyurethane comprises steps as follows: polycarbonate diol and dimethylolpropionic acid are added to an organic solvent and are mixed uniformly at the temperature of 75-85 DEG C under the protection of nitrogen, then hexamethylene diisocyanate and a catalyst are added, the mixture is stirred at the temperature of 75-85 DEG C and reacts for more than 3 h to generate linear polyurethane with isocyanato as an end group, ethylenediamine and triethylamine are added, the mixture is stirred to react for 2 h or longer, linear polyurethane with amino as an end group is obtained, N-isopropyl acrylamide is added, the mixture is stirred to react for 12 h or longer, then deionized water is added, an organic solvent is removed through rotary evaporation in a vacuum state, and the novel linear temperature-sensitive type polyurethane is obtained. Two ends of a polyurethane chain segment of the novel linear temperature-sensitive type polyurethane are directly sealed with N-isopropyl acrylamide, no other chain segments are contained, the synthetic method is simple, the synthesis and chemical grafting steps of poly(N-isopropyl acrylamide) are omitted, synthetic raw materials are also saved, and the cost is reduced.

The invention discloses a method for preparing a structure controlled temperature-sensitive polyvinylidene fluoride (PVDF) flat separation membrane and a product thereof, belongs to the technical field of membranes, and particularly relates to an intelligent polymeric membrane technology. According to the structure controlled temperature-sensitive polyvinylidene fluoride flat separation membrane, the flat separation membrane has temperature response property at the temperature of between 27 and 32 DEG C, and the flat separation membrane can be prepared by the following method of preparation of the PVDF flat membrane and temperature-sensitive modification of the PVDF flat membrane. The method prepares the temperature response PVDF flat separation membrane, the pure water flux of the membrane is abruptly increased at the temperature of 27 to 32 DEG C, the interception rate of the membrane on bovine serum albumin and the like is abruptly reduced, and the abrupt change amplitude reaches over 40 percent. The product is simple in process and low in cost, does not need special equipment, is easy for industrialized implementation, and has good membrane temperature sensitivity.

The invention relates to waterborne thermosensitive polyurethane and a preparation method thereof. The preparation method comprises the following steps: S1, adding N-isopropylacrylamide and diethanolamine into a reaction vessel in proportion so as to obtain a dihydroxy containing thermosensitive monomer molecule B2 under proper conditions; and S2, dissolving polycarbonate diol and dimethylolpropionic acid in acetone in a reaction vessel equipped with a reflux condenser in proportion, carrying out heating, carrying out uniform mixing under stirring, then adding toluene diisocynate and a catalyst, carrying out a reaction so as to generate polyurethane, then adding the thermosensitive monomer B2 obtained in the S1, carrying out a reaction for 2 h or above, adding triethylamine and carrying out neutralizing, then adding water and carrying out emulsifying, and carrying out rotary evaporation and removing the acetone so as to obtain the waterborne thermosensitive polyurethane. The inventionhas the following beneficial effects: the dihydroxy containing thermosensitive monomer molecule is firstly employed to combine with a polyurethane chain segment; the thermosensitive monomer B2 is distributed at the end part and central part of finally-obtained thermosensitive polyurethane; the molecular weight of the obtained thermosensitive polyurethane is not restricted; thus, the waterborne thermosensitive polyurethane with long chain length can be obtained.

The invention provides a temperature-sensitive polymer single-ion conductive smart gel and a preparation method thereof. While the temperature-sensitive polymer single-ion conductive smart gel has obvious temperature sensitivity, ionic conductivity tends to decrease near the volume phase transition temperature range of polymer as temperature increases. The preparation method is as follows: metal salt of 2-acrylamide-2-methylpropanesulfonic acid and vinyl temperature-sensitive monomer are copolymerized, so that the temperature-sensitive polymer single-ion conductive smart gel is prepared, the molar ratio of the vinyl temperature-sensitive monomer to the metal salt of 2-acrylamide-2-methylpropanesulfonic acid is 99.9:0.1 to 90:10, and the metal salt of 2-acrylamide-2-methylpropanesulfonic acid is the lithium salt, sodium salt or potassium salt of 2-acrylamide-2-methylpropanesulfonic acid.

