Novel preservation solution for alveolar lavage fluid microbial nucleic acid

Abstract

The invention discloses a novel preservation solution for an alveolar lavage fluid microbial nucleic acid. The preservation solution comprises a treatment solution I and a treatment solution II, wherein the treatment solution I comprises a guanidine hydrochloride buffer solution, a nuclease inhibitor, a metabolism inhibitor, an alkali metal halide and glycerol, and the treatment solution II comprises an alkali metal hydroxide and a surfactant. The treatment solution I and the treatment solution II which are separately stored are used as the novel preservation solution for the alveolar lavage fluid microbial nucleic acid, so that the preservation solution can be prevented from generating precipitates, at the same time, different interference impurities can be treated respectively in a targeted manner, alveolar lavage fluid samples can be rapidly stabilized, the activity of nuclease is effectively inhibited, the cell lysis is prevented, at the same time, the free nucleic acid in the alveolar lavage fluid can be well protected, the preservation time is long, the accuracy and convenience of nucleic acid detection are improved, and thereby the preservation solution has great applicationvalue in the detection and diagnosis of tumor diseases, viruses and the like.

Description

technical field [0001] The invention relates to the technical field of molecular biology, and more particularly relates to a novel preservation solution of microbial nucleic acid in alveolar lavage fluid. Background technique [0002] The alveolar lavage fluid is directly inserted into the airway with a fiberoptic bronchoscope to reach the site of inflammation or lesion, inject sterile saline through the fiberoptic bronchoscope, and repeatedly suck the liquid out. The components of alveolar lavage fluid include cells, pulmonary surfactant, inflammatory mediators, growth factors, various proteins, enzymes, lipids, nucleic acids, etc. In clinical practice, alveolar lavage fluid is mainly used for pathogenic microorganism inspection, tumor disease detection and diagnosis, etc. Nucleic acid is the general term for deoxyribonucleic acid (DNA) and ribonucleic acid (RNA). With the development of modern molecular biology, the existence of nucleic acid (including RNA and DNA) has b...

AI Technical Summary

Problems solved by technology

If it cannot be sent for inspection in time, it needs to be stored in a refrigerator at 4°C, and the storage time is only about 24 hours. This is also more stringent for the inspection time and storage conditions of alveolar lavage fluid, which brings more difficulties to clinical testing.

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