Serum-free culture medium for adipose-derived stem cells, and preparation method and application of serum-free culture medium
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A technology of serum-free medium and mesenchymal stem cells, applied in the direction of cell culture active agents, biochemical equipment and methods, culture process, etc., to achieve stable performance, simple preparation process, and increase the effect of amplification speed
Active Publication Date: 2021-12-03
BAOXIN ASIA PACIFIC BIOTECH SHENZHEN CO LTD
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[0005] The purpose of this application is to provide a serum-free medium for adipose-derived mesenchymal stem cells and its preparation method, as well as an application of a serum-free medium for adipose-derived mesenchymal stem cells, aiming to solve the problem of existing adipose-derived mesenchymal stem cells to a certain extent. The problem of using serum-containing medium as a medium for stem cell culture
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[0067] The second aspect of the embodiment of the present application provides a method for preparing a serum-free medium of adipose-derived mesenchymal stem cells, comprising the following steps:
[0068] S10. Obtain transferrin, recombinant human insulin, fibroblast growth factor, fibronectin, astragaloside IV, Codonopsis pilosula extract and aloe extract respectively;
[0069] S20. Mix transferrin, recombinant human insulin, fibroblast growth factor, fibronectin, astragaloside IV, Codonopsis pilosula extract and aloe extract with DMEM / F12 medium to obtain adipose-derived mesenchymal stem cells without serum medium.
[0070] The preparation method of the serum-free medium of adipose-derived mesenchymal stem cells provided in the second aspect of the embodiment of the present application is to obtain transferrin, recombinant human insulin, fibroblast growth factor, fibronectin, astragaloside IV, and Codonopsis pilosula extract respectively After being mixed with aloe vera ex...
Embodiment 1
[0125] A kind of adipose-derived mesenchymal stem cell has / serum-free medium, comprises DMEM / F12 medium, the transferrin of final concentration is 8 μ g / mL, the recombinant human insulin of 10 μ g / mL, the fibroblast growth factor of 10 ng / mL, 50μg / mL of fibronectin, the concentrations of astragaloside IV were 6mg / l, 12mg / l, 24mg / l, 48mg / l and 96mg / l.
Embodiment 2
[0127] A kind of adipose-derived mesenchymal stem cell has / serum-free culture medium, comprises DMEM / F12 medium, the transferrin of final concentration is 15 μ g / mL, the recombinant human insulin of 20 μ g / mL, the fibroblast growth factor of 20 ng / mL, 70μg / mL fibronectin, the concentration of Codonopsis pilosula extract is 5mg / l, 10mg / l, 20mg / l, 40mg / l and 60mg / l respectively.
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Abstract
The invention belongs to the technical field of cell culture media, and particularly relates to a serum-free culture medium for adipose-derived stem cells, and a preparation method and application of the serum-free culture medium for the adipose-derived stem cells, wherein the serum-free culture medium for the adipose-derived stem cells comprises a basic culture medium and additives, wherein the basic culture medium comprises a DMEM/F12 culture medium; and the additives comprise transferrin, recombinant human insulin, fibroblast growth factors, fibronectin, astragaloside, a codonopsis pilosula extract and an aloe extract. The serum-free culture medium disclosed by the invention is used for culturing the adipose-derived stem cells; through the synergistic effect of all the components, growth and proliferation of the adipose-derived stem cells can be promoted; cell damage can be reduced; the survival rate of the cells can be increased; and therefore, the in-vitro amplification capacity of the adipose-derived stem cells is improved. Moreover, heterologous pollution is avoided; potential safety hazards causing rejection reaction do not exist; and the culture medium is stable in performance and reliable in quality.
Description
technical field [0001] The application belongs to the technical field of cell culture medium, and in particular relates to a serum-free medium for adipose-derived mesenchymal stem cells and a preparation method thereof, as well as an application of a serum-free medium for adipose-derived mesenchymal stem cells. Background technique [0002] Stem cells are a type of pluripotent cells with self-renewal and multi-directional differentiation capabilities. Mesenchymal stem cells are an important member of the stem cell family, derived from the mesoderm in the early stage of development, and can differentiate into fat, bone, cartilage, muscle, tendon, ligament, nerve, liver, cardiac muscle, Various tissue cells such as endothelium are ideal seed cells for regenerative medicine. Mesenchymal stem cells exist in bone marrow, umbilical cord blood and umbilical cord tissue, placental tissue, adipose tissue and other tissues. When cultured in vitro, it is easy to adhere to the wall an...
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