The invention discloses a transparentizing
reagent and application thereof in
biological tissue material
optical imaging as well as a
living body skin tissue transparentizing imaging method. The transparentizing
reagent is
mannose or a mixture formed by dissoving
mannose and 1,3-dimethyl-2-imidazolinone in a
solvent. The transparentizing
reagent can be applied to the
biological tissue material
optical imaging. The
living body skin tissue transparentizing imaging method comprises the following steps: removing hairs on the surface, cleaning and
drying; fixing and placing a
skin tissue on a focalplane of an
optical imaging system; dropwise adding the transparentizing reagent to the
skin tissue at an imaging position, and standing for 5-30 minutes; imaging; and after the imaging is finished,wiping the transparentizing reagent, and repeatedly scrubbing and cleaning the surface of the
skin tissue with normal
saline or
phosphate buffer solution, so that the
skin tissue restores the originalstate. The reagent disclosed by the invention can not produce obvious
tissue expansion or tissue shrinkage when in use, a detailed structure of a biological material can be completely stored, transparentizing time is short, transparentizing capability is high, and the reagent can be applied to the
biological tissue material optical imaging.