A refrigerator based on micro-channel regenerative heat exchange technology and Joule-Thomson effect is machined by the aid of atomic diffusion fusion welding technology and comprises an inlet-outlet portion, a regenerative heat exchange portion, a throttling portion and an evaporating cavity. The inlet-outlet portion is provided with an inlet and an outlet, the regenerative heat exchange portion comprises at least one high-temperature layer and at least one low-temperature layer which are pairwise adjacent, each layer is provided with at least one channel, high-temperature and high-pressure gas enters the channels of the high-temperature layers from the inlet, then enters the throttling portion and is cooled in a throttling manner once, and then the high-temperature and high-pressure gas flows to the evaporating cavity with a large space and accordingly is cooled in the throttling manner again. Owing to throttling and cooling, part of the gas is condensed into liquid, the rest gas is low-temperature and low-pressure gas, the liquid and the low-temperature and low-pressure gas are mixed together to form a low-temperature and low-pressure gas liquid mixture, the evaporating cavity keeps the low temperature by the aid of the low-temperature and low-pressure gas liquid mixture, a high-temperature device connected with the evaporating cavity is cooled by the evaporating cavity, and the gas in the evaporating cavity reflows into the channels of the low-temperature layers and is discharged from the outlet after exchanging heat with the high-temperature and high-pressure gas in the channels of the high-temperature layers adjacent to the low-temperature layers.