The invention relates to a method using thermo-sensitive segmented copolymer to modify a nano-diamond. The method includes the steps of firstly, preparing the thermo-sensitive segmented copolymer; secondly, preparing the nano-diamond carrying an isocyanate group; thirdly, covalently grafting the thermo-sensitive segmented copolymer to the surface of the nano-diamond. The method has the advantages that the isocyanate group (-NCO) with high reaction activity is used as a connecting bridge, one end of the isocyanate group reacts with the carboxyl (-COOH) and hydroxyl (-OH) on the surface of the nano-diamond, the other end of the isocyanate group reacts with the hydroxyl in the segmented copolymer, and accordingly the thermo-sensitive segmented copolymer with both hydrophilicity and hydrophobicity is grafted to the surface of the nano-diamond, and the nano-diamond is allowed to have good dispersity and stability in both organic solvents and aqueous solvents.

The invention discloses composite hydrogel, a preparation method and application thereof, and belongs to the technical field of biology. The preparation method of the composite hydrogel comprises thefollowing steps: dissolving chitin in an alkali and / or urea solution, and carrying out etherification modification on the chitin to obtain hydroxypropyl chitin (HPCH); uniformly mixing the HPCH and hyaluronic acid (HA) to prepare a gel aqueous dispersion, pouring the gel aqueous dispersion in a glass container, placing the glass container in a refrigerator, and performing freeze-drying to obtain gel powder; dissolving the gel powder in a PBS, performing uniform ultrasonic mixing, and then placing the obtained mixture in the refrigerator overnight for swelling; and after swelling, adding powdercontaining Mg<2+>, and performing uniform stirring and then ultrasonic dispersion to obtain Mg<2+> / HA / HPCH composite hydrogel. The composite hydrogel can be used for preparing 3D printing bio-ink, printing in vivo can be realized according to designed shapes and sizes, the sprayed bio-ink can be formed at a specified part, and cartilage defects can be repaired individually.

The invention provides a thermo-sensitive hydrogel and a preparation method thereof. The method comprises the following steps: dissolving a thermo-sensitive monomer and a cross-linking agent in a solvent, and carrying out a polymerization reaction through the irradiation of ultraviolet lights in the presence of a photoinitiator, so as to obtain the thermo-sensitive hydrogel, wherein the photoinitiator has a light bleaching effect, and the cross-linking agent is a substance with a bifunctional degree. According to the method disclosed by the invention, an external heating source is not needed,only the irradiation of the ultraviolet lights is needed, the reaction time is short, the pollution is avoided, the energy is saved, the environment is protected, the preparation method is efficient and controllable, and the prepared hydrogel has good thermal sensitivity, thermal stability and mechanical property. The poly(N-isopropylacrylamide) hydrogel with thermal sensitivity prepared by the embodiment of the invention has a diameter of about 10mm, the length can reach 80mm, the swelling rate at 25 DEG C is 400-1100%, the low critical phase transition temperature is 30-32 DEG C, and obviousthermal sensitivity is achieved.

The invention relates to thermosensitive graphite oxide composite aquagel and a preparation method thereof and belongs to the technical field of chemical engineering in high polymers. The method is specifically implemented according to the following steps: firstly, preparing graphite oxide by an improved Hummers method, then, dispersing the graphite oxide and a polymerization monomer into an aqueous solution under ultrasonic assistance, and carrying out a heat-preserving reaction, thereby obtaining thermosensitive poly(acrylic acid / diacetone acrylamide / acrylamide) / graphite oxide composite aquagel. During the preparation of the aquagel, multi-step reaction is not required, N2 introducing treatment is not required, and the method is easy and feasible. The composite aquagel prepared by the method has the advantages of appropriate water retention property, good thermal shrinkage sensitivity and the like